Western blots were performed as described previously [43 ]. Rabbit primary antibodies (Cell Signaling Technology (CST), Danvers, MA) were used at the following dilutions: anti-p38α, -p38β, -p38γ, and -p38δ (1:1000), anti-ß-tubulin (1:2000), anti-p38 GAPDH (1:1000); anti-β-Actin (8H10D10), mouse mAb (1:2000). Anti-DLGH1 and anti-phosphor DLGH1 at serine 158 and 431, total DLGH1 (SAP97) are affinity-purified sheep polyclonal antibodies which were from University of Dundee, Scotland (1:1000). HRP-conjugated goat anti-rabbit (Cat#7074) and anti-mouse IgG, HRP-linked Antibody Cat#7076 (CST, 1:2000) were used as secondary antibodies.
Primary antibodies purchased from CST were used at the following dilutions: p38α (#9218, 1:1000), p38β (#2339, 1:1000), p38δ (#2308, 1:1000), p38γ (#2307, 1:1000), p-p38 Thr180Tyr182 (#4511, 1:1000), GAPDH (#2118, 1:5000), CBP (#7389, 1:1000), p300 (#86377, 1:1000), Ezh2 (#5246, 1:1000), TNFα (#3707, 1:1000), and Anti-SAPK3 (p38γ, ab205926) were purchased from abcam and used at 1:1000 dilution. p-p38 Tyr323 antibody (#12322-1) was purchased from Signalway Antibody and used at 1 μg/mL.
Primary antibodies purchased from CST were used at the following dilutions: p38α (#9218, 1:1000), p38β (#2339, 1:1000), p38δ (#2308, 1:1000), p38γ (#2307, 1:1000), p-p38 Thr180Tyr182 (#4511, 1:1000), GAPDH (#2118, 1:5000), CBP (#7389, 1:1000), p300 (#86377, 1:1000), Ezh2 (#5246, 1:1000), TNFα (#3707, 1:1000), and Anti-SAPK3 (p38γ, ab205926) were purchased from abcam and used at 1:1000 dilution. p-p38 Tyr323 antibody (#12322-1) was purchased from Signalway Antibody and used at 1 μg/mL.