Nylon cell strainer

Manufactured by Avantor

Sourced in United States

The Nylon cell strainer is a laboratory equipment designed for the filtration and separation of cells, tissues, or other particulate matter. It features a nylon mesh that is durable and efficient in trapping the desired sample while allowing the liquid to pass through.

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Lab products found in correlation

Collagenase, by Merck Group (2 mentions) Chromabond, by Macherey-Nagel (1 mentions) Gibco ack lysis buffer, by Thermo Fisher Scientific (1 mentions) Isotonic percoll, by GE Healthcare (1 mentions) Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions) Trypan blue, by Avantor (1 mentions) Fetal calf serum (fcs), by Avantor (1 mentions) B rker chamber, by Avantor (1 mentions) Dnase 1, by Merck Group (1 mentions) Percoll solution, by Merck Group (1 mentions) Dulbecco s phosphate buffered saline dpbs, by Merck Group (1 mentions)

Spelling variants of this product

Cell strainer (15 citations) 70μm nylon cell strainer (2 citations)

7 protocols using nylon cell strainer

Preparation of Rhizophagus irregularis Spore Exudates

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Rhizophagus irregularis DAOM 197198 sterile spores were purchased from Agronutrition (Labège, France). The spores were produced in axenic conditions. Spore numbers were determined by the supplier by counting spores in an aliquot of the sold suspension with a binocular microscope. Spores were rinsed from their storage buffer using a 40 μm nylon cell strainer (VWR) by five washes with sterile UHQ water. Spores were resuspended in sterile UHQ water and stored at 4°C before use.
For the production of germinated spore exudates (GSE), spores were germinated in sterile UHQ water in a CO₂ incubator (30°C, 2% CO₂) for 7 days with a concentration of 400 spores.mL^-1 in 25 mL Petri dishes. GSE were filtered through a glass-fiber frit (Chromabond, Macherey-Nagel, France), then frozen in liquid nitrogen and freeze-dried. Filtered spores were collected and stored at -80°C.

Pons S., Fournier S., Chervin C., Bécard G., Rochange S., Frei Dit Frey N, & Puech Pagès V. (2020). Phytohormone production by the arbuscular mycorrhizal fungus Rhizophagus irregularis. PLoS ONE, 15(10), e0240886.

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Mouse Spleen Cell Isolation

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The spleen was removed from each mouse using forceps and placed in a small petri dish containing 10 ml of PBS pH 7.4 with 0.38% sodium citrate. Spleens were mashed through a 70 μm Nylon cell strainer (VWR, Swedesboro, NJ, USA) with the blunt end of a 3 ml syringe. The cell strainer was rinsed with 2.5 ml of PBS pH 7.4 containing 0.38% sodium citrate. The mashed spleen was centrifuged at 4°C, 800 x g for 8 minutes. The supernatant was discarded and 5 ml of Gibco ACK Lysis Buffer (Thermo Fisher Scientific, Waltham, MA, USA) was added to the pellet, resuspended, and incubated for 5 min. 30 ml of cold PBS was added to the resuspended cells, mixed gently, and centrifuged at 4°C, 800 x g for 8 min. The supernatant was removed, and pellet resuspended in 2% FCS in PBS pH 7.4 and used for flow cytometric analysis.

Walker J.M., Sundarasivarao P.Y., Thornton J.M., Sochacki K., Rodriguez A., Spur B.W., Acharya N.K, & Yin K. (2022). Resolvin D2 promotes host defense in a 2 - hit model of sepsis with secondary lung infection. Prostaglandins & other lipid mediators, 159, 106617.

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Isolating Microglia from Rat Brain

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Microglia were isolated from brain tissue by Percoll gradient centrifugation as described previously (Frank, Wieseler-Frank, Watkins, & Maier, 2006 (link)), with slight modification. Based on the time course of microglia activation in this model of an AUD, rats were humanely killed at 0, 2, 4, and 7 days following the last dose of ethanol (i.e., T0, T2, T4, and T7): rats were deeply anesthetized and transcardially perfused with 0.9% NaCl containing heparin. Brains were harvested and the hippocampus and entorhinal cortex were dissected on ice. Tissue was finely minced with a razor blade and gently homogenized in Dulbecco’s phosphate-buffered saline (DPBS), pH 7.4, then passed through a 70-μm nylon cell strainer (VWR, Batavia, IL). Resulting homogenates were centrifuged at 400 × g for 6 min and cell pellets were resuspended in 2 mL 50% isotonic Percoll (GE Healthcare, Piscataway, NJ). Two milliliters of 50% isotonic Percoll was gently layered on top of 1 mL 70% layer and then 1 mL 1× PBS was layered on top of the 50% Percoll layer. The density gradient was centrifuged at 1200 × g for 45 min (minimum acceleration and brake) at 20 °C. Microglia were collected from the interphase between the 70% and 50% isotonic Percoll phases (Frank et al., 2006 (link)). Cells were washed in 1× PBS and then resuspended in sterile DPBS.

Peng H., Nickell C.R., Chen K.Y., McClain J.A, & Nixon K. (2017). Increased expression of M1 and M2 phenotypic markers in isolated microglia after four-day binge alcohol exposure in male rats. Alcohol (Fayetteville, N.Y.), 62, 29-40.

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Spleen Dissection and Cell Isolation

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During dissection, spleens were removed and collected in phosphate buffered saline (PBS) + 2% fetal calf serum (FCS, Gibco) at 4°C. Single cells were obtained after mashing the spleen through a 70 µm nylon cell strainer (VWR) followed by treatment with a RBC lysis buffer (0.83% ammonium chloride (NH₄Cl; Acros Organics, Geel, Belgium)/10 mM Tris (Sigma) solution with pH 7.2) at 37°C. After washing with PBS + 2% FCS, cells were resuspended in PBS + 2% FCS and live leukocytes were counted in a Bürker chamber after 1/2 dilution in trypan blue (VWR).

Pollenus E., Pham T.T., Vandermosten L., Robalo Q., Possemiers H., Knoops S., Opdenakker G, & Van den Steen P.E. (2021). CCR2 Is Dispensable for Disease Resolution but Required for the Restoration of Leukocyte Homeostasis Upon Experimental Malaria-Associated Acute Respiratory Distress Syndrome. Frontiers in Immunology, 11, 628643.

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Isolation of Tumor and Immune Cells

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MC38-CEA2 and MC38-CEA2-GFP tumor tissues were harvested, weighed and minced into small pieces. Samples were suspended in RPMI 1640 containing collagenase (1 mg/mL; Sigma-Aldrich) and DNase I (100 μg/mL; Sigma-Aldrich) and transferred to MACS tubes and further disrupted on a gentle MACS dissociator (program mTumor-01). Samples were incubated for 30 min at 37°C, then filtered through a 70 μm nylon cell strainer (VWR) and pelleted by centrifugation. Pelleted tumor digest was suspended in 35% percoll solution (Sigma-Aldrich) and centrifuged at 1,600 rpm for 7 min to remove extra fatty tissue. Spleen and lymph node tissues were meshed and filtered through a 40 μM cell strainer and red blood cells lysed. Cells were counted and suspended in PBS supplemented with 0.5% BSA for flow cytometry staining.

Guan Y., Kraus S.G., Quaney M.J., Daniels M.A., Mitchem J.B, & Teixeiro E. (2020). FOLFOX Chemotherapy Ameliorates CD8 T Lymphocyte Exhaustion and Enhances Checkpoint Blockade Efficacy in Colorectal Cancer. Frontiers in Oncology, 10, 586.

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Subcutaneous EL-4 Lymphoma Xenograft Assay

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2 × 10⁵ EL-4 cells were suspended in 0.1 ml 1X PBS (Intron). The cells were subcutaneously injected into mice as previously described [29 (link)], and tumor growth was monitored by measuring the tumor volumes (0.5 × (length × width²)) with calipers. After 3 weeks of injection, EL-4 lymphoma cells were extracted, weighed, and then digested into single cells. Three independent experiments were performed with at least three mice per group. Single cells were harvested using a 70 μm nylon cell strainer (VWR) and analyzed using flow cytometry (FACS CantoII) at the Core Facility Center for Chronic and Metabolic Diseases at Sookmyung Women’s University.

Soh S., Han S., Ka H.I., Mun S.H., Kim W., Oh G, & Yang Y. (2023). Adiponectin affects the migration ability of bone marrow-derived mesenchymal stem cells via the regulation of hypoxia inducible factor 1α. Cell Communication and Signaling : CCS, 21, 158.

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Isolation of Primary Ovarian Cancer Cells

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Tumor and blood samples were obtained from 27 patients with EOC, 4 patients with metastatic cancer, and 8 patients with benign tumors. In addition, fallopian tube tissue samples were obtained from 10 females diagnosed with EOC and 3 women with metastatic cancers. Tumor and fallopian tube tissue samples were collected at the time of surgery in ice-cold Dulbecco’s Phosphate Buffered Saline (DPBS; Sigma-Aldrich Co. Ltd., Ayrshire, UK) and processed within 1 hour. The solid ovarian cancer specimens were washed with ice-cold DPBS to remove contaminating blood and then minced into small pieces (approximately 1–2 mm³). Primary ovarian cancer cells were isolated from solid specimens using the modified method described by Pribyl et al.⁷⁷. The tissue pieces were digested with 150 U/mL collagenase (Sigma-Aldrich, St. Louis, MO, USA) for 30 min at 37 °C and then transferred onto a nylon cell strainer (100 µm; VWR International, Radnor, PA) to separate EOC cells. The cultures were maintained at 37 °C in 5% CO₂ for several days. EOC cells form a confluent monolayer after two weeks in culture. The cells were collected by centrifugation and stored at −80 °C until use.

Paradowska E., Jabłońska A., Studzińska M., Wilczyński M, & Wilczyński J.R. (2019). Detection and genotyping of CMV and HPV in tumors and fallopian tubes from epithelial ovarian cancer patients. Scientific Reports, 9, 19935.

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