The human PCa cell lines 22RV1, PC-3, VCaP, DU145, LNCaP and normal prostate epithelial cells RWPE-1 were obtained from Shanghai Chinese Academy of Sciences cell bank (China). RWPE-1 cells were grown in defined keratinocyte-SFM (1×) (Invitrogen). PC-3, LNCaP and 22Rv1 cells were cultured in RPMI-1640 medium (Life Technologies, Carlsbad, CA, US) supplemented with penicillin G (100 U/ml), streptomycin (100 mg/ml) and 10% fetal bovine serum (FBS, Life Technologies). DU145 and VCaP cells were grown in Dulbecco’s modified Eagle’s medium (Invitrogen) supplemented with 10% FBS. The C4-2B cell line was purchased from the MD Anderson Cancer Center and maintained in T-medium (Invitrogen) supplemented with 10% FBS [31 (link)]. All cell lines were grown under a humidified atmosphere of 5% CO2 at 37 °C. A hypoxic condition was induced via culturing the cells under 1% oxygen tension (1% O2) in a hypoxia chamber for 24–48 h, as previously described [32 (link)], as well as treated the cells with 50–200 μmol L−1 cobalt chloride (CoCl2) for 24 h to mimic the hypoxic condition by stabilization of HIF-1a [33 (link)].
Defined keratinocyte sfm 1
Manufactured by Thermo Fisher Scientific
Sourced in United States
Defined keratinocyte-SFM (1×) is a cell culture medium formulation designed for the growth and maintenance of human keratinocytes in a serum-free environment. It contains essential nutrients, growth factors, and supplements required for the optimal proliferation and differentiation of keratinocytes.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (7 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (7 mentions)
T medium, by Thermo Fisher Scientific (7 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
C4 2b cell line, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Skov3, by American Type Culture Collection (1 mentions)
Tov 21g, by American Type Culture Collection (1 mentions)
Tov112d, by American Type Culture Collection (1 mentions)
Hosepic, by American Type Culture Collection (1 mentions)
Ovcar4, by American Type Culture Collection (1 mentions)
A2780, by American Type Culture Collection (1 mentions)
Cov362, by American Type Culture Collection (1 mentions)
Cov644, by American Type Culture Collection (1 mentions)
Cov434, by American Type Culture Collection (1 mentions)
8 protocols using defined keratinocyte sfm 1
1
Culturing and Hypoxia Treatment of Prostate Cell Lines
2
Culturing Prostate Cell Lines
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Human PCa cell lines 22RV1, PC-3, VCaP, DU145, LNCaP and normal prostate epithelial cells RWPE-1 were obtained from the Shanghai Chinese Academy of Sciences cell bank (China). RWPE-1 cells were grown in defined keratinocyte-SFM (1×) (Invitrogen). PC-3, LNCaP and 22Rv1 cells were cultured in RPMI-1640 medium (Life Technologies, Carlsbad, CA, US) supplemented with penicillin G (100 U/ml), streptomycin (100 mg/ml) and 10% fetal bovine serum (FBS, Life Technologies). DU145 and VCaP cells were grown in Dulbecco's modified Eagle's medium (Invitrogen) supplemented with 10% FBS. The C4-2B cell line was purchased from the MD Anderson Cancer Center and maintained in T-medium (Invitrogen) supplemented with 10 % FBS. All cell lines were grown under a humidified atmosphere of 5 % CO2 at 37 °C.
3
Prostate Cancer Cell Line Cultivation
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The human PCa cell lines 22RV1, PC-3, VCaP, DU145, LNCaP, and normal prostate epithelial cells RWPE-1 were obtained from Shanghai Chinese Academy of Sciences cell bank (China). RWPE-1 cells were grown in defined keratinocyte-SFM (1×) (Invitrogen). PC-3, LNCaP, and 22Rv1 cells were cultured in RPMI-1640 medium (Life Technologies, Carlsbad, CA, US) supplemented with penicillin G (100 U/mL), streptomycin (100 mg/mL), and 10% fetal bovine serum (FBS, Life Technologies). DU145 and VCaP cells were grown in DMEM (Invitrogen) supplemented with 10% FBS. The C4-2B cell line was purchased from the MD Anderson Cancer Center and maintained in T-medium (Invitrogen) supplemented with 10% FBS. All cell lines were grown under a humidified atmosphere of 5% CO2 at 37°C. All cell lines were authenticated by short tandem repeat fingerprinting at Guangzhou Cellcook Biotech on June 19, 2017.
4
Culturing Human Prostate Cell Lines
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The human PCa cell lines 22RV1, PC-3, VCaP, DU145, LNCaP and normal prostate epithelial cells RWPE-1 were obtained from Shanghai Chinese Academy of Sciences cell bank (China). RWPE-1 cells were grown in defined keratinocyte-SFM (1×) (Invitrogen). PC-3, LNCaP and 22Rv1 cells were cultured in RPMI-1640 medium (Life Technologies, Carlsbad, CA, US) supplemented with penicillin G (100 U/ml), streptomycin (100 mg/ml) and 10% fetal bovine serum (FBS, Life Technologies). DU145 and VCaP cells were grown in Dulbecco’s modified Eagle’s medium (Invitrogen) supplemented with 10% FBS. The C4-2B cell line was purchased from the MD Anderson Cancer Center and maintained in T-medium (Invitrogen) supplemented with 10 % FBS. All cell lines were grown under a humidified atmosphere of 5% CO2 at 37 °C.
5
Prostate Cancer Cell Culture Protocols
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The human PCa cell lines 22RV1, PC-3, VCaP, DU145, LNCaP and normal prostate epithelial cells RWPE-1 were obtained from Shanghai Chinese Academy of Sciences cell bank (China). RWPE-1 cells were grown in defined keratinocyte-SFM (1×) (Invitrogen). PC-3, LNCaP and 22Rv1 cells were cultured in RPMI-1640 medium (Life Technologies, Carlsbad, CA, US) supplemented with penicillin G (100 U/ml), streptomycin (100 mg/ml) and 10% fetal bovine serum (FBS, Life Technologies). DU145 and VCaP cells were grown in Dulbecco’s modified Eagle’s medium (Invitrogen) supplemented with 10% FBS. The C4-2B cell line was purchased from the MD Anderson Cancer Center and maintained in T-medium (Invitrogen) supplemented with 10% FBS. All cell lines were grown under a humidified atmosphere of 5% CO2 at 37 °C.
6
Prostate Cancer Cell Culture Protocols
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The human PCa cell lines 22RV1, PC-3, VCaP, DU145, LNCaP and normal prostate epithelial cells RWPE-1 were obtained from Shanghai Chinese Academy of Sciences cell bank (China). RWPE-1 cells were grown in defined keratinocyte-SFM (1×) (Invitrogen). PC-3, LNCaP and 22Rv1 cells were cultured in RPMI-1640 medium (Life Technologies, Carlsbad, CA, US) supplemented with penicillin G (100 U/ml), streptomycin (100 mg/ml) and 10% fetal bovine serum (FBS, Life Technologies). DU145 and VCaP cells were grown in Dulbecco’s modified Eagle’s medium (Invitrogen) supplemented with 10% FBS. The C4-2B cell line was purchased from the MD Anderson Cancer Center and maintained in T-medium (Invitrogen) supplemented with 10% FBS. All cell lines were grown under a humidified atmosphere of 5% CO2 at 37 °C.
7
Culturing Ovarian Cancer Cell Lines
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The human ovarian cancer cell lines (COV644, COV362, OV90, SKOV3, TOV112D, OVCAR4, A2780, and COV434, TOV21G) and normal ovarian epithelial cells (HOSEpiC) were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). HOSEpiC were cultured in Defined Keratinocyte-SFM (1×) (Invitrogen, Carlsbad, CA, USA). The ovarian cancer cell lines were cultured in Dulbecco’s modified Eagle’s medium (Invitrogen) with 10% fetal bovine serum (Gibco, Grand-Island, NY, USA) and 1% penicillin (100 U/mL) and streptomycin (100 µg/mL) at 37°C under 5% CO2 incubator added.
8
Prostate Cell Lines for Cancer Research
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The human PCa cell lines 22RV1, PC-3, VCaP, DU145, and LNCaP and normal prostate epithelial cells RWPE-1 were obtained from the Shanghai Chinese Academy of Sciences cell bank (China). RWPE-1 cells were grown in Defined Keratinocyte-SFM (1 ×) (Invitrogen, USA). PC-3, LNCaP and 22Rv1 cells were cultured in RPMI-1640 medium (Life Technologies, USA) supplemented with penicillin G (100 U ml–1), streptomycin (100 mg ml–1) and 10% fetal bovine serum (FBS, Life Technologies, USA). DU145 and VCaP cells were grown in Dulbecco's modified Eagle’s medium (Invitrogen, USA) supplemented with 10% FBS. The C4-2B cell line was purchased from MD Anderson Cancer Center and maintained in T-medium (Invitrogen, USA) supplemented with 10% FBS (Wu et al, 1998 (link)). All cell lines were authenticated by short-tandem repeat (STR) fingerprinting at Medicine Lab of Forensic Medicine Department of Sun Yat-Sen University (China). All cell lines were grown under a humidified atmosphere of 5% CO2 at 37 °C.
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