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β tubulin e7 s

Manufactured by Developmental Studies Hybridoma Bank
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β-tubulin (E7-s) is a monoclonal antibody that specifically binds to β-tubulin, a subunit of the tubulin protein. Tubulin is a key component of the cytoskeleton and plays a crucial role in cellular processes such as cell division, intracellular transport, and cell motility. The E7-s clone of the β-tubulin antibody is a useful tool for researchers studying the structure and function of the cytoskeleton.

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Lab products found in correlation

Pt52 atf2, by PhosphoSolutions (3 mentions) Ifnb1 ap18065pu n, by OriGene (2 mentions) Uea 1, by Vector Laboratories (1 mentions) Cd45 30 f11, by Thermo Fisher Scientific (1 mentions) β actin ac 74, by Merck Group (1 mentions) Ha antibody, by Fortrea (1 mentions)

4 protocols using β tubulin e7 s

1

Antibody Usage in ATF2 and IFNβ1 Analysis

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Antibodies employed were purchased as follows: ATF2 (C-19 for immunoblotting and C-19X for ChIP assays), PKCε (C15), IFNb1 (E-20, for immunostaining of sections) from Santa Cruz Biotechnology, Inc. (CA, USA); IFNb1 (AP18065PU-N, for intracellular FACS and immunoneutralization assays) from Acris (USA); β-tubulin (E7-s) from Developmental Studies Hybridoma Bank (University of Iowa, USA); pT52-ATF2 (Phosphosolutions, USA). For immunofluorescent staining of ATF2 in patient sections, we used a polyclonal antibody made by SDIX, LLC against the amino acid residues 129-278.
Lau E., Sedy J., Sander C., Shaw M.A., Feng Y., Scortegagna M., Claps G., Robinson S., Cheng P., Srivas R., Soonthornvacharin S., Ideker T., Bosenberg M., Gonzalez R., Robinson W., Chanda S., Ware C., Dummer R., Hoon D., Kirkwood J.M, & Ronai Z.A. (2015). Transcriptional repression of IFNβ1 by ATF2 confers melanoma resistance to therapy. Oncogene, 34(46), 5739-5748.
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2

Antibody Sourcing for Immunoblotting and ChIP

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Antibodies used were purchased as follows: ATF2 (C-19 for immunoblotting and C-19X for ChIP assays), PKCε (C15), and heat shock protein 90α/β (H-114) from Santa Cruz Biotechnology; pT52-ATF2 from PhosphoSolutions; β-tubulin (E7-s) from the Developmental Studies Hybridoma Bank; CD45 (30-F11) from eBiosciences; and NKp46 (29A1.4) from Fisher Scientific. The FITC-conjugated lectins UEA1, LCA, and PSA were purchased from Vector Laboratories. The general membrane stain was purchased from Sigma-Aldrich.
Lau E., Feng Y., Claps G., Fukuda M.N., Perlina A., Donn D., Jilaveanu L., Kluger H., Freeze H.H, & Ronai Z.A. (2015). The transcription factor ATF2 promotes melanoma metastasis by suppressing protein fucosylation. Science signaling, 8(406), ra124.
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3

Antibody Sources and Plasmid Constructs

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The following antibodies were purchased from commercial sources: HA antibody (Covance), GFP antibody (D5.1; Cell Signaling Technology), β-actin (AC-74; Sigma-Aldrich), VP16 (ab4808-100; Abcam), RAS (Ab-3, OP40; EMD Millipore), ERK1 (sc-94, K-23; Santa Cruz Biotechnology, Inc.), and β-tubulin (E7-s; Developmental Studies Hybridoma Bank). GFP-agarose beads (D153-8) were from MBL International. pCXN2-HA-human GPR31 was obtained from J. Miyazaki (RIKEN Center for Developmental Biology, Kobe, Japan; Niwa et al., 1991 (link)). Human GPR31 was inserted into pCGN-HA vector and pEGFP-N1 by standard PCR cloning. Human β2AR was inserted into pEGFP-N1 (β2AR-1) and was also obtained in S65TGFP vector (β2AR-2) from L. Barak (Duke University, Durham, NC). The mouse formyl peptide receptor FPR-rs2 was received in pEGFP-N1 vector from P. Murphy (National Institute of Allergy and Infectious Diseases, Bethesda, MD). The peptide sequence from rat GABA receptor 1b, which contains the ER localization signal RSRR (LLEKENRELEKIIAEKEERVSELRHQLQSRQQLRSRR), was linked to the 3′ end of Flag-GPR31 (human) and cloned into pEGFP-N1. KRAS4B was cloned from pEGFP-C3-KRAS4B into ptdTomato-C1. FTI L-744,832 was purchased from Enzo Life Sciences, and GGTI-2418 was provided by S. Sebti (Moffitt Cancer Center, Tampa, FL).
Fehrenbacher N., Tojal da Silva I., Ramirez C., Zhou Y., Cho K.J., Kuchay S., Shi J., Thomas S., Pagano M., Hancock J.F., Bar-Sagi D, & Philips M.R. (2017). The G protein–coupled receptor GPR31 promotes membrane association of KRAS. The Journal of Cell Biology, 216(8), 2329-2338.
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4

Antibody Usage in ATF2 and IFNβ1 Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies employed were purchased as follows: ATF2 (C-19 for immunoblotting and C-19X for ChIP assays), PKCε (C15), IFNb1 (E-20, for immunostaining of sections) from Santa Cruz Biotechnology, Inc. (CA, USA); IFNb1 (AP18065PU-N, for intracellular FACS and immunoneutralization assays) from Acris (USA); β-tubulin (E7-s) from Developmental Studies Hybridoma Bank (University of Iowa, USA); pT52-ATF2 (Phosphosolutions, USA). For immunofluorescent staining of ATF2 in patient sections, we used a polyclonal antibody made by SDIX, LLC against the amino acid residues 129-278.
Lau E., Sedy J., Sander C., Shaw M.A., Feng Y., Scortegagna M., Claps G., Robinson S., Cheng P., Srivas R., Soonthornvacharin S., Ideker T., Bosenberg M., Gonzalez R., Robinson W., Chanda S., Ware C., Dummer R., Hoon D., Kirkwood J.M, & Ronai Z.A. (2015). Transcriptional repression of IFNβ1 by ATF2 confers melanoma resistance to therapy. Oncogene, 34(46), 5739-5748.
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