Cells were pretreated with or without synthetic peptides (15 μg/ml) or A/C Heterodimerizer (Clontech), and then stained with 1 μg/ml CCL19-Ig (eBioscience, CA, USA) for 30 min, followed by PE-labeled anti-human IgG for 20 min on ice. CCL19-Ig binding levels were measured with a FACSVerse or FACSFortessa flow cytometer (BD Biosciences) and the data analyzed with FlowJo software (Tree Star Inc., OR, USA).
Ccl19 ig
Manufactured by Thermo Fisher Scientific
Sourced in United States
CCL19-Ig is a recombinant fusion protein consisting of the human chemokine CCL19 linked to the Fc region of human IgG1. It is a tool for research purposes.
Automatically generated - may contain errors
2 protocols using ccl19 ig
1
CCL19-Ig Binding Assay on Cells
2
CXCL12 and gp120 Mediated CCL19 Binding
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H9 cells were seeded on fibronectin-coated wells and treated with 0.1% BSA with PBS, CXCL12 (100 ng/ml), or recombinant gp120 (1 μg/ml) for 30 min. Binding of CCL19-Ig (1 μg/ml; eBioscience) to T cells was detected by staining with 5 μg/ml biotin-conjugated anti-human IgG (American Qualex) and 5 μg/ml Alexa Fluor 647-conjugated streptavidin (Invitrogen). The images were captured by a confocal microscope and analyzed with Duolink Image Tool software (Olink Bioscience, Uppsala, Sweden).
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