Human cytokine panel 1

Manufactured by Merck Group

The Human Cytokine Panel I is a multiplex assay designed to detect and quantify the levels of multiple human cytokines simultaneously in a single sample. It provides a comprehensive assessment of the immune system's signaling molecules. The panel measures the concentrations of various cytokines using a validated, highly sensitive, and reproducible platform.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions)

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Human Cytokine/Chemokine Panel I (8 citations) Human Cytokine/Chemokine Panel I (MPXHCYTO-60K; (5 citations) Milliplex MAP Human Cytokine/Chemokine panel 1 (3 citations) Human Cytokine/Chemokine Panel I Multiplex Array (3 citations) MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel 1-Premixed 38 Plex (2 citations) Milliplex Human Cytokine/Chemokine Panel I (2 citations) MILLIPLEX MAP multiplex Human Cytokine Panel 1 (2 citations) Milliplex 41-plex human cytokine panel 1 (2 citations)

2 protocols using human cytokine panel 1

Airway Immune Profile in Asymptomatic Smokers

Cited 2 times

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Between 2010 and 2013, bronchoalveolar lavage (BAL) fluid was collected from 30 asymptomatic subjects (10 nonsmokers, 14 former smokers, and 6 current smokers) as part of previous studies evaluating the lower airway bacteriome and inflammation [29 (link), 30 (link)]. Briefly, bronchoscopy was performed via nasal approach and avoiding suctioning until the scope was positioned for sampling. Sequential BAL was collected from the lingula and right middle lobe, combined, and processed. Metabolites and cytokine numbers were measured as previously described [29 (link), 30 (link)], and identified metabolites were reported if present in ≥50% of the samples. Intensity data were mean-centered and divided by the standard deviation using MetaboAnalyst [31 (link)]. For in vivo cytokines, 39 cytokines were measured with a Luminex 200IS (Luminext Corp, Austin, TX) using Human Cytokine Panel I (Millipore, Billerica, MA). Data were analyzed with MasterPlex TM QT software (version 1–2, MiraiBio, Inc. Alameda, CA).

Gregory A.C., Sullivan M.B., Segal L.N, & Keller B.C. (2018). Smoking is associated with quantifiable differences in the human lung DNA virome and metabolome. Respiratory Research, 19, 174.

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Alveolar Macrophage Cytokine Profiling

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In a subset of samples from the NYU cohort (n = 18), alveolar macrophages were isolated for ex vivo TLR4 stimulation. BAL macrophages were isolated by plastic adhesion and cells (2 × 10⁶) were incubated in 12-well plates with RPMI (Gibco), plus 10% fetal calf serum (HyClone) and 2% penicillin-streptomycin (Gibco) with or without 40 ng LPS ml⁻¹ (Escherichia coli 0.55:B4 and B5, Sigma-Aldrich). After 24 h, supernatants were collected and assayed using Human Cytokine Panel I (Millipore) according to the manufacturer’s instructions in a Luminex 200IS system (Luminex). Data were analysed with MasterPlex TM QT software (version 1–2, MiraiBio). Average results from technical duplicates were utilized.

Segal L.N., Clemente J.C., Tsay J.C., Koralov S.B., Keller B.C., Wu B.G., Li Y., Shen N., Ghedin E., Morris A., Diaz P., Huang L., Wikoff W.R., Ubeda C., Artacho A., Rom W.N., Sterman D.H., Collman R.G., Blaser M.J, & Weiden M.D. (2016). Enrichment of the lung microbiome with oral taxa is associated with lung inflammation of a Th17 phenotype. Nature microbiology, 1, 16031.

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