Mouse α pd l1

Manufactured by Thermo Fisher Scientific

Sourced in United States

Mouse α-PD-L1 is a monoclonal antibody that binds to the PD-L1 (programmed death-ligand 1) protein expressed on the surface of certain cells. This antibody is commonly used in research applications to investigate the role of the PD-1/PD-L1 pathway in various biological processes.

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Lab products found in correlation

Mouse α gm130, by Abcam (1 mentions) Rabbit α cmtm6, by Merck Group (1 mentions) Mopc 173, by BioLegend (1 mentions) Mpc 11, by BioLegend (1 mentions) Mopc 21, by BioLegend (1 mentions) Ht7700 tem, by Hitachi (1 mentions)

Close spelling variants of this product

PD-L1 (55 citations) α-PD-L1 (2 citations)

2 protocols using mouse α pd l1

Comprehensive Immunological Profiling of PD-L1 Expression

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Immunofluorescence: mouse α-EEA1 (1G11, eBioscience), mouse α-PD-L1 (MIH1, eBioscience), rabbit α-PD-L1 (EPFR19759, Abcam), mouse α-LAMP1 (H4A4, eBioscience), mouse α-Calnexin (AF18, Abcam), mouse α-GM130 (169276, Abcam), mouse α-TGN46 (2F7.1, Abcam), mouse α-Rab11 (47/Rab11, BD Transduction Laboratories), mouse α-TfR (236-15375, Thermo-Fisher), rabbit α-CMTM6 (HPA026980, Sigma-Aldrich).
Flow Cytometry: FITC mouse IgG2a κ isotype control (MOPC-173, Biolegend), APC mouse IgG2b κ isotype control (MPC-11, Biolegend), APC mouse IgG1 κ isotype control (MOPC-21), APC mouse α-PD-L1 (29E.2A3, Biolegend), APC mouse α-PD-L1 (MIH1, eBioscience), Alexa Fluor 488 rabbit α-PD-L1 (Abcam), APC mouse α-HLA-ABC (W6/32), Alexa Fluor 488 mouse α-HLA-ABC (W6/32, Biolegend), FITC mouse α-CD8 (HIT8a, BD Pharmingen), PE-Cy7 mouse α-TNFα (MAb11, eBioscience), APC mouse α-PD-1 (J105, eBioscience), PerCp-Cy5.5 mouse α-Perforin (B-D48, Biolegend).
Immunoprecipitation/Immunoblotting: goat anti-PDL1 (AF156, R&D Systems), rabbit α-PD-L1 (E1L3N, Cell Signalling Technology), mouse α-PD-L1 (405.9A11, Cell Signalling Technology), mouse α-PD-L1 (MIH1, eBioScience), rabbit α-CMTM6 (Sigma-Aldrich, HPA026980).

Burr M.L., Sparbier C.E., Chan Y.C., Williamson J.C., Woods K., Beavis P.A., Lam E.Y., Henderson M.A., Bell C.C., Stolzenburg S., Gilan O., Bloor S., Noori T., Morgens D.W., Bassik M.C., Neeson P.J., Behren A., Darcy P.K., Dawson S.J., Voskoboinik I., Trapani J.A., Cebon J., Lehner P.J, & Dawson M.A. (2017). CMTM6 maintains the expression of PD-L1 and regulates anti-tumour immunity. Nature, 549(7670), 101-105.

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Immunogold Labeling of Exosomal PD-L1

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In order to obtain the TEM images for identification of exosomal surface proteins, gold nanoparticles (5–10 nm in diameter) have been extensively used.^[⁸, ¹⁶^] In this study, exosomal PD‐L1 was observed using TEM with immunogold labeling, as reported previously.^[^8a^] Briefly, the purified EXOs were deposited onto pure carbon‐coated grids. For immunogold labeling, EXOs were treated with mouse αPD‐L1 (eBioscience, 14‐5983‐82, San Diego, CA, USA), followed by incubation with antimouse IgG‐conjugated 5 nm gold particles (Sigma‐Aldrich, G7527). After staining with 2% uranyl acetate, the grids were dried at 25 °C and visualized at 100 kV using a Hitachi HT‐7700 TEM (Tokyo, Japan).

Shin J.M., Lee C., Son S., Kim C.H., Lee J.A., Ko H., Shin S., Song S.H., Park S., Bae J., Park J., Choe E., Baek M, & Park J.H. (2021). Sulfisoxazole Elicits Robust Antitumour Immune Response Along with Immune Checkpoint Therapy by Inhibiting Exosomal PD‐L1. Advanced Science, 9(5), 2103245.

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