Pab sez6l2

Manufactured by R&D Systems

Sourced in United States

PAb SEZ6L2 is a polyclonal antibody that specifically recognizes the SEZ6L2 protein. SEZ6L2 is a transmembrane protein that belongs to the seizure protein 6-like family. The antibody can be used to detect and study the expression of SEZ6L2 in various biological samples.

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Lab products found in correlation

Pab sez6l, by R&D Systems (2 mentions) Hrp coupled anti mouse and anti rabbit secondary, by Agilent Technologies (2 mentions) Hbss and b27, by Thermo Fisher Scientific (2 mentions) β actin, by Merck Group (2 mentions) Flag m2, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Psd95, by Cell Signaling Technology (1 mentions) Glur6 7, by Merck Group (1 mentions) Ncam 1, by R&D Systems (1 mentions) Nmdar2b, by Cell Signaling Technology (1 mentions) β tubulin, by Merck Group (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Mms 101p, by Fortrea (1 mentions) Anti v5, by Thermo Fisher Scientific (1 mentions) Ha 3f10, by Roche (1 mentions) Biotinylated goat anti rat igg, by Vector Laboratories (1 mentions) Anti dykddddk, by BioLegend (1 mentions) On targetplus non targeting pool, by Horizon Discovery (1 mentions)

2 protocols using pab sez6l2

Detailed Antibody and Reagent Usage

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The following antibodies were used as follows: monoclonal SEZ6 (Pigoni et al, 2016), polyclonal SEZ6 (Gunnersen et al, 2007), pAb SEZ6L2 (R&D Systems, AF4916), pAb SEZ6L (R&D Systems, AF4804), GluR6/7 (04‐921, Millipore, GluK2 and GluK3 antibodies commercially available are not able to discriminate between these two subunits due to their high homology), NMDAR2b (D15B3, Cell Signaling), anti‐GluR2 (MAB397, Millipore), 3D5 (kindly provided by Robert Vassar), calnexin (Enzo, Stressgen, Farmingdale, NY, USA, ADI‐SPA‐860), β‐tubulin (T8578, Sigma), β‐actin (Sigma, A5316), PSD 95 (2507, Cell Signaling), LDLR (R&D Systems, AF2255), NCAM‐1 (R&D Systems, AF6070), rat mAb Flag M2 (F1804, Sigma), 5F8 anti‐Red (Chromotek), anti‐HA.11 (MMS‐101P, Covance), HRP‐coupled anti‐mouse and anti‐rabbit secondary (DAKO), and HRP‐coupled anti‐goat, anti‐rat, and anti‐sheep (Santa Cruz).
The following reagents and media were used as follows: neurobasal medium, HBSS and B27 (Invitrogen), C3 (β‐secretase inhibitor IV; Calbiochem, 565788, final concentration 2 μM), DMEM (Gibco), and FBS (Thermo Fisher Scientific).

Pigoni M., Hsia H., Hartmann J., Rudan Njavro J., Shmueli M.D., Müller S.A., Güner G., Tüshaus J., Kuhn P., Kumar R., Gao P., Tran M.L., Ramazanov B., Blank B., Hipgrave Ederveen A.L., Von Blume J., Mulle C., Gunnersen J.M., Wuhrer M., Rammes G., Busche M.A., Koeglsperger T, & Lichtenthaler S.F. (2020). Seizure protein 6 controls glycosylation and trafficking of kainate receptor subunits GluK2 and GluK3. The EMBO Journal, 39(15), e103457.

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Characterization of SEZ6 Family Proteins

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The following antibodies were used: pAb SEZ6 [18 (link)], newly generated monoclonal SEZ6 and monoclonal SEZ6L (described below), pAb SEZ6L2 (R&D Systems, AF4916), pAb SEZ6L (R&D Systems, AF4804), 3D5 (kindly provided by Robert Vassar), pAb BACE2 (Santa Cruz, sc-10049), calnexin (Enzo, Stressgen, Farmingdale, NY, USA, ADI-SPA-860), β actin (Sigma, A5316), LDLR (R&D system, AF2255), rat mAb HA 3F10 (Roche, 11867423001), Flag M2 (Sigma, F1804), anti-DYKDDDDK (Biolegend, L5), anti-V5 (ThermoFisher, R960-25), HRP coupled anti-mouse and anti-rabbit secondary (DAKO), HRP coupled anti-goat, anti-rat and anti-sheep (Santa Cruz), biotinylated goat anti-rat IgG (Vector Laboratories), SULFO-TAG labelled anti-sheep (MSD, R32AI-1). The following reagents and media were used: neurobasal medium, HBSS and B27 (Invitrogen), C3 (β-secretase inhibitor IV; Calbiochem, 565788, final concentration 2 μM), DAPT (D5942 Sigma, final concentration 1 μM), ON-TARGETplus Bace2 siRNA SMARTpool, ON-TARGETplus Non-targeting Pool (Dharmacon, L-040326-00-0005 and D-001810-10-05, respectively), FlexiTube GeneSolution siRNA for Bace1 and AllStars Negative Control siRNA (Qiagen, GS23821 and SI03650318, respectively).

Pigoni M., Wanngren J., Kuhn P.H., Munro K.M., Gunnersen J.M., Takeshima H., Feederle R., Voytyuk I., De Strooper B., Levasseur M.D., Hrupka B.J., Müller S.A, & Lichtenthaler S.F. (2016). Seizure protein 6 and its homolog seizure 6-like protein are physiological substrates of BACE1 in neurons. Molecular Neurodegeneration, 11, 67.

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