The Fourier transform infrared (FTIR) spectra of the samples in KBr pellets were measured on a Thermo Nicolet 670 FT-IR spectrophotometer (USA). In 32 scans, the spectrum of each sample from 4000 to 400 cm−1 was collected at a resolution of 4 cm−1.
Nicolet 670 ftir spectrophotometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Nicolet 670 FTIR spectrophotometer is a versatile analytical instrument used for the identification and quantification of chemical compounds. It operates by passing infrared radiation through a sample and measuring the absorption or transmission of the radiation by the sample. The resulting spectrum can be used to determine the molecular structure and composition of the sample.
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5 protocols using nicolet 670 ftir spectrophotometer
1
FTIR Spectroscopy of KBr Pellet Samples
2
Characterizing Plant Extract Composition
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FTIR analysis: A Nicolet 670 FTIR spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) was utilized to analyze the composition of extracts. Samples of Douglas fir, Scots pine and Korean fir cone extracts were placed at the output of infrared beams. The analysis of oscillating spectra allowed determination of the functional groups from polyphenols with which the radiation interacted.
UV–Vis analysis: The spectra of all cone extract samples at wavelengths of 190–1100 nm were recorded using a UV–Vis spectrophotometer (Evolution 220, Thermo Fisher Scientific, Waltham, MA, USA).
UV–Vis analysis: The spectra of all cone extract samples at wavelengths of 190–1100 nm were recorded using a UV–Vis spectrophotometer (Evolution 220, Thermo Fisher Scientific, Waltham, MA, USA).
3
Spectroscopic Characterization of Naringenin and Poly(naringenin)
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Fourier transform infrared spectroscopy (FTIR) (A Nicolet 670 FTIR spectrophotometer, Thermo Fisher Scientific, Waltham, MA, USA) was used to examine the specific chemical groups of naringenin and poly(naringenin) powders. The examination of oscillating spectra in the wavenumber range from 4000 to 400 cm−1 allows definition of the functional groups with which the radiation interacted. The ATR accessory equipped with a single reflection diamond ATR crystal was applied in all the measurements.
The UV-Vis spectra of naringenin and poly(naringenin) at wavelengths of 190–1100 nm were recorded utilizing a UV-Vis spectrophotometer (Evolution 220, Thermo Fisher Scientific). The measurement was performed for samples with a concentration of 1 mg/mL dissolved in ethanol (naringenin) or in distilled water (polymeric naringenin) [9 (link),20 (link)].
The UV-Vis spectra of naringenin and poly(naringenin) at wavelengths of 190–1100 nm were recorded utilizing a UV-Vis spectrophotometer (Evolution 220, Thermo Fisher Scientific). The measurement was performed for samples with a concentration of 1 mg/mL dissolved in ethanol (naringenin) or in distilled water (polymeric naringenin) [9 (link),20 (link)].
4
Structural Analysis of Poly(Flavonoid) by FTIR and UV-Vis
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FTIR analysis: A Nicolet 670 FTIR spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) was used to examine the structure of poly(flavonoid). Powders of monomeric naringenin and poly(naringenin) were placed at the output of infrared beams. The examination of oscillating spectra allows definition of the functional groups with which the radiation interacted. Based on the FTIR spectra, the degree of naringenin to poly(naringenin) conversion was determined.
UV-Vis analysis: The spectra of naringenin and poly(naringenin) samples at wavelengths of 190 to 1100 nm were recorded utilizing a UV-Vis spectrophotometer (Evolution 220, Thermo Fisher Scientific, Waltham, MA, USA).
UV-Vis analysis: The spectra of naringenin and poly(naringenin) samples at wavelengths of 190 to 1100 nm were recorded utilizing a UV-Vis spectrophotometer (Evolution 220, Thermo Fisher Scientific, Waltham, MA, USA).
5
Structural Analysis of Poly(catechin) via FTIR and UV-Vis
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A Nicolet 670 FTIR spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) was utilized to analyze the poly(catechin) structure. Samples of (+)-catechin and polymeric form of (+)-catechin were placed at the output of infrared beams. Oscillating spectra were obtained as the result of the test. The analysis of oscillating spectra allows determination of the functional groups with which the radiation interacted.
The spectra of (+)-catechin and poly(catechin) powders at wavelengths of 190–1100nm were recorded using a UV-Vis spectrophotometer (Evolution 220, Thermo Fisher Scientific, Waltham, MA, USA).
The spectra of (+)-catechin and poly(catechin) powders at wavelengths of 190–1100nm were recorded using a UV-Vis spectrophotometer (Evolution 220, Thermo Fisher Scientific, Waltham, MA, USA).
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