A total of 2 μg of cell lysate was loaded into each lane of a 10% polyacrylamide gel, then blotted onto a polyvinylidene difluoride (PVDF) membrane. After blocking with PBST containing 5% nonfat dry milk, the membrane was incubated with specific primary antibodies against p-Stat3, Stat3, TLR9, and SOCS3. All antibodies were purchased from Cell Signaling Technologies. Peroxidase-linked IgG (Life Technologies) was used as the secondary antibody. These proteins were visualized with an ECL western blotting detection kit (Amersham Biosciences).
Peroxidase linked igg
Manufactured by Thermo Fisher Scientific
Peroxidase-linked IgG is a type of antibody conjugate that contains a peroxidase enzyme linked to an immunoglobulin G (IgG) molecule. The peroxidase enzyme catalyzes a colorimetric reaction, producing a visible signal when the conjugate binds to a target antigen. This product can be used in various immunoassay and detection techniques.
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Lab products found in correlation
2 protocols using peroxidase linked igg
1
Western Blot Analysis of Signaling Proteins
2
Protein Expression Analysis by Western Blot
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The protein expression levels of p-ERK, p-AKT, p-GSK3β, IRS-1, PTEN and GLTU4 was detected by western blotting. First, 2 μg tissue lysates were loaded on each lane of 10% polyacrylamide gel, and then blotted onto a polyvinylidene difluoride (PVDF) membrane. After blocking with a PBST containing 5% nonfat dry milk, the membrane was incubated with specific primary antibodies (Cell Signaling Technologies, USA). Peroxidase-linked IgG (Life Technologies) were used as secondary antibodies. These proteins were visualized with an ECL western blotting detection kit (Amersham Biosciences).
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