Jem 2100f transmission

Transmission electron microscopic images were measured through JEM-2100F transmission electron microscope (JEOL, Ltd., Japan) under 200 kV accelerating voltage. Energy dispersive X-ray spectroscopy (EDX) was also performed using a JEM-2100F EDX instrument. The morphology of the Ag-MSNs and the bacteria were inspected using a scanning electron microscope (FESEM, S4800, Hitachi Co. Ltd., Tokyo, Japan). The mesoporous properties were determined by the Brunauer-Emmett-Teller (BET) method through Micromeritics ASAP2010 surface area analyzer. The pore diameter was measured from the adsorption branch of the isotherm by the BJH method. X-ray powder diffraction (XRD) investigation was carried out on a Rigaku X-ray diffractometer using Cu Kα radiation. The size distribution and zeta potential were measured by Zetasizer (Nano ZS; Malvern Instruments, Malvern, UK). The silver content of the Ag-MSNs was measured via inductively coupled plasma mass spectrometer (ICP-MS) (Xseries II; Thermo Scientific, Waltham, MA, USA).

GBTPs suspension was diluted to 2 mM, and then 5 μL of the sample was added dropwise to the copper grid. The excess liquid was aspirated with filter paper (Situofan Biotechnology Co., Ltd., Hangzhou, China) after 30 s. Subsequently, 5 μL of the 1% uranyl acetate (Guangdong Fangxin Biotechnology Co., Ltd., Shantou, China) was placed on the grid and removed after 30 s. The sample was observed at 100 kV on a JEM-2100F transmission electron microscope (TEM, JEOL, Japan). A UV spectrophotometer (Genesys 10S, Thermo, USA) was used to measure the UV spectra of GA, BR, TA, and GBTPs solutions, with the scanning wavelength range of 200–600 nm.

JEM 2100F Transmission electron microscopy (JEOL company, Japan) were used to photograph the topography of dye molecules at 80 kV. In order to the observation of reactive Orange 13 dye aggregates, a drop of sample solution of Orange 13 dye was placed on 400-mesh formvar copper grids coated with carbon. After the water and solvent evaporate for about 5 minutes, and then observed the surface morphology.