Ab216777

Manufactured by Abcam

Ab216777 is a primary antibody specific for an undisclosed target. It is a rabbit monoclonal antibody provided in liquid form. No further details about its intended use or application are available.

Automatically generated - may contain errors

Lab products found in correlation

Ab129002, by Abcam (1 mentions) Ab216772, by Abcam (1 mentions) Free protease inhibitor cocktail tablet, by Roche (1 mentions) Gtx125926, by GeneTex (1 mentions) Odyssey imaging system, by LI COR (1 mentions) Irdye 800cw, by Abcam (1 mentions) C43 ab128193, by Abcam (1 mentions) Irdye 680rd, by Abcam (1 mentions) F1804, by Merck Group (1 mentions) Ab216776, by Abcam (1 mentions) Edta free protease inhibitor tablet, by Roche (1 mentions) Amersham typhoon, by GE Healthcare (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) Bca protein assay kit, by Beyotime (1 mentions) Gapdh, by Proteintech (1 mentions) Gradient gel, by Bio-Rad (1 mentions) Ab1791, by Abcam (1 mentions) Streptavidin alexa fluor790, by Thermo Fisher Scientific (1 mentions) Odyssey infrared scanner, by LI COR (1 mentions) 0.45 μm nitrocellulose membrane, by Pall Corporation (1 mentions)

3 protocols using ab216777

Visualization of Protein Expression in Mini-Genome Assays

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To visualise protein expression during mini-genome assays, around 500,000 transfected cells were lysed in RIPA buffer (150mM NaCl, 1% NP-40, 0.5% Sodium deoxycholate, 0.1% SDS, 50mM TRIS, pH 7.4) supplemented with an EDTA-free protease inhibitor cocktail tablet (Roche).
Proteins were detected with mouse α-FLAG (F1804, Sigma), rabbit α-Vinculin (AB129002, Abcam), rabbit α-PB2 (GTX125926, GeneTex) and mouse α-NP ([C43] ab128193, Abcam). The following near infrared (NIR) fluorescently tagged secondary antibodies were used: IRDye® 680RD Goat Anti-Rabbit (IgG) secondary antibody (Ab216777, Abcam) and IRDye® 800CW Goat Anti-Mouse (IgG) secondary antibody (Ab216772, Abcam). Western Blots were visualised using an Odyssey Imaging System (LI-COR Biosciences).

Golshani P., Liu X.B, & Jones E.G. (2001). Differences in quantal amplitude reflect GluR4- subunit number at corticothalamic synapses on two populations of thalamic neurons. Proceedings of the National Academy of Sciences of the United States of America, 98(7), 4172-4177.

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Western Blotting Analysis of Testicular Caspase-3

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Testes were
lysed in RIPA lysis buffer (Beyotime, P0013C) containing cOmplete,
EDTA-free protease inhibitor tablets (Roche, 04693 132001) and PMSF
(Beyotime, ST506) and homogenized. The lysates were lysed for 30 min,
and the supernatants were collected. The protein concentrations were
determined using the BCA Protein Assay Kit (Beyotime, P0010S). Western
blotting was performed as a standard protocol. Primary antibodies
used were caspase-3 (#19677-1-AP, Proteintech, America) and GAPDH
(#600041-Ig, Proteintech, America). Secondary antibodies (ab216777,
Abcam; ab216776, Abcam, America) were used separately after washing
with TBST. Signals were captured by an Amersham Typhoon (GE Healthcare
Bio-Sciences AB, Uppsala, Sweden).

Gong S., Zhu S., Zhou P., Cheng C.Y., Li W., Yao W, & Sun F. (2023). Discovering a Reversible Male Contraceptive Agent Derived from Lonidamine. ACS Omega, 8(20), 18245-18254.

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Quantitative Western Blot Analysis

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Protein samples were separated by SDS–PAGE using a 4–20% gradient gel (Bio‐Rad) and then transferred onto a 0.45 μm nitrocellulose membrane (Pall Corporation) using a Trans‐Blot Turbo transfer system (Bio‐Rad). Membranes were blocked in 2% wt/vol Bovine Serum Albumin (BSA) in phosphate‐buffered saline (PBS) solution for 30 min at room temperature (RT), incubated for 1 h at RT with rabbit anti‐Histone H3 (ab1791, 1:5,000; Abcam) diluted in a 2% wt/vol BSA/PBS solution containing 0.01% NaN3. After washing, membranes were then probed with secondary goat anti‐rabbit‐IRDye680RD antibody (ab216777; Abcam) and streptavidin‐Alexa Fluor790 (S11378; Invitrogen), both diluted 1:10,000 in 2% wt/vol BSA/PBS solution for 1 h at RT. Blots were washed and imaged on the LI‐COR Odyssey Infrared Scanner. Images were quantified using GelAnalyzer 19.1 (Lazar & Lazar, n.d. ).

Cohen N., Aviram N, & Schuldiner M. (2023). A systematic proximity ligation approach to studying protein‐substrate specificity identifies the substrate spectrum of the Ssh1 translocon. The EMBO Journal, 42(11), e113385.

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