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Elh igf1

Manufactured by RayBiotech
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Sourced in United States

Elh-IGF1 is a laboratory product designed to detect and quantify Insulin-like Growth Factor 1 (IGF-1) in biological samples. It is a sensitive and specific assay that utilizes the enzyme-linked immunosorbent assay (ELISA) technique. The core function of Elh-IGF1 is to provide researchers with a reliable tool for measuring IGF-1 levels in various experimental models and clinical studies.

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Lab products found in correlation

Tgf β1, by Enzo Life Sciences (1 mentions)

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ELH-IGF1-1 (2 citations)

2 protocols using elh igf1

1

Quantifying Cellular Growth Factors

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To measure insulin-like growth factor 1 (IGF-1) (Elh-IGF1, RayBiotech, GA, United States), transforming growth factor-β1 (TGF- β1, Enzo Life Science Inc, NY, United States), and dihydrotestosterone (ALPCO, NH, United States) levels in the cell lysate, enzyme-linked immunosorbent assay was used. All reaction and results were calculated after data were obtained with a microplate reader (BMG Biotech, Berlin, GmBH) at a suggested wavelength according to the manufacturer’s instruction.
Ha E.J., Yun J.H., Si C., Bae Y.S., Jeong Y.H., Park K.H, & Choi S.E. (2021). Application of Ethanol Extracts From Alnus sibirica Fisch. ex Turcz in Hair Growth Promotion. Frontiers in Bioengineering and Biotechnology, 9, 673314.
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2

Quantifying Growth Factors in Cell and Tumor Lysates

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VEGF ELISA (DVE00, R&D Systems, Minneapolis, MN), IL8 ELISA (D8000C, R&D System, Minneapolis, MN) and IGF-1 ELISA (ELH-IGF-1, Ray Bio, Norcross, GA) assays were performed according to manufacturer instructions. In brief, SKOv3-DIRAS3 and OVCAR8-DIRAS3 cell lysates, as well as tumor lysates from frozen tissues of isolated xenografts, were collected and triplicate aliquots (100 microliter per well) of lysates with multiple dilutions were loaded onto ELISA plates.. The plates were incubated for 1–2 hrs at RT. After washing, streptavidin-conjugated HRP reagent was added to the wells, and plates were incubated for 30 min at RT. Color development was performed by incubation with HRP substrate and the optical density (O.D.) at 450 nm was determined using a microplate reader.
Mao W., Peters H.L., Sutton M.N., Orozco A.F., Pang L., Yang H., Lu Z, & Bast RC J.r. (2019). The Role of VEGF, IL-8 and IGF in Sustaining Autophagic DIRAS3-induced Dormant Ovarian Cancer Xenografts. Cancer, 125(8), 1267-1280.
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