Ticlopidine hydrochloride

Manufactured by Merck Group

Sourced in United States

Ticlopidine hydrochloride is a chemical compound used as a platelet aggregation inhibitor. It is a prescription medication primarily used to reduce the risk of thrombotic stroke and other vascular events in patients with a history of stroke or transient ischemic attack.

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Lab products found in correlation

Ketoconazole, by Merck Group (5 mentions) Quinidine, by Merck Group (4 mentions) 6β hydroxytestosterone, by Merck Group (3 mentions) S mephenytoin, by Biosynth (3 mentions) 4 hydroxymephenytoin, by Biosynth (3 mentions) Montelukast sodium, by Merck Group (3 mentions) Sulfaphenazole, by Merck Group (3 mentions) α naphthoflavone, by Merck Group (3 mentions) N 3 benzylnirvanol, by Merck Group (3 mentions) Testosterone, by Merck Group (2 mentions) Nicotinamide adenine dinucleotide phosphate sodium salt nadp, by Reanal (2 mentions) Glucose 6 phosphate barium salt g6p, by Reanal (2 mentions) Diclofenac, by Biosynth (2 mentions) 4 hydroxydiclofenac, by Biosynth (2 mentions) 4 methylpyrazole hydrochloride, by Merck Group (2 mentions) Tranylcypromine sulfate, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Streptomycin, by Merck Group (1 mentions) Penicillin, by Merck Group (1 mentions) Quercetin, by Merck Group (1 mentions)

Close spelling variants of this product

Ticlopidine (21 citations) Hydrochlorides (2 citations)

6 protocols using ticlopidine hydrochloride

Cytochrome P450 Enzyme Inhibition Assay

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CypExpress^TM 2C19, 2D6 and 3A4 human kits, quercetin (Q), ticlopidine hydrochloride, quinidine, testosterone, 6β-hydroxytestosterone, ketoconazole, fetal bovine serum (FBS), glutamine, penicillin, streptomycin, pyranine (trisodium 8-hydroxypyrene-1,3,6-trisulfonate), bromosulfophthalein, sulforhodamine 101, sodium orthovanadate, probenecid, 5(6)-carboxy-2′,7′-dichlorofluorescein (CDCF), lucifer yellow (LY), and benzbromarone were purchased from Sigma-Aldrich (St. Louis, MO, USA). Nicotinamide adenine dinucleotide phosphate sodium salt (NADP⁺), and glucose-6-phosphate barium salt (G6P) were from Reanal (Budapest, Hungary). S-mephenytoin, 4-hydroxymephenytoin, dextromethorphan, and dextrorphan were obtained from Carbosynth (Berkshire, UK). Isorhamnetin (IR) and Ko143 were purchased from Extrasynthese (Genay Cedex, France) and Tocris Bioscience (Bristol, UK), respectively. Quercetin-3′-sulfate (Q3′S), quercetin-3-glucuronide (Q3G), and isorhamnetin-3-glucuronide (I3G) were synthetized as described previously [46 (link)].

Mohos V., Fliszár-Nyúl E., Ungvári O., Kuffa K., Needs P.W., Kroon P.A., Telbisz Á., Özvegy-Laczka C, & Poór M. (2020). Inhibitory Effects of Quercetin and Its Main Methyl, Sulfate, and Glucuronic Acid Conjugates on Cytochrome P450 Enzymes, and on OATP, BCRP and MRP2 Transporters. Nutrients, 12(8), 2306.

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In Vitro Metabolic Profiling of 7,8-Dihydroxyflavone

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7,8-dihydroxyflavone (DHF) was obtained from Extrasynthese (Genay Cedex, France). S-mephenytoin, 4-hydroxymephenytoin, diclofenac, 4′-hydroxydiclofenac, and 6-thiouric acid were purchased from Carbosynth (Berkshire, UK). High-performance liquid chromatography (HPLC) grade acetonitrile and methanol were acquired from VWR (Budapest, Hungary). Orthophosphoric acid (85 v/v%), human serum albumin (HSA), racemic warfarin (WAR), naproxen (NAP), testosterone, 6β-hydroxytestosterone, naringenin, ticlopidine hydrochloride, 6-mercaptopurin, allopurinol, CypExpress 2C9 kit, CypExpress 2C19 kit, CypExpress 3A4 kit, and xanthine oxidase (XO; from bovine milk) were obtained from Sigma-Aldrich (Budapest, Hungary). Nicotinamide adenine dinucleotide phosphate sodium salt (NADP⁺) and glucose-6-phosphate barium salt (G6P) were purchased from Reanal (Budapest, Hungary). Glacial acetic acid (99.5 v/v%) was obtained from Fluka (Bucharest, Romania). Stock solutions of DHF, site markers, substrates, and products (2000 μM each) were prepared in dimethyl sulfoxide (spectroscopic grade; Fluka) and stored at −20°C.

Fliszár-Nyúl E., Mohos V., Bencsik T., Lemli B., Kunsági-Máté S, & Poór M. (2019). Interactions of 7,8-Dihydroxyflavone with Serum Albumin as well as with CYP2C9, CYP2C19, CYP3A4, and Xanthine Oxidase Biotransformation Enzymes. Biomolecules, 9(11), 655.

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Cytochrome P450 Enzyme Assay Protocol

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Testosterone, 6β-hydroxyTestosterone, ketoconazole, racemic warfarin, ticlopidine hydrochloride, CypExpress^TM 2C9 kit, CypExpress^TM 2C19 kit, and CypExpress^TM 3A4 kit were purchased from Sigma-Aldrich (St. Louis, MO, USA). Diclofenac, 4′-hydroxyDiclofenac, S-mephenytoin, and 4-hydroxymephenytoin were obtained from Carbosynth (Compton, Berkshire, UK).

Zsidó B.Z., Balog M., Erős N., Poór M., Mohos V., Fliszár-Nyúl E., Hetényi C., Nagane M., Hideg K., Kálai T, & Bognár B. (2020). Synthesis of Spin-Labelled Bergamottin: A Potent CYP3A4 Inhibitor with Antiproliferative Activity. International Journal of Molecular Sciences, 21(2), 508.

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Terbinafine Metabolite Profiling

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All chemical solvents were purchased from Thermo Fisher Scientific (Waltham, MA). The substrate terbinafine hydrochloride and metabolites N-desmethyl-terbinafine hydrochloride, N-methyl-1-naphthyl methylamine hydrochloride, 1-naphthyl methylamine, naphthoic acid, and 1-napthaldehyde were purchased from Millipore-Sigma (Burlington, MA), while N-desmethyl-terbinafine hydrochloride and E-6,6-dimethylhept-2-ene-4-ynal (TBF-A) were obtained from Toronto Research Chemicals (Toronto, ON, Canada). Labeling agents dansyl chloride and dansyl hydrazine and internal standards fluoxetine hydrochloride and dimethyl benzaldehyde were purchased from Millipore-Sigma. Isozyme inhibitors α-naphthoflavone, (+)-N-3-benzylnirvanol, ketoconazole, 4-methylpyrazole hydrochloride, montelukast sodium, quinidine, sulfaphenazole, ticlopidine hydrochloride, and tranylcypromine sulfate were also purchased from Millipore-Sigma. Lastly, recombinant Supersomes CYP2C19 and 3A4 were purchased from Corning (Woburn, MA).

Davis M.A., Barnette D.A., Flynn N.R., Pidugu A.S., Swamidass S.J., Boysen G, & Miller G.P. (2019). CYP2C19 and 3A4 Dominate Metabolic Clearance and Bioactivation of Terbinafine Based on Computational and Experimental Approaches. Chemical research in toxicology, 32(6), 1151-1164.

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In vitro Metabolism of Novel Cannabinoids

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All chemical solvents, salts, and buffers were purchased from Thermo Scientific (Waltham, MA, USA). CYP isozyme inhibitors α-naphthoflavone, (+)-N-3-benzylnirvanol, ketoconazole, montelukast sodium, quinidine, sulfaphenazole, ticlopidine hydrochloride, and tranylcypromine sulfate were purchased from Millipore-Sigma. The substrate (5OH-APINACA) and CYP isozyme inhibitors (1-aminobenzotriazole and 4-methylpyrazole hydrochloride) were purchased from Cayman Chemical (Ann Arbor, MI, USA). NADPH-regenerating system components NADP disodium salt, glucose-6-phosphate dehydrogenase, and glucose-6-phosphate were purchased from Millipore-Sigma (St. Louis, MO, USA), while magnesium chloride salt was purchased from Thermo Fisher Scientific. The substrate 5F-APINACA was provided by Dr. William Fantegrossi. Human liver microsomes pooled from 150 individuals, lot#38296, and P450 contribution of 0.380 nmol/mg, were purchased from Corning (Corning, NY, USA). CD-1 male mouse liver microsomes pooled from 6424 mice, lot#2010017, and P450 contribution of 0.671 nmol/mg, were purchased from Sekisui Xenotech (Kansas City, KS, USA). Internal standard acenocoumarol was purchased from Toronto Research Chemicals (Toronto, ON, Canada). ACD/ChemSketch 2017.2.1 software (Toronto, ON, Canada) was used for rendering structures of molecules.

Crosby S.V., Ahmed I.Y., Osborn L.R., Wang Z., Schleiff M.A., Fantegrossi W.E., Nagar S., Prather P.L., Boysen G, & Miller G.P. (2022). Similar 5F-APINACA Metabolism between CD-1 Mouse and Human Liver Microsomes Involves Different P450 Cytochromes. Metabolites, 12(8), 773.

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Quantitative Analysis of Meloxicam Metabolism

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Chemical solvents (methanol, acetonitrile, and DMSO) and MgCl₂ were obtained from Thermo Fisher Scientific (Waltham, MA). Millipore-Sigma (Burlington, MA) was the source for the following: meloxicam (substrate), glyoxal and methylglyoxal (metabolite standards), 7-hydroxycoumarin (internal standard), cytochrome P450 isozyme inhibitors (1-aminobenzotriazole, α-naphthoflavone, (+)-N-3-benzylnirvanol, ketoconazole, 4-methylpyrazole hydrochloride, montelukast sodium, quinidine, sulfaphenazole, ticlopidine hydrochloride, and tranylcypromine sulfate), and NADPH regenerating system (glucose-6 phosphate dehydrogenase, glucose 6-phosphate, and NADP disodium salt). Sudoxicam (substrate), 5’-hydroxymethyl 5’-desmethyl meloxicam (metabolite standard), fluoxetine hydrochloride (internal standard), and 1,2-diamino-4,5-methylenedioxybenzene dihydrochloride (labeling agent) were obtained from Toronto Research Chemicals (Toronto, ON, Canada). Human liver microsomes pooled from 150 donors (HLM150) and recombinant enzyme Supersomes CYP1A2, 2A6, 2B6, 2C8, 2C9, 2C19, 2D6, 2E1, 3A4 and control prepared from baculovirus-transfected insect cells were purchased from Corning Gentest (Corning, NY).

Barnette D.A., Schleiff M.A., Datta A., Flynn N., Swamidass S.J, & Miller G.P. (2020). Meloxicam methyl group determines enzyme specificity for thiazole bioactivation compared to sudoxicam. Toxicology letters, 338, 10-20.

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