Microporous membrane transwell inserts (8 μm pore size, Costar, Washington, DC, USA) were used for the chemotaxis assay; 2 × 105 THP-1 cells in 200 μL RPMI were added to the upper chamber and the lower chamber containing the conditioned medium obtained from the THP-1 cell cultures after different cycles of intermittent hypoxia as attractants. The cells were allowed to migrate for 1 h and the nonmigratory cells were removed before the membrane was mounted. The number of migratory cells was revealed by Liu’s stain and counted under a microscope as described previously [25 (link)].
Microporous membrane transwell inserts
Manufactured by Corning
Sourced in United States, Switzerland
Microporous membrane transwell inserts are a type of laboratory equipment used to create a physical barrier between two compartments. The inserts consist of a porous membrane mounted on a permeable support structure, allowing for the passage of certain substances while restricting the movement of others. This core function enables researchers to study cell-cell interactions, cell migration, and transport processes in a controlled and reproducible environment.
Automatically generated - may contain errors
2 protocols using microporous membrane transwell inserts
1
Chemotaxis Assay for THP-1 Cells
2
HPLC Analysis of Doxorubicin and Daunorubicin
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Doxorubicin hydrochloride and daunorubicin hydrochloride (internal standard) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Calcein-AM was purchased from Molecular Probes (Eugene, OR, USA). Cell culture reagents were purchased from Lonza group Ltd. (Basel, Switzerland); microporous membrane transwell inserts were purchased from Corning Costar (Acton, MA, USA). Heptanesulfonic acid sodium salt (HPLC-grade) was purchased from TCI Co. Ltd. (Tokyo, Japan). Acetone, ZnSO4 and other reagents, including solvents, were of the highest analytical grade. Deionized water (18.2 MΩ, NANOpure diamond™, Brandstead water purification system, Fistreem International Co. Ltd. (Leicestershire, UK) was used throughout all HPLC analytical steps.
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