The method recommended by Hemalatha et al. [51 ] was adopted to obtain the extracts, with some modifications. First, 2 g of the freeze-dried powder of different macroalgae was soaked in 40 mL of methanol for 24 h at ambient temperature. Twice more, extraction was performed, with the extracts filtered using Whatmann No. 1 filter paper (Sigma-Aldrich, St. Louis, MO, USA) each time, and the supernatants were kept at 4 °C to perform the analysis of total phenolics and oxidoreductive activity.
Whatmann no 1 filter paper
Manufactured by Merck Group
Sourced in United States
Whatmann No. 1 filter paper is a general-purpose filter paper used for a variety of filtration applications in laboratories. It is a cellulose-based paper with a medium-fast flow rate and is suitable for most common filtration tasks.
Automatically generated - may contain errors
Lab products found in correlation
Crystal violet stain, by Merck Group (1 mentions)
Trypan blue dye, by Merck Group (1 mentions)
Crystal violet, by Merck Group (1 mentions)
Hepes buffer solution, by Lonza (1 mentions)
Trypsin edta, by Lonza (1 mentions)
Dulbecco modified eagle medium (dmem), by Lonza (1 mentions)
Rpmi 1640, by Lonza (1 mentions)
Gentamycin, by Lonza (1 mentions)
L glutamine, by Lonza (1 mentions)
Fetal bovine serum (fbs), by Lonza (1 mentions)
2 protocols using whatmann no 1 filter paper
1
Macroalgae Methanol Extraction
2
Crystal Violet Staining Protocol
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Dimethyl sulfoxide (DMSO), crystal violet, and trypan blue dye were purchased from Sigma (St. Louis, MO, USA). Fetal Bovine serum, DMEM, RPMI-1640, HEPES buffer solution, L-glutamine, gentamycin, and 0.25% Trypsin-EDTA were purchased from Lonza (Basel, Switzerland). crystal violet stain (1%): It was composed of 0.5% (w/v) crystal violet and 50% methanol then made up to volume with ddH2O and filtered through a Whatmann No.1 filter paper purchased from Sigma-Aldrich, Saint Louis, MO, USA.
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