Advia 2120 hematology system

Manufactured by Siemens

Sourced in Germany, United States, United Kingdom

The ADVIA 2120 Hematology System is a diagnostic instrument designed for automated blood cell analysis. It provides quantitative measurements of various blood parameters, including red blood cells, white blood cells, and platelets.

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Close spelling variants of this product

ADVIA 2120 (167 citations) ADVIA 2120 system (6 citations) ADVIA® 120/2120 Hematology System (2 citations)

59 protocols using advia 2120 hematology system

Hematological Analysis of Infected Samples

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About 2 ml whole blood was collected aseptically in EDTA tubes and used for assessing the hematological parameters, such as the complete blood count, packed cell volume (PCV), hemoglobin concentration (Hb) and for the presence of pathogen specific IgG responses. Blood samples were analyzed within 3 h of collection using a Siemens ADVIA 2120 Hematology system at the Kansas State Veterinary Diagnostic Laboratory (KSVDL) for complete blood counts. This system provides complete data profile of blood samples for the above listed parameters. The hematological values of each infected group were compared with the values of uninfected controls.
Thick blood smear slides were prepared and stained with Wright’s-Giemsa stain also at the KSVDL and shipped at room temperature to the College of Veterinary Medicine, University of Florida for direct smear analysis. The smears were examined manually for Ehrlichia and Anaplasma inclusions (morulae) within the cytoplasm of WBC or platelets, to assess differential leukocyte counts and for the leukocyte morphology. The percentage of granular lymphocytes observed in each blood smear was then converted to absolute numbers/μl of blood using the corresponding TLC values obtained from the CBC analysis.

Nair A.D., Cheng C., Ganta C.K., Sanderson M.W., Alleman A.R., Munderloh U.G, & Ganta R.R. (2016). Comparative Experimental Infection Study in Dogs with Ehrlichia canis, E. chaffeensis, Anaplasma platys and A. phagocytophilum. PLoS ONE, 11(2), e0148239.

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Automated Platelet Count Assay

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Blood samples collected in EDTA tubes were assessed with the ADVIA 2120 Hematology System (Siemens, Deerfield, IL, USA). Platelet counts were expressed as mean ± SEM. with units of 10⁶ cells·mL⁻¹.

Gorbunov N.V, & Kiang J.G. (2017). Ghrelin Therapy Decreases Incidents of Intracranial Hemorrhage in Mice after Whole-Body Ionizing Irradiation Combined with Burn Trauma. International Journal of Molecular Sciences, 18(8), 1693.

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Automated Measurement of Diagnostic Neutrophil Index

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The blood samples for DNI measurement were transferred to the laboratory department in ethylenediaminetetraacetic acid (EDTA) tubes. The DNI was determined within 1 h of blood sampling.
The DNI is included as part of the routine complete blood count (CBS) test at our hospital. The DNI was calculated using an automatic cell analyzer (ADVIA 2120 Hematology System, Siemens Healthcare Diagnostics, Forchheim, Germany) [10 (link)]. This cell analyzer counts white blood cells (WBCs) in independent myeloperoxidase (MPO) and nuclear lobularity channels. The formula for calculating DNI is as follows: DNI (%) = (neutrophil and eosinophil subfractions measured in the MPO channel by a cytochemical MPO reaction)-(polymorphonuclear neutrophil [PMN] subfraction measured in the nuclear lobularity channel by a reflected light beam). Blood cultures were ordered at the discretion of the primary physician based on the presence of the signs and symptoms of SIRS. Each set of blood samples was inoculated into one aerobic and anaerobic bottle and immediately loaded into a BacT/ALERT 3D Microbial Detection System (bioMerieux, Inc., Durham, NC, USA). Candida species identification was done using the automated Vitek 2 Yeast Biochemical Card (bioMerieux, Inc.).

Park S.Y., Lee J.S., Oh J, & Park J.Y. (2020). Delta neutrophil index as a predictive and prognostic factor for Candidemia patients: a matched case-control study. BMC Infectious Diseases, 20, 396.

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Hematological Analysis of RI and CI

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Blood samples were collected in EDTA tubes at day 30 after RI or CI and assessed with the ADVIA 2120 Hematology System (Siemens, Deerfield, IL). Differential analysis was conducted using the peroxidase method and the light scattering techniques recommended by the manufacturer.

Kiang J.G., Zhai M., Liao P.J., Elliott T.B, & Gorbunov N.V. (2014). Ghrelin Therapy Improves Survival after Whole-Body Ionizing Irradiation or Combined with Burn or Wound: Amelioration of Leukocytopenia, Thrombocytopenia, Splenomegaly, and Bone Marrow Injury. Oxidative Medicine and Cellular Longevity, 2014, 215858.

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Inflammatory Response Monitoring in Animal Model

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Weight, temperature, and clinical status, based on a clinical score (Supplementary Table S1), were recorded daily and at 1 and 2 hours after initiation of LPS infusion. Blood samples were collected via the jugular catheter on day 1, 4, and 7 and subsequently 2 and 9 hours after start of LPS infusion (Figure 1). Blood collected at day 7 served as the end of phase 1 and baseline for phase 2. Blood for bloodgas analysis was collected in heparin-coated syringes and analyzed immediately on an automated blood-gas analyzer (GEM premier 3000, Lexington, KY, USA). Blood for hemogram and biochemical parameters were collected in heparin-coated vials. Biochemical analysis was performed on an Advia 1800 Chemistry System (Siemens Healthcare Diagnostics, Tarrytown, NY, USA). Hematological analysis was performed on an Advia 2120 hematology system (Siemens Healthcare Diagnostics).
Blood for measurement of immune and oxidative stress endpoints in plasma were collected in EDTA-containing tubes. Following separation, erythrocytes were isolated and sample aliquots were stored at -80°C until further analysis.

Baek O., Fabiansen C., Friis H., Ritz C., Koch J., Willesen J.L., Heegaard P.M.H., Lykkesfeldt J., Briend A., Golden M.H., Sangild P.T, & Thymann T. (2020). Malnutrition Predisposes to Endotoxin-Induced Edema and Impaired Inflammatory Response in Parenterally Fed Piglets. JPEN. Journal of parenteral and enteral nutrition, 44(4).

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Cytokine profiling of stimulated PBMCs

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The haematology profile was analysed on an Advia 2120 Hematology System (Siemens Healthcare Diagnostics), and the serum analyses for biochemistry were assayed using an Advia 1800 Chemistry System (Siemens Healthcare Diagnostics). For cytokine concentration, the peripheral blood mononuclear cells were obtained from heparinised blood samples by differential centrifugation using histopaque 1•077 (Sigma-Aldrich). Cells were washed, counted and seeded at 5 × 10 6 cells/ml in RPMI 1640 media supplemented with 2 mM L-glutamine, 10 % heatinactivated fetal calf serum, 100 U/ml penicillin and 100 μg/ml streptomycin. The peripheral blood mononuclear cells were then stimulated with either 1 μg/ml LPS (Escherichia coli O55:B5; Sigma-Aldrich) or mock-treated with PBS as controls. The peripheral blood mononuclear cells were then cultured for 24 h at 37°C and 5 % CO 2 , before the supernatant was harvested and stored at -20°C. Cytokine concentrations were then measured by ELISA using commercial antibody pairs according to the manufacturer's instructions (IL-10 and TNFα -ThermoFisher; IL-6, and IL-1β -R and D Systems).

Amdi C., Pedersen M.L.M., Klaaborg J., Myhill L.J., Engelsmann M.N., Williams A.R, & Thymann T. (2021). Pre-weaning adaptation responses in piglets fed milk replacer with gradually increasing amounts of wheat. The British journal of nutrition, 126(3).

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Blood Cell Analysis in Disease

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Blood samples were collected in EDTA tubes after Sham, wound, RI and CI and assessed with the ADVIA 2120 Hematology System (Siemens, Deerfield, IL). Differential analysis was conducted using the peroxidase method and the light scattering techniques recommended by the manufacturer.

Kiang J., Smith J., Anderson M., Umali M., Ho C., Zhai M., Lin B, & Jiang S. (2019). A novel therapy, using Ghrelin with pegylated G-CSF, inhibits brain hemorrhage from ionizing radiation or combined radiation injury. Pharmacy & pharmacology international journal, 7(3), 133-145.

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Blood Cell Analysis in Disease

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Comprehensive Blood Analysis of Donor Samples

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To investigate possible non-pharmaceutical related differences between the donor groups, a series of blood analyses were performed. Red and white blood cells, platelets, hemoglobin, and hematocrit were quantified for each donor sample with an ADVIA 2120 Hematology System (Siemens Corporation; Malvern, PA) using an aliquot of whole blood freshly collected in EDTA. The remaining blood analyses were performed using plasma prepared by centrifugation of citrated whole blood. The plasma was stored at −80°C. Fibrinogen concentration was measured using a STA Compact Hemostasis System (Diagnostica Stago; Parsippany, NJ), Factor VIII activity was measured using a Behring Coagulation System (Siemens Corporation; Malvern, PA), and Factor XIII levels were measured by ELISA (AssayPro; St. Charles, MO).

Dwyer J.F., McCoy J.A., Yang Z., Husser M., Redl H., Murphy M.A., Wolfsegger M., DiOrio J.P., Goppelt A, & Donovan S. (2014). Thrombin based gelatin matrix and fibrin sealant mediated clot formation in the presence of clopidogrel. Thrombosis Journal, 12, 10.

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Immunological Profiling of Blood Samples

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Hematology and immune cell counts on all blood samples from Exp 2 and 3 were performed on an Advia 2120 hematology system (Siemens Healthcare Diagnostics, USA). In Exp 2 arterial blood gas analysis was performed using a GEM Premier 3000 (Instrumentation Laboratory, USA).
In Exp 3, T cell characterization was performed as previously described (31 (link)). Briefly, blood leucocytes were stained with fluorescent antibodies against porcine CD3, CD4, CD8, and FOXP3. Leucocytes were then analyzed using a BD Accuri C6 flow cytometer (BD Biosciences, USA). T cell subsets were defined as follows: T cells (CD3⁺ lymphocytes), CD4 positive T cells (CD3⁺CD4⁺CD8⁻ lymphocytes), CD8 positive T cells (CD3⁺CD4⁻CD8⁺ lymphocytes) and regulatory T cells (CD3⁺CD4⁺FOXP3⁺ lymphocytes).

Bæk O., Ren S., Brunse A., Sangild P.T, & Nguyen D.N. (2020). Impaired Neonatal Immunity and Infection Resistance Following Fetal Growth Restriction in Preterm Pigs. Frontiers in Immunology, 11, 1808.

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