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Ab100639 sonic hedgehog human elisa kit

Manufactured by Abcam
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The Ab100639-Sonic Hedgehog Human ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the detection and quantification of human Sonic Hedgehog protein in biological samples.

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Lab products found in correlation

1420 multilabel counter, by PerkinElmer (1 mentions) M30 apoptosense ck18 kit, by Diapharma (1 mentions)

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ELISAs (10 citations) Sonic Hedgehog Human ELISA kit (4 citations) Sonic Hedgehog (3 citations)

2 protocols using ab100639 sonic hedgehog human elisa kit

1

Sonic Hedgehog Protein ELISA Protocol

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SHH ELISA was performed using the ab100639-Sonic Hedgehog Human ELISA Kit (Abcam). Briefly, cell-culture media samples were harvested from 3 individual wells of 12-well plates after culturing for 6, 18, 24, 30, 42, or 54 h and diluted 10-fold by using 1× assay diluent B, then incubated overnight at 4 °C in the ELISA plate. The OD values were measured at 450 nm with a 1420 Multilabel Counter (Perkin Elmer Inc.).
Li H., Liu W., Chen W., Zhu J., Deng C.X, & Rodgers G.P. (2015). Olfactomedin 4 deficiency promotes prostate neoplastic progression and is associated with upregulation of the hedgehog-signaling pathway. Scientific Reports, 5, 16974.
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2

Evaluating Circulating Apoptosis and Hedgehog Markers

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Using fasting serum, circulating analytes [M30 Apoptosense® CK18 Kit (DiaPharma, OH) and M65 EpiDeath® ELISA (DiaPharma, OH)] were measured by ELISA. Circulating SHH concentrations were measured by ab100639 – Sonic Hedgehog Human ELISA Kit (Abcam, MA). All protocols were performed as per manufacturers’ instructions.
Estep M., Mehta R., Bratthauer G., Alaparthi L., Monge F., Ali S., Abdelatif D., Younoszai Z., Stepanova M., Goodman Z.D, & Younossi Z.M. (2019). Hepatic sonic hedgehog protein expression measured by computer assisted morphometry significantly correlates with features of non-alcoholic steatohepatitis. BMC Gastroenterology, 19, 27.
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