NiNLPs (35% Ni-Lipid and 65% DOPC) were labeled with AF750 as described above. Groups of three mice were then immunized with 50 µg of NLP via i.p., i.n., intravenous (i.v.) intramuscular (i.m.), and subcutaneous (s.c.) administration. After predetermined time points, the mice were euthanized, and spleen, kidney, liver and lungs were removed. The fluorescence intensities in the excised organs were analyzed using a Kodak image station 4000R digital imaging system (Rochester, NY) at excitation and emission wavelengths of 750+/−20 nm and 790+/−20 nm, respectively. The fluorescence values of each organ were then normalized to the organ weight after subtracting for background fluorescence. The background fluorescence of each organ was determined from organs that had been harvested from control mice inoculated with PBS. All experiments involving animals were conducted after review and approval by the Institutional Animal Care and Use Committee at Lawrence Livermore National Laboratory.
Image station 4000r digital imaging system
Manufactured by Kodak
Sourced in United States
The Image Station 4000R is a digital imaging system designed for professional use. It captures high-quality digital images and provides essential functionality for image processing and management. The system features a range of advanced capabilities to facilitate efficient digital imaging workflows.
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Lab products found in correlation
2 protocols using image station 4000r digital imaging system
1
Biodistribution of Fluorescent Lipid Nanoparticles
2
In Vivo Imaging of Joint Injury
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MMPSense (PerkinElmer, Waltham, MA, USA; NEV10168) was administered intravenously 5 hours after injury. Animals (PBS control [n = 5], IA experimental [n = 8]) were euthanized, skin was removed, and the joints were scanned using a Kodak (Rochester, NY, USA) image station 4000R digital imaging system at excitation and emission wavelengths of 750 ± 20 nm and 790 ± 20 nm, respectively. The fluorescent intensity of the uninjured knee joint was used as background and the fluorescent intensity of the injured knee reported here was background subtracted from the uninjured knee joint. Ten‐week‐old WT male mice were injured, received three doses of recombinant mouse Sost (rmSost, R&D Systems 1589‐ST‐025/CF; 4 μg/kg) intra‐articularly (10 μL volume) starting 4 hours after injury, and in vivo imaging was taken in the same manner as MMPSense. Refer Fig. 4 A for time line. IA administration of NF‐κB inhibitor (Bay‐11‐7082 [Sigma; 196870]; 4 μg/kg) and TNFα inhibitor (neutralizing monoclonal antibody [Abcam; ab185795]; 4 μg/kg) were introduced in the same manner as rmSost. Dimethyl sulfoxide (DMSO) and saline were used as controls for NF‐κB and TNFα inhibitor, respectively. Refer Fig. 3 A for time line.
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