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μct40 scanner

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The μCT40 scanner is a compact and high-resolution micro-computed tomography (micro-CT) system designed for non-destructive imaging and analysis of small samples. The system utilizes X-ray technology to generate detailed three-dimensional images of the internal structure and composition of a wide range of materials and samples.

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Lab products found in correlation

Evaluation software, by Scanco (2 mentions) Factory supplied phantom, by Scanco (2 mentions) Microview, by GE Healthcare (1 mentions) Osmium tetroxide, by Polysciences (1 mentions) Metamorph imaging system, by Universal Imaging (1 mentions) Matrigel, by BD (1 mentions) Green fluorescent calcein, by Merck Group (1 mentions) Q500mc, by Leica camera (1 mentions) Vivact 40, by Scanco (1 mentions) Sigmaplot software, by Grafiti LLC (1 mentions) Software, by Scanco (1 mentions) Osmium tetroxide, by Electron Microscopy Sciences (1 mentions) Osteomeasure analysis system, by OsteoMetrics (1 mentions) Piximus 2 bone densitometer, by GE Healthcare (1 mentions) Calcein, by Merck Group (1 mentions)

60 protocols using μct40 scanner

1

Micro-CT Analysis of Murine Femurs

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Micro-computed tomography (μCT) was done on the femurs of 20-week-old male and female mice or 1-year-old male mice. Bones were fixed in 10% neutral buffered formalin and then embedded in 2% agarose gel. The trabecular volume of the distal femoral metaphysis was measured using a Scanco μCT40 scanner (Scanco Medical AG, Bassersdorf, Switzerland), calibrated using a hydroxyapatite phantom. A threshold of 250 was used for evaluation for all male scans, and a threshold of 212 was used for evaluation for all female scans. Fifty slices were analyzed, starting with the first slice in which condyles and primary spongiosa were no longer visible. Trabecular bone was contoured to exclude the cortical bone, allowing cancellous bone volume/tissue volume (BV/TV) and BMD to be determined. vBMD was measured as mean values of everything within the volume of interest (mixture of bone and background). Since the scan was calibrated for bone, the mean value was represented in units of Hydroxyapatite density [mg HA/ccm].
Rohatgi N., Zou W., Li Y., Cho K., Collins P.L., Tycksen E., Pandey G., DeSelm C.J., Patti G.J., Dey A, & Teitelbaum S.L. (2023). BAP1 promotes osteoclast function by metabolic reprogramming. Nature Communications, 14, 5923.
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2

Micro-CT Analysis of Bone in Mice

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Following DXA and pQCT scans, mice were sacrificed using CO2 and the left femora and 5th lumbar vertebrae (LV5) were removed and fixed in 10% neutral buffered formalin overnight. Formalin fixed bones were transferred to 70% ethanol and stored at 4°C until time of analysis. Micro-CT was used for nondestructive three-dimensional evaluation of bone volume and cortical and cancellous bone architecture. Total femora and total LV5 were scanned at a voxel size of 12 × 12 × 12 μm using a Scanco μCT40 scanner (Scanco Medical AG, Brüttisellen, Switzerland). The threshold for analysis was determined empirically and set at 245 (0-1,000 range). Total femur bone volume was determined followed by evaluation of 20 slices (0.32 mm) of cortical bone in the femoral midshaft. Direct midshaft cortical measurements included cross-sectional volume (volume of cortical bone and bone marrow, mm3), cortical volume (mm3), marrow volume (mm3), cortical thickness (μm), and polar moment of inertia (IPolar). Total bone volume was also determined for LV5. This was followed by evaluation of cancellous bone (entire region of secondary spongiosa between the cranial and caudal growth plates) in the vertebral body. Cancellous bone measurements included bone volume/tissue volume (%), trabecular number (mm-1), trabecular thickness (μm), and trabecular spacing (μm).
Hawse J.R., Pitel K.S., Cicek M., Philbrick K.A., Gingery A., Peters K.D., Syed F.A., Ingle J.N., Suman V.J., Iwaniec U.T., Turner R.T., Spelsberg T.C, & Subramaniam M. (2014). TGFβ Inducible Early Gene-1 Plays an Important Role in Mediating Estrogen Signaling in the Skeleton. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 29(5), 1206-1216.
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3

Micro-CT Analysis of Femur and Vertebra

Cited 1 time
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Micro-CT was performed on the left femora and 3rd lumbar vertebrae (LV3) of each mouse following overnight fixation with 10% neutral buffered formalin. μCT was completed as previously described (Lee et al., 2017 (link); Philbrick et al., 2015 (link)). Briefly, femora were scanned using a Scanco μCT40 scanner (Scanco Medical AG, Basserdorf, Switzerland) at a voxel size of 12 × 12 × 12 μm (55 kVp x-ray voltage, 145 μA intensity, and 200 ms integration time). Total femora (cancellous + cortical bone) were evaluated followed by analysis of cortical bone in the mid femur diaphysis and cancellous bone in the distal femur metaphysis. For the femoral diaphysis, 20 consecutive slices (240 μm) of bone were evaluated and cross-sectional volume (cortical and marrow volume, mm3), cortical volume (mm3), marrow volume (mm3), and cortical thickness (μm) were measured. For the femoral metaphysis, 42 consecutive slices (504 μm) of cancellous bone, and 75 slices (1,050 μm) proximal to the growth plate, were evaluated. For the vertebra, the region of interest was located between the cranial and caudal growth plates (151 ± 2 slices, 1,812 ± 24 μm). Direct cancellous bone measurements included cancellous bone volume fraction (bone volume/tissue volume, BV/TV, %), connectivity density (mm−3), trabecular number (mm−1), trabecular thickness (μm) and trabecular spacing (μm).
Gingery A., Subramaniam M., Pitel K.S., Li X., Ke H.Z., Turner R.T., Iwaniec U.T, & Hawse J.R. (2020). Sclerostin Antibody Treatment Rescues the Osteopenic Bone Phenotype of TGFß Inducible Early Gene-1 Knockout Female Mice. Journal of cellular physiology, 235(7-8), 5679-5688.
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4

Micro-CT Analysis of Bone Architecture

Cited 1 time
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Micro-CT (μCT) was used for nondestructive three-dimensional evaluation of cortical and cancellous bone volume and architecture as previously described (Hawse et al., 2014 (link)). In brief, femurs and LV5 were scanned in 70% ethanol at a voxel size of 12 × 12 × 12 μm using a Scanco μCT40 scanner (Scanco Medical AG, Brüttisellen, Switzerland). Total femur lengths (mm) were determined and cortical bone was evaluated in 20 slices (0.24 mm) in the femoral midshaft. Direct cortical measurements included cross-sectional tissue volume (TV, mm3), cortical volume (Ct.V, mm3), marrow volume (Ma.V, mm3), cortical thickness (Ct.Th, μm), and polar moment of inertia (IPolar, mm4). Total bone volume was also determined for LV5. This was followed by evaluation of cancellous bone at the femur metaphysis and in the vertebral body. Cancellous bone measurements included bone volume/tissue volume (BV/TV, %), trabecular number (Tb.N, mm−1), trabecular thickness (Tb.Th, μm), trabecular spacing (Tb.Sp, μm), and connectivity density (Conn.D, mm−3).
Mann S.N., Pitel K.S., Nelson-Holte M.H., Iwaniec U.T., Turner R.T., Sathiaseelan R., Kirkland J.L., Schneider A., Morris K.T., Malayannan S., Hawse J.R, & Stout M.B. (2020). 17α-Estradiol prevents ovariectomy-mediated obesity and bone loss. Experimental gerontology, 142, 111113.
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5

Microarchitecture Analysis of Bone Samples

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For μCT, spine and femur samples were placed into a 16mm tube filled with 70% ethanol and scanned, at a resolution of 16μm, using a ScanCo μct40 scanner (N=4–5, males). Trabecular and cortical bone analysis was performed using the ScanCo software, as previously described (13 (link)). Two-tailed, unpaired t-tests with unequal variances were used for statistical analysis.
Rajagopal A., Homan E.P., Joeng K., Suzuki M., Bertin T., Cela R., Munivez E., Dawson B., Jiang M.M., Gannon F., Crawford S, & Lee B.H. (2015). Restoration of the serum level of SERPINF1 does not correct the bone phenotype in Serpinf1−/− null mice. Molecular genetics and metabolism, 117(3), 378-382.
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6

Microstructural Analysis of Femoral Metaphysis

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The trabecular volume of the distal femoral metaphysis or proximal tibia was measured using a Scanco μCT40 scanner (Scanco Medical AG, Bassersdorf, Switzerland). A threshold of 250 was used for evaluation of all male scans and a threshold of 212 was used for evaluation of all female scans. 50 slices were analyzed, starting with the first slice in which condyles and primary spongiosa were no longer visible. Researchers were blinded in these experiments.
Zou W., Rohatgi N., Chen T.H., Schilling J., Abu-Amer Y, & Teitelbaum S.L. (2016). PPARγ Regulates Pharmacological but not Physiological nor Pathological Osteoclast Formation. Nature medicine, 22(11), 1203-1205.
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7

Quantitative Bone Microarchitecture Analysis

Cited 2 times
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Indices of trabecular and cortical bone volume and structure were measured in the spine (excised 5th lumbar spine body) and the femur, respectively, using a Scanco μCT-40 scanner (Scanco Medical, Bassersdorf, Switzerland). μCT scanning and analysis was performed as reported previously.(29 (link),42 (link)) Briefly, trabecular and cortical bone regions were evaluated using isotropic 12-mm voxels. For the vertebral trabecular region, we evaluated 250 transverse CT slices between the cranial and caudal end plates, excluding 100 μm near each end plate. For the femoral trabecular region, we analyzed 100 slices from the 50 slices under the distal growth plate. Femoral cortical bone was assessed using 50 continuous CT slides located at the femoral midshaft.
Li J.Y., Walker L.D., Tyagi A.M., Adams J., Weitzmann M.N, & Pacifici R. (2014). The Sclerostin-Independent Bone Anabolic Activity of Intermittent PTH Treatment Is Mediated by T-Cell–Produced Wnt10b. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 29(1), 43-54.
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8

3D Imaging of Zebrafish Heads

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Micro computed tomography (microCT) was used to generate three-dimensional imaging of ~8 month old adult zebrafish heads. Two male and two female adult zebrafish were scanned for each genotype. The fish were scanned using a Scanco μCT40 scanner (Scanco Medical AG, Basserdorf, Switzerland) at 45 kVp, 177 mA, and a voxel size of 12×12×12 mm (threshold = 140).
Garcia G.R., Bugel S.M., Truong L., Spagnoli S, & Tanguay R.L. (2018). AHR2 required for normal behavioral responses and proper development of the skeletal and reproductive systems in zebrafish. PLoS ONE, 13(3), e0193484.
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9

Measuring Cartilage Proteoglycan Content with EPIC μCT

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Equilibrium partitioning of an ionic contrast agent (EPIC) microcomputed tomography (μCT) was used to visualize the concentration and distribution of negative charge, as a measure for proteoglycan content in hydrogel constructs [20] (link), [21] (link). This technique uses a negatively charged contrast agent to indicate the amount and distribution of fixed negative charge in the hydrogel construct. Since cartilage ECM is negatively charged, gels with a higher amount of ECM, and therefore a higher negative charge, have a lower signal strength of contrast agent. Gels were incubated in a mixture of 40% Ioxaglate (Hexabrix, Aspen, Australia) in PBS at 37°C overnight with constant mixing, and imaged using a Scanco μCT 40 scanner (Scanco Medical, Brüttisellen, Switzerland).
Levett P.A., Hutmacher D.W., Malda J, & Klein T.J. (2014). Hyaluronic Acid Enhances the Mechanical Properties of Tissue-Engineered Cartilage Constructs. PLoS ONE, 9(12), e113216.
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10

Micro-CT Analysis of Mouse Skull Sutures

Cited 1 time
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Skulls from P0 mice were collected and stored in 70% ethanol prior to scanning. Skulls were scanned using a Scanco μCT40 scanner (Scanco Medical AG, Basserdorf, Switzerland) at 12 μm voxel size, 55 kVp X-ray voltage, 145 μA intensity, with a 200 ms integration time as previously described (29 (link)). Stacks of images were also individually analysed in MicroView (version 2.5.0; GE Healthcare Biosciences, London, Ontario, Canada) to extract sagittal sections of the coronal and lambdoid sutures in all genotypes of mice. Mouse work was performed in accordance with UK Home Office regulations under approved project licences and the Oregon State University Institutional Animal Care and Use Committee.
Goos J.A., Vogel W.K., Mlcochova H., Millard C.J., Esfandiari E., Selman W.H., Calpena E., Koelling N., Carpenter E.L., Swagemakers S.M., van der Spek P.J., Filtz T.M., Schwabe J.W., Iwaniec U.T., Mathijssen I.M., Leid M, & Twigg S.R. (2019). A de novo substitution in BCL11B leads to loss of interaction with transcriptional complexes and craniosynostosis. Human Molecular Genetics, 28(15), 2501-2513.
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