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Cd3 apc cy7 sp34 2

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CD3-APC/Cy7 (SP34-2) is a fluorochrome-conjugated monoclonal antibody used for the detection and identification of human T cells in flow cytometry applications.

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Lab products found in correlation

Cd28 ecd cd28 2, by Beckman Coulter (1 mentions) Golgi plug transport inhibitor, by BD (1 mentions) Rpa t8, by BioLegend (1 mentions) Bv421 conjugated streptavidin, by BioLegend (1 mentions) Ki67 pe b56, by BD (1 mentions) Live dead fixable blue viability dye, by Thermo Fisher Scientific (1 mentions) Transcription factor buffer set, by BD (1 mentions)

Close spelling variants of this product

CD3 APC-Cy7 (clone SP34-2; APC-Cy7 CD3 (Clone SP34–2) and V500 CD4 (clone L200) Anti-CD3 APC-Cy7 (clone SP34-2, Anti-CD3-APC-Cy7 (SP34–2), CD3-APC (SP34–2, CD3-APC-Cy7 SP34-2 APC-Cy7 CD3-APC

3 protocols using cd3 apc cy7 sp34 2

1

Multiparametric Flow Cytometry of Immune Cells

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Multiparametric flow cytometry was performed on PBMC, LNMC, and cells isolated from RBs using fluorescently labeled monoclonal antibodies cross-reactive in RMs. The following antibodies were used: CD3-APC/Cy7 (SP34-2), CD4-PE-CF594 (L200) CD8-BV711 (RPA-T8), CCR7-FITC (150503), CD45RA-PE/Cy7 (L48), CD95-PE/Cy5 (DX2), Ki67-AlexaFluor700 (B56), CD14-BV650 (M5E2), CD56-BV605 (NCAM16.2), CD62L-PE (SK11), HLA-DR-PerCPCy5.5 (G46.6), CCR5-APC (3A9) from BD Biosciences; CD28-ECD (CD28-2) from Beckman Coulter; CD16-BV421 (3G8), CD4-BV650 (OK-T4), PD-1-BV421 (EH12.2H7), from Biolegend; CD8-APC (DK25) from DAKO. All specimens were acquired on an LSR II (BD Biosciences) and analysis of the acquired data was performed using FlowJo software (Tree Star).
Cartwright E.K., Spicer L., Smith S.A., Lee D., Fast R., Paganini S., Lawson B.O., Nega M., Easley K., Schmitz J.E., Bosinger S.E., Paiardini M., Chahroudi A., Vanderford T.H., Estes J.D., Lifson J.D., Derdeyn C.A, & Silvestri G. (2016). CD8+ lymphocytes are required to maintain virus suppression in SIV-infected macaques treated with short-term antiretroviral therapy. Immunity, 45(3), 656-668.
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2

Quantifying Antigen-Specific Immune Responses

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Cell type-specific, antigen-specific immune responses were determined at indicated timepoints by flow cytometry using BAL cells and PBMC. Cells were stimulated overnight in the presence of Golgiplug transport inhibitor (BD; Biosciences).
The following mAb staining T cell panel was used for the dose-ranging study: Intracellular staining: CD3APC-Cy7 (SP34-2, BD; cat# 557757, dilution 1:320); CD69ECD (TP1.55.3, Beckman Coulter; cat# 6607110, dilution 1:50); IFN-γAPC (B27, BD; cat# 554702, dilution 1:320), TNF-αBV650 (Mab11, BD; cat# 563418, dilution 1:25), IL-2PE (MQ1-17H12, BD; cat# 544566, dilution 1:320); Surface staining: CD4PE-Cy5.5 (S3.5, Invitrogen; cat# MHCD0418, dilution 1:80), CD8BV570 (RPA-T8, Biolegend; cat# 301038, dilution 1:160), CD28Cy5-PE (CD28.2, BD; cat# 555730, dilution 1:10), CD45RAPE-Cy7, (L48, BD; cat# 337167, dilution 1:200).
Vierboom M.P., Chenine A.L., Darrah P.A., Vervenne R.A., Boot C., Hofman S.O., Sombroek C.C., Dijkman K., Khayum M.A., Stammes M.A., Haanstra K.G., Hoffmann C., Schmitt D., Silvestre N., White A.G., Borish H.J., Seder R.A., Ouaked N., Leung-Theung-Long S., Inchauspé G., Anantha R., Limbach M., Evans T.G., Casimiro D., Lempicki M., Laddy D.J., Bonavia A, & Verreck F.A. (2020). Evaluation of heterologous prime-boost vaccination strategies using chimpanzee adenovirus and modified vaccinia virus for TB subunit vaccination in rhesus macaques. NPJ Vaccines, 5, 39.
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3

Analyzing Env-specific GC B Cell Responses

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To assess Env-specific GC B cell responses, frozen LN cell suspensions were thawed and washed in R10, then stained with live/dead fixable blue viability dye (Invitrogen), tetramer Env probes in AF488 and BV421 for 30 min at 4°C. Cells were subsequently stained with anti-human CD20 BV570 (2H7, Biolegend), and CD3 APC-Cy7 (SP34–2, BD Biosciences) for an additional 20 min at 4°C. Cells were permeabilized using the transcription factor buffer set (BD Biosciences) and stained intracellularly for anti-human IgG BV786 (G18–145, BD Biosciences), BCL6 PE-Cy7 (K112–91, BD Biosciences), and Ki67 PE (B56, BD Biosciences). Tetramer Env probes were prepared by incubation of 4-fold molar excess of avi-tag biotinylated 1086 Env protein with either streptavidin-conjugated AF488 (Invitrogen) or streptavidin-conjugated BV421 (Biolegend).
Ols S., Yang L., Thompson E.A., Pushparaj P., Tran K., Liang F., Lin A., Eriksson B., Karlsson Hedestam G.B., Wyatt R.T, & Loré K. (2020). Route of Vaccine Administration Alters Antigen Trafficking but Not Innate or Adaptive Immunity. Cell reports, 30(12), 3964-3971.e7.
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