The cells were dissociated, the RNA was extracted, and their quality and quantity were assessed as described in the previous section. Libraries were sequenced using two sequencers, the Oxford Nanopore Technology ((ONT), San Francisco, CA, USA) and the PacBio (Pacific Biosciences, Menlo Park, CA, USA). Specific kits for library preparation were used whether the samples were sequenced on the PacBio or on the Oxford Nanopore Technology instruments. For the samples to be sequenced with the PacBio sequencer, the library preparation was performed using Iso-Seq Express Template Preparation for Sequel and Sequel II Systems (Pacific Biosciences, Menlo Park, CA, USA). For the samples sequenced with the Oxford Nanopore Technology system, the libraries were prepared using the Oxford Nanopore Technologies ligation-based kit (SQK-LSK109), Oxford Nanopore Technologies. The library size distributions were checked using Qubit 4 (ThermoFisher) and Tapestation (Agilent).
Iso seq express template preparation for sequel and sequel 2 systems
Manufactured by Pacific Biosciences
Sourced in United States
The Iso-Seq Express Template Preparation is a lab equipment product designed for use with Pacific Biosciences' Sequel and Sequel II Systems. It enables the preparation of template samples for long-read sequencing applications.
Automatically generated - may contain errors
2 protocols using iso seq express template preparation for sequel and sequel 2 systems
1
Nanopore and PacBio Sequencing of RNA
2
Nanopore and PacBio Sequencing of RNA
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The cells were dissociated, the RNA was extracted, and their quality and quantity were assessed as described in the previous section. Libraries were sequenced using two sequencers, the Oxford Nanopore Technology ((ONT), San Francisco, CA, USA) and the PacBio (Pacific Biosciences, Menlo Park, CA, USA). Specific kits for library preparation were used whether the samples were sequenced on the PacBio or on the Oxford Nanopore Technology instruments. For the samples to be sequenced with the PacBio sequencer, the library preparation was performed using Iso-Seq Express Template Preparation for Sequel and Sequel II Systems (Pacific Biosciences, Menlo Park, CA, USA). For the samples sequenced with the Oxford Nanopore Technology system, the libraries were prepared using the Oxford Nanopore Technologies ligation-based kit (SQK-LSK109), Oxford Nanopore Technologies. The library size distributions were checked using Qubit 4 (ThermoFisher) and Tapestation (Agilent).
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