The largest database of trusted experimental protocols

Vt1000s

Manufactured by Vector Laboratories

The VT1000S is a compact and versatile laboratory equipment designed for general-purpose applications. It features a durable construction and provides consistent and reliable performance for a range of laboratory tasks.

Automatically generated - may contain errors

2 protocols using vt1000s

1

Brain Tissue Preparation for Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Virus injected mice were euthanized and perfused transcardially with PBS followed by 4% PFA (in PBS). After >24hr post-fix in 4% PFA, brains were sliced at 50μm thick using a vibrating blade microtome (Leica Biosystems VT1000S), mounted on glass slides with a DAPI mounting medium (Vectashield, H-1200), and imaged under a wide-field microscope with a 10x objective (VS120, Olympus). Images were acquired through OlyVIA 2.9 and processed via ImageJ 1.52i, which was also used for cell counting with local contrast enhancement (CLAHE) and particle analysis.
+ Open protocol
+ Expand
2

Brain Tissue Preparation for Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Virus injected mice were euthanized and perfused transcardially with PBS followed by 4% PFA (in PBS). After >24hr post-fix in 4% PFA, brains were sliced at 50μm thick using a vibrating blade microtome (Leica Biosystems VT1000S), mounted on glass slides with a DAPI mounting medium (Vectashield, H-1200), and imaged under a wide-field microscope with a 10x objective (VS120, Olympus). Images were acquired through OlyVIA 2.9 and processed via ImageJ 1.52i, which was also used for cell counting with local contrast enhancement (CLAHE) and particle analysis.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!