RPMI 1640 medium and fetal bovine serum were from Gibco. Amphotericin B was from Invitrogen Life Technologies. Resazurin salt, propidium iodide, and RNase were from Sigma Aldrich. Annexin V/7-amino-actinomycin D (7-AAD) apoptosis detection kit was obtained from BD Bioscience. LDH cytotoxicity assay kit was from Roche. Fast staining kit (RAL-555) was from RAL Reactifs. All other reagents are of analytical grade from different commercial sources.
Annexin v 7 amino actinomycin d 7 aad apoptosis detection kit
Manufactured by BD
Sourced in United States
The Annexin V/7-amino-actinomycin D (7-AAD) apoptosis detection kit is a laboratory assay used to detect and quantify apoptosis, a form of programmed cell death. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and 7-AAD, a dye that stains DNA, to distinguish between viable, apoptotic, and necrotic cells.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Resazurin salt, by Merck Group (1 mentions)
Ldh cytotoxicity assay kit, by Roche (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Amphotericin b, by Thermo Fisher Scientific (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Facsaria, by BD (1 mentions)
High glucose dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Antibodies against cleaved caspase 3, by Proteintech (1 mentions)
Cct020312, by MedChemExpress (1 mentions)
Ex527, by MedChemExpress (1 mentions)
Cell counting kit 8 cck 8, by Biosharp (1 mentions)
4 protocols using annexin v 7 amino actinomycin d 7 aad apoptosis detection kit
1
Cell Viability and Cytotoxicity Assay
2
Apoptosis Quantification via Annexin V/7-AAD
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Cell apoptosis was measured using the Annexin V/7-Aminoactinomycin D (7-AAD) Apoptosis Detection kit (BD Biosciences, San Diego, CA, USA) according to the manufacturer's protocol. Cell suspensions were passed through 40-μm filter caps to remove possible cell clumps. After washing in PBS, cells were resuspended in 1× binding buffer. Annexin V and 7-AAD were added, and cells were incubated at room temperature for 15 minutes in dark. Stained cells were analyzed immediately using a flow cytometer (FACSAria; BD Biosciences).
3
Annexin V/7-AAD Apoptosis Assay
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For the detection of apoptotic or necrotic cell death, the annexin V/7-amino-actinomycin D (7-AAD) apoptosis detection kit (BD Bioscience) was used according to the manufacturer's protocol. After 24 and 72 h incubation with essential oils, promastigotes (106 cells/mL) were washed in phosphate-buffered saline (PBS) and centrifuged at 1800 rpm (630 g) for 10 min. Then, they were stained with annexin V-PE/7-AAD for 15 min in the dark at room temperature [37 (link)]. Acquisition was performed using a BD Biosciences flow cytometer. Data were analyzed using the CellQuest software and the percentage of positive cells was determined for each sample.
4
Investigating PC12 Cell Apoptosis
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PC12 rat adrenal pheochromocytoma cells were obtained from Icell Bioscience Inc., Shanghai. BUP hydrochloride and RSV were purchased from Sigma-Aldrich (Merck KGaA), while CCT020312 and EX527 were obtained from MedChemExpress. High-glucose Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco (Thermo Fisher Scientific, Inc.). The Cell Counting Kit-8 (CCK-8) was supplied by Biosharp Life Sciences, and the Annexin V/7-aminoactinomycin D (7-AAD) apoptosis detection kit was provided by BD Biosciences. Antibodies against SIRT1 (cat. no. ab110304) and caspase-12 (cat. no. ab62484) were purchased from Abcam; antibodies against PERK (cat. no. 3192S), phosphorylated (p)-eIF2α (cat. no. 3398T), eIF2α (cat. no. 5324T), CHOP (cat. no. 5554T) and ATF4 (cat. no. 11815) were supplied by Cell Signaling Technology, Inc.; antibodies against Bax (cat. no. 60267-1-Ig), Bcl-2 (cat. no. 26593-1-AP), glucose-regulated protein 78 (GRP78; cat. no. 11587-1-AP) and GAPDH (cat. no 10494-1-AP) were provided by Proteintech Group, Inc.; and antibodies against cleaved caspase-3 (cat. no. WL02117) and p-PERK (cat. no. WL05295) were obtained from Wanleibio Co., Ltd.
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