Hearts were cut into pieces and fixed in 0.1 mol/L sodium phosphate buffer containing 2.5% glutaraldehyde for 3 h at 4°C, then osmicated in 1% osmium tetroxide for 1 h at 4°C. After dehydration of the tissues by using ethanol series, the samples were embedded in Epon 812 and sectioned using a Leica EM UC6 (Leica Coviema, Austria) ultra-microtome. Sections were viewed and photographed using a Hitachi 7650 TEM (Hitachi, Tokyo, Japan) at 80 kv.
7650 tem
Manufactured by Hitachi
Sourced in Japan
The Hitachi 7650 TEM is a transmission electron microscope designed for high-resolution imaging and analysis of materials at the nanoscale. It is capable of producing detailed, high-quality images of samples by transmitting a beam of electrons through a thin specimen.
Automatically generated - may contain errors
Lab products found in correlation
Osmium tetroxide, by Merck Group (2 mentions)
Em uc6, by Leica (1 mentions)
Uranyl acetate, by Polysciences (1 mentions)
Sodium cacodylate buffer, by Merck Group (1 mentions)
Lead citrate, by Polysciences (1 mentions)
Formaldehyde, by Biosharp (1 mentions)
Spurr s resin, by Polysciences (1 mentions)
Glutaraldehyde, by Solarbio (1 mentions)
Lead citrate, by Merck Group (1 mentions)
Uranyl acetate, by Merck Group (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
Jsm 5510, by JEOL (1 mentions)
Escalab 250, by Thermo Fisher Scientific (1 mentions)
12 protocols using 7650 tem
1
Cardiac Tissue Ultrastructural Analysis
2
Morphological Characterization of Grafted Microconjugates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To evaluate
the morphology of the grafted microconjugate variants,
the TEM was performed on a Hitachi-7650 TEM (Hitachi Co., Ltd., Japan)
at an acceleration voltage of 80 kV. The particle aqueous suspension
(0.05 wt %) was placed onto a Formvar-coated 200-mesh TEM grid (Canemco
Inc., Canada) and dried at room temperature before observation.
the morphology of the grafted microconjugate variants,
the TEM was performed on a Hitachi-7650 TEM (Hitachi Co., Ltd., Japan)
at an acceleration voltage of 80 kV. The particle aqueous suspension
(0.05 wt %) was placed onto a Formvar-coated 200-mesh TEM grid (Canemco
Inc., Canada) and dried at room temperature before observation.
3
Ultrastructural Imaging via TEM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were fixed with 2.5% glutaraldehyde at 4°C overnight. After removing the fixative, cells were then serially dehydrated in graded ethanol series, rinsed in propylene oxide, and incubated in propylene oxide and resin infiltration. Samples were embedded in Epon 812. The section was then visualized and photographed on Hitachi 7650 TEM (Hitachi, Japan).
4
Ultrastructural Changes in Aspergillus Biofilm
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TEM was performed to investigate the ultrastructural changes of A. fumigatus biofilm after exposure to CRA. In brief, 4 ml A. fumigatus suspension (4×105 conidia) was added to a 25 mm2 cell culture flask with a vented cap, followed by adhesion for 4 h. Subsequently, the supernatant containing non-adherent cells was removed, followed by incubation at 37°C for 24 h after adding fresh RPMI-1640 medium. For the CRA treatment group, CRA was added to fresh RPMI-1640 medium added after 4 h of adhesion. Biofilms were washed in PBS and fixed in a solution of 2.5% glutaraldehyde (Solarbio, Beijing, China) and 4% formaldehyde (Biosharp, Hefei, China) in 0.1 M sodium cacodylate buffer (pH 7.2, Sigma-Aldrich; Merck Millipore). Subsequently, the mixture was fixed for 2 h in the same buffer containing 1% osmium tetroxide (pH 7.2, Sigma-Aldrich, Merck Millipore), and then embedded in Spurr's resin (Polysciences, Warrington, USA). Ultrathin sections were stained with 4% uranyl acetate (Polysciences) and 2.6% lead citrate (Polysciences). Finally, the images were observed under a Hitachi 7650 TEM (Hitachi, Tokyo, Japan) at 80 kV.
5
Ultrastructural Analysis of Hippocampal Neurons
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After treatment and subsequent memory evaluation, the rats were randomly selected to be anesthetized with chloral hydrate (300 mg/kg, i.p.) and then perfused transcardially with normal saline followed by ~200 ml 2% paraformaldehyde and 2.5% glutaraldehyde (Tianjin Yongda Chemical Reagent Development Centre, Tianjin, China) to pre-fix the brain tissue. The brains were removed and fixed in 3% glutaraldehyde. The neuron ultrastructure in the hippocampal CA1 region was observed by transmission electron microscopy (TEM) according to a previously described method with certain modifications (23 (link)). In brief, the hippocampal CA1 area of the rats was rapidly excised, and then cut into pieces of 1 mm × 1 mm × 2 mm. The pieces were fixed in 3% glutaraldehyde for 2 h and post-fixed in 1% osmic acid (Beijing Zhongjingkeyi Technology Co., Ltd., Beijing, China) for 1.5 h. After that, the pieces were de-hydration-fixed by conventional ethanol and acetone treatments, embedded in ethoxyline resin 618 (Beijing Zhongjingkeyi Technology Co., Ltd.) and sliced using an ultra-thin slicer (UC7; Leica Microsystems, Wetzlar, Germany). Finally, the slices were double-stained with uranyl acetate (Beijing Zhongjingkeyi Technology Co., Ltd.) and lead citrate (Beijing Zhongjingkeyi Technology Co., Ltd.), and observed and filmed under a Hitachi-7650 TEM (Hitachi, Tokyo, Japan).
6
Ultrastructural Analysis of Murine Myocardium
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were evaluated cardiac function after LAD, hearts were cut into pieces and fixed in 0.1 mol/L sodium phosphate buffer containing 2.5% glutaraldehyde for 3 h at 4°C, then osmicated in 1% osmium tetroxide for 1 h at 4°C. After dehydration of the tissues by using ethanol series, the samples were embedded in Epon 812 and sectioned using a Leica EM UC6 (Leica Coviema, Austria) ultra-microtome. Sections were viewed and photographed using a Hitachi 7650 TEM (Hitachi, Tokyo, Japan) at 80 kv.
7
Ultrastructural Analysis of Lung Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The lower lobes of the right lung were cut into 1-2 mm cubes and fixed in 2.5% glutaraldehyde at 4°C immediately in the sealed container, and a syringe was used to empty the gas in the lung tissue. After sinking to the bottom, the lung specimens were then treated with osmium tetroxide and dehydrated in serial concentrations of ethanol. The tissues were treated with propylene oxide again, and then with a propylene oxide/epoxy resin mixture. Finally, they were embedded in labeled capsules with freshly prepared resin by Epon812. Sections were stained with uranyl acetate and lead citrate and observed under a Hitachi-7650 TEM (Hitachi, Tokyo, Japan) at 10000 to 20000x magnifications.
8
Amyloid-beta Aggregation in Betalain Pigments
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Aβ40 or Aβ42 was solubilized in DMSO or 10 mM NaOH to give a 1 mM solution, respectively. Aβ solutions were prepared with and without the addition of 50 µM red-beet betalain pigments and diluted to 20 µM with PBS. Aβ mixtures were incubated at 37 ºC was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint (which this version posted April 1, 2021. ; https://doi.org/10.1101/2020.12.23.424246 doi: bioRxiv preprint 13 for 5 days before TEM observations. Approximately 2 μL of sample solution was placed on a 150-mesh copper grid covered with formvar. After 5 min the sample was soaked away, then the grid was stained with 2.0% (w/v) uranyl acetate solution. TEM images of Aβ aggregates were obtained using a Hitachi 7650 TEM (Hitachi Co., Ltd., Tokyo, Japan) with an acceleration voltage of 80 kV.
The copyright holder for this preprint (which this version posted April 1, 2021. ; https://doi.org/10.1101/2020.12.23.424246 doi: bioRxiv preprint 13 for 5 days before TEM observations. Approximately 2 μL of sample solution was placed on a 150-mesh copper grid covered with formvar. After 5 min the sample was soaked away, then the grid was stained with 2.0% (w/v) uranyl acetate solution. TEM images of Aβ aggregates were obtained using a Hitachi 7650 TEM (Hitachi Co., Ltd., Tokyo, Japan) with an acceleration voltage of 80 kV.
9
TEM Analysis of Cochlear Explant Transfection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For TEM, the cochlear explants were cultured on polyethylene terephthalate membranes (EMD Millipore, Billerica, MA, USA). Samples were collected at various time-points (without transfection, and 0, 24 and 48 h post-transfection) and fixed in 2.5% glutaraldehyde (Sigma-Aldrich) in PBS at 4˚C for 6 h, followed by 1% osmium tetroxide (Sigma-Aldrich) at 4˚C for 1 hour, dehydration, infiltration and polymerization in araldite. Ultrathin sections (80-100 nm) were post-stained with uranyl acetate (Sigma-Aldrich) for 30 min in the dark and lead citrate (Sigma-Aldrich) for 6 min. The sections were then examined using a Hitachi-7650 TEM (Hitachi, Ltd, Tokyo, Japan). In all TEM experiments, 2 µg pDNA and 14 µg L-PEI was used.
10
Amyloid-beta Aggregation in Betalain Pigments
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Aβ40 or Aβ42 was solubilized in DMSO or 10 mM NaOH to give a 1 mM solution, respectively. Aβ solutions were prepared with and without the addition of 50 µM red-beet betalain pigments and diluted to 20 µM with PBS. Aβ mixtures were incubated at 37 ºC was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint (which this version posted April 1, 2021. ; https://doi.org/10.1101/2020.12.23.424246 doi: bioRxiv preprint 13 for 5 days before TEM observations. Approximately 2 μL of sample solution was placed on a 150-mesh copper grid covered with formvar. After 5 min the sample was soaked away, then the grid was stained with 2.0% (w/v) uranyl acetate solution. TEM images of Aβ aggregates were obtained using a Hitachi 7650 TEM (Hitachi Co., Ltd., Tokyo, Japan) with an acceleration voltage of 80 kV.
The copyright holder for this preprint (which this version posted April 1, 2021. ; https://doi.org/10.1101/2020.12.23.424246 doi: bioRxiv preprint 13 for 5 days before TEM observations. Approximately 2 μL of sample solution was placed on a 150-mesh copper grid covered with formvar. After 5 min the sample was soaked away, then the grid was stained with 2.0% (w/v) uranyl acetate solution. TEM images of Aβ aggregates were obtained using a Hitachi 7650 TEM (Hitachi Co., Ltd., Tokyo, Japan) with an acceleration voltage of 80 kV.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!