Flag tag for oas1 p46 g

Manufactured by GenScript

The Flag-tag for OAS1-p46-G is a laboratory tool used to facilitate the detection and purification of the OAS1-p46-G protein. The Flag-tag is a short peptide sequence that can be attached to the target protein, allowing it to be identified and isolated using specific antibodies or affinity-based techniques.

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Lab products found in correlation

Oas1 p42 a, by GenScript (2 mentions) Quikchange 2 site directed mutagenesis kit, by Agilent Technologies (2 mentions) Psicheck 2 plasmid, by Promega (1 mentions)

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FLAG (6 citations)

2 protocols using flag tag for oas1 p46 g

Generation of Allele-Specific OAS1 and OAS3 Plasmids

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Plasmids with a Flag-tag for OAS1-p46-G (ID: OHu21619D, includes rs1131454-G, rs1131476-G, and rs1051042-G alleles) and OAS1-p42-A (ID: OHu29197D, includes rs1131454-A allele) were purchased from GenScript. The QuikChange II site-directed mutagenesis kit (Agilent) was used to generate plasmids OAS1-p42-G (rs1131454-G allele) and OAS1-p46-A (rs1131454-A, rs1131476-A and rs1051042-C alleles) using mutagenesis primers (Supplementary Table 12). Additionally, the Kozak sequence of Renilla (hRLuc) of psiCHECK-2 plasmid (Promega) was mutated to generate allele-specific plasmids for rs1859331-C and rs1859331-A within 5′ UTR of OAS3. The original and modified plasmids were confirmed by Sanger sequencing.

Banday A.R., Stanifer M.L., Florez-Vargas O., Onabajo O.O., Papenberg B.W., Zahoor M.A., Mirabello L., Ring T.J., Lee C.H., Albert P.S., Andreakos E., Arons E., Barsh G., Biesecker L.G., Boyle D.L., Brahier M.S., Burnett-Hartman A., Carrington M., Chang E., Choe P.G., Chisholm R.L., Colli L.M., Dalgard C.L., Dude C.M., Edberg J., Erdmann N., Feigelson H.S., Fonseca B.A., Firestein G.S., Gehring A.J., Guo C., Ho M., Holland S., Hutchinson A.A., Im H., Irby L., Ison M.G., Joseph N.T., Kim H.B., Kreitman R.J., Korf B.R., Lipkin S.M., Mahgoub S.M., Mohammed I., Paschoalini G.L., Pacheco J.A., Peluso M.J., Rader D.J., Redden D.T., Ritchie M.D., Rosenblum B., Ross M.E., Anna H.P., Savage S.A., Sharma S., Siouti E., Smith A.K., Triantafyllia V., Vargas J.M., Vargas J.D., Verma A., Vij V., Wesemann D.R., Yeager M., Yu X., Zhang Y., Boulant S., Chanock S.J., Feld J.J, & Prokunina-Olsson L. (2022). Genetic regulation of OAS1 nonsense-mediated decay underlies association with COVID-19 hospitalization in patients of European and African ancestries. Nature Genetics, 54(8), 1103-1116.

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Generation of OAS1 Plasmid Variants

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Plasmids with a FLAG-tag for OAS1-p46-G (ID: OHu21619D, rs1131454-G, rs1131476-G, rs1051042-G) and OAS1-p42-A (ID: OHu29197D, rs1131454-A) were purchased from GenScript. QuikChange II site-directed mutagenesis kit (Agilent 200523) was used to generate plasmids OAS1-p42-G (rs1131454-G) and OAS1-p46-A (rs1131454-A, rs1131476-A, rs1051042-C) using mutagenesis primers (Table S14). Sequences of original and modified plasmids were confirmed by Sanger sequencing.

Banday A.R., Stanifer M.L., Florez-Vargas O., Onabajo O.O., Zahoor M.A., Papenberg B.W., Ring T.J., Lee C.H., Andreakos E., Arons E., Barsh G., Biesecker L.G., Boyle D.L., Burnett-Hartman A., Carrington M., Chang E., Choe P.G., Chrisholm R.L., Dalgard C., Edberg J., Erdmann N., Feigelson H.S., Firestein G.S., Gehring A.J., Ho M., Holland S., Hutchinson A.A., Im H., Ison M.G., Kim H.B., Kreitman R.J., Korf B.R., Mirabello L., Pacheco J.A., Peluso M.J., Rader D.J., Redden D.T., Ritchie M.D., Rosenbloom B., Sant Anna H.P., Savage S., Siouti E., Triantafyllia V., Vargas J.M., Verma A., Vij V., Wesemann D.R., Yeager M., Yu X., Zhang Y., Boulant S., Chanock S.J., Feld J.J, & Prokunina-Olsson L. (2021). Genetic regulation of OAS1 nonsense-mediated decay underlies association with risk of severe COVID-19. medRxiv.

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