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2 protocols using alexa fluor 647 conjugated goat pab to rabbit igg

1

Immunoblot Analysis of Viral and Cellular Proteins

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The following antibodies were used in this study: p-PLC-γ1(Ser1248) rabbit mAb (cat# 8713), PLC-γ1 pAb (cat# 2822S), β-actin rabbit mAb (cat# 4970), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (cat# 7076), as well as HRP-labeled goat anti-rabbit IgG (cat# 7074) were purchased from Cell Signaling Technology (Danvers, MA, USA). BoHV-1 gD mAb (cat# 1B8-F11) and goat anti-BoHV-1 serum (cat# PAB-IBR) were provided by VMRD Inc. (Pullman, WA, USA). EGFR pAb (cat# A11577) and GOLGA1 rabbit pAb (cat# A14688) were bought from Abclonal Technology (Woburn, MA, USA). GP73/GOLPH2 mouse mAb (cat# 66331-1-lg) was provided by Proteintech (Rosemont, IL, USA). Donkey anti-goat IgG H&L (HRP) (ca# ab97110), Alexa Fluor 647-conjugated goat pAb to rabbit IgG (cat# ab150079), and Alexa Fluor 488-conjugated donkey anti-goat IgG (cat# ab150129) were provided by Abcam (Cambridge, UK). Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) (cat# A-11008) and Alexa Fluor 633-conjugated goat anti-mouse IgG (H + L) (Invitrogen, cat# A-21052) were provided by Invitrogen Life Technologies (Waltham, MA, USA).
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2

Immunoblot Analysis of Viral and Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used in this study: p-PLC-γ1(Ser1248) rabbit mAb (cat# 8713), PLC-γ1 pAb (cat# 2822S), β-actin rabbit mAb (cat# 4970), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (cat# 7076), as well as HRP-labeled goat anti-rabbit IgG (cat# 7074) were purchased from Cell Signaling Technology (Danvers, MA, USA). BoHV-1 gD mAb (cat# 1B8-F11) and goat anti-BoHV-1 serum (cat# PAB-IBR) were provided by VMRD Inc. (Pullman, WA, USA). EGFR pAb (cat# A11577) and GOLGA1 rabbit pAb (cat# A14688) were bought from Abclonal Technology (Woburn, MA, USA). GP73/GOLPH2 mouse mAb (cat# 66331-1-lg) was provided by Proteintech (Rosemont, IL, USA). Donkey anti-goat IgG H&L (HRP) (ca# ab97110), Alexa Fluor 647-conjugated goat pAb to rabbit IgG (cat# ab150079), and Alexa Fluor 488-conjugated donkey anti-goat IgG (cat# ab150129) were provided by Abcam (Cambridge, UK). Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) (cat# A-11008) and Alexa Fluor 633-conjugated goat anti-mouse IgG (H + L) (Invitrogen, cat# A-21052) were provided by Invitrogen Life Technologies (Waltham, MA, USA).
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