Cycloheximide (chx)

1, 2-Dipalmitoyl-sn-glycero-3-phospho-rac-glycerol (DPPG-Na) and Soya Phosphatidyl Choline (LIPOID S100) were purchased from Lipoid GmbH (Friedenstrasse, Ludwigshafen, Germany); Cycloheximide was purchased from SERVA (Heidelberg, Baden-Württemberg, Germany). Topotecan Hydrochloride Capsules (XinZe^®) was obtained from Rui Nian Best (Nan Jing, Jiangsu, China); active ingredient: Topotecan-HCl). Medium-chain triglycerides (MCTs) were purchased from Hunan ER-KANG Pharmaceutical Co., Ltd, Shenzhen, China. The glipizide was used as internal standard (IS) (National Institute for the Control of Pharmaceutical and Biological, Beijing, China). High-performance liquid chromatography (HPLC) grade Methanol was bought from Fisher Scientific (Fair Lawn, NJ). Wahaha (Hangzhou, China) deionized water was used throughout the study.
(S)-10-[(dimethylamino)methyl]-4-ethyl-4,9-dihydroxy-(H-Pyrano[3’,4’:6,7]indolizino[1,2-b]quinoline-3,14[4H,12H]-dionemonohydrochloride (Topotecan Hydrochloride, TPT) was supplied by SanHuan Chemistry (WuHan, China) and other chemicals used without further purification were HPLC or analytical reagent grade purchased from local commercial suppliers.

Chemicals and reagents including rhamnolipids mixture R90 (AGAE Technologies) and 4-(Dimethylamino)pyridine (DMAP) were purchased from Sigma–Aldrich (Munich, Germany). Reagents used for coupling reactions including N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDCI) were purchased from ACROS Organics (Morris Plains, NJ, United States). Unless otherwise stated, all media components were purchased either from Oxoid (Cambridge, United Kingdom) or Becton Dickinson (Sparks, MD, United States).
Gentamycin sulfate and chloramphenicol were purchased from Sigma–Aldrich (Munich, Germany), tetracycline hydrochloride from USB Co. (Cleveland, OH, United States) and cycloheximide from SERVA (Heidelberg, Germany). The stock solutions of antibiotics were prepared as follows: gentamycin 30 g L^-1 in water, tetracycline 15 g L^-1 in ethanol, chloramphenicol 50 g L^-1 in ethanol, and cycloheximide 70 g L^-1 in water and were kept at -20°C.