Human adenocarcinoma alveolar basal epithelial (A549) cells (ATCC, VA, USA) and Madin-Darby Canine Kidney (MDCK) cells (ATCC, VA, USA) were maintained at 37°C at 5% CO2 in Dulbecco Modified Eagle Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (Gibco), 100U/mL penicillin-streptomycin (Gibco), 2mM GlutaMAX supplement (ThermoFisher). Expi293 cells (Gibco) were grown in Expi293 Expression Medium (ThermoFisher) at 37°C at 8% CO2 with regular agitation (150RPM). Trichoplusia ni High Five (ATCC) insect cells were maintained in HyClone SFX insect cell culture media (Cytiva/Fisher Scientific) at 28°C with regular agitation (70RPM). Spodoptera frugiperda Sf9 insect cells (ATCC) were maintained as adherent cultures in Roux flasks in full TNM-FH insect cell media (Sigma) supplemented with 10% FBS, 1% penicillin-streptomycin (Gibco) and 0.1% Pluronic F68 solution (Gibco) at 27°C.
Tnm fh insect cell media
Manufactured by Merck Group
TNM-FH is an insect cell media formulated for the cultivation of insect cells. It provides the necessary nutrients and supplements to support the growth and maintenance of insect cell cultures.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Heat inactivated fetal bovine serum, by Thermo Fisher Scientific (1 mentions)
A549 cell, by American Type Culture Collection (1 mentions)
Expi293 cell, by Thermo Fisher Scientific (1 mentions)
Pluronic f68 solution, by Thermo Fisher Scientific (1 mentions)
Expi293 expression medium, by Thermo Fisher Scientific (1 mentions)
Glutamax supplement, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
2 protocols using tnm fh insect cell media
1
Culturing Mammalian and Insect Cell Lines
2
Mammalian and Insect Cell Culture Protocols
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Madin-Darby Canine Kidney (MDCK) cells (ATCC, VA, USA) were maintained in Dulbecco Modified Eagle Medium (DMEM, Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco) and 100 U/mL penicillin-streptomycin (Gibco) at 37 °C in 5% CO2. Trichoplusia ni High Five (ATCC) insect cells were maintained in HyClone SFX insect cell culture media (Cytiva/Fisher Scientific) in shaker flasks shaking at 70 rpm at 28 °C.45 (link),46 (link)
Spodoptera frugiperda Sf9 insect cells (ATCC) were maintained as adherent cultures in Roux flasks in full TNM-FH insect cell media (Sigma) supplemented with 10% FBS, 1% penicillin-streptomycin and 0.1% Pluronic F68 solution at 27 °C.45 (link),46 (link)
Spodoptera frugiperda Sf9 insect cells (ATCC) were maintained as adherent cultures in Roux flasks in full TNM-FH insect cell media (Sigma) supplemented with 10% FBS, 1% penicillin-streptomycin and 0.1% Pluronic F68 solution at 27 °C.45 (link),46 (link)
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