Nifedipine nif

Manufactured by Merck Group

Sourced in Japan

Nifedipine (Nif) is a calcium channel blocker used as a pharmaceutical active ingredient in various medical products. It functions by inhibiting the movement of calcium ions across cell membranes, which can help regulate blood pressure and heart rate. Nifedipine is a widely used medication, but its specific applications and intended uses should be determined by healthcare professionals.

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Nifedipine (381 citations)

5 protocols using nifedipine nif

Fluorescent Calcium Imaging Assay

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Nifedipine (NIF) and caffeine (caf) were obtained from Sigma-Aldrich. The RU-28318, thapsigargin (Tg) and the KB-R7943 were obtained from TOCRIS Bioscience (Bristol, UK). Fluo-4/AM and Fura-2/AM were obtained from Thermo Fisher Scientific (Waltham, MA, USA).

Bartoli F., Moradi Bachiller S., Antigny F., Bedouet K., Gerbaud P., Sabourin J, & Benitah J.P. (2019). Specific Upregulation of TRPC1 and TRPC5 Channels by Mineralocorticoid Pathway in Adult Rat Ventricular Cardiomyocytes. Cells, 9(1), 47.

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Pharmacological Evaluation of Novel Compounds

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The following drugs were used: PregS (Sigma‐Aldrich), nifedipine (Nif, Sigma‐Aldrich), Dic (Wako), aspirin (Asp, Wako), ibuprofen (Ibu, Wako), loxoprofen (Lox, Wako), Ind (Wako), etodolac (Etd, Wako), piloxicam (Pil, Wako), niflumic acid (Nfm, Sigma‐Aldrich), aceclofenac (Afc, Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), GSK1016790A (GSK; Sigma‐Aldrich), hydrogen peroxide (H₂O₂, Sigma‐Aldrich), and menthol (Men, Wako). Each drug was dissolved in the vehicle recommended by the manufacturer.

Suzuki H., Sasaki E., Nakagawa A., Muraki Y., Hatano N, & Muraki K. (2016). Diclofenac, a nonsteroidal anti‐inflammatory drug, is an antagonist of human TRPM3 isoforms. Pharmacology Research & Perspectives, 4(3), e00232.

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Blocking Ca2+ Channels and Pumps

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For blocking of different Ca²⁺ channels and pumps: 1 µM CdCl₂ for L, P/Q, N, R and T type, 50 nM SNX482 for R type, 10 µM nifedipine (Nif) for L type (all from Sigma) and 100 µM NiCl₂ for R, T type (from Roth) plasma membrane Ca²⁺ channel; 1 µM thapsigargin (Thap) for Serca 1; 2; 3 (Sigma) were used. To ensure full effect of the Ca²⁺ channel modifiers, DRG with these modifiers were incubated for 60 min.

Treinys R., Kaselis A., Jover E., Bagnard D, & Šatkauskas S. (2014). R-Type Calcium Channels Are Crucial for Semaphorin 3A–Induced DRG Axon Growth Cone Collapse. PLoS ONE, 9(7), e102357.

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Bdph Synthesis and Characterization

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Bdph was synthesized and yielded a light yellow oily substance according to the previously described method [14 ]. Its purity (99.8%) was analyzed by using high performance liquid chromatography and the structure is shown in Figure 1 [15 ]. 4-Aminopiridine (4-AP), atropine sulfate, α-chymotrypsin, diltiazem, glibenclamide (GBC), histamine diphosphate, indomethacin, nicardipine, nifedipine (Nif), pyrilamine maleate, tetraethylammonium bromide (TEA), and verapamil (Vrp) were purchased from Sigma-Aldrich, St. Louis, MO. U.S.A. Papaverine was purchased from Narcotics Bureau, Taipei, Taiwan. Cromakalim, methysergide, and FPL 557121 were gifts from SmithKline Beecham Pharmaceutical, U.K., Sandoz, Swiss, and Fisons, U.K., respectively.
Male Hartley guinea-pigs (250~400 g) were obtained from the Animal Center of the National Science Council (Taipei, Taiwan). The animals were housed in ordinary cages at 22 ± 1°C with a humidity of 50%~60% under a constant 12/12-h light/dark cycle and provided with food and water ad libitum. Under a protocol approved by the Animal Care and Use Committee of Taipei Medical University, the following in vitro experiments were performed.

Hsu H.T., Yang Y.L., Chen W.C., Chen C.M, & Ko W.C. (2014). Butylidenephthalide Blocks Potassium Channels and Enhances Basal Tension in Isolated Guinea-Pig Trachea. BioMed Research International, 2014, 875230.

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Investigating Ion Channel Modulators

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The following reagents were used: allyl-isothiocyanate, (AITC, Sigma), 4-(3-chloro-2-pyridinyl)-N-[4-(1,1-dimethylethyl)phenyl]-1-piperazinecarboxamide (BCTC, Sigma), bradykinin (BK), capsaicin (Caps, Alomone Labs), ω-conotoxin GIVA (Ascent scientific), forskolin (Fors, Abcam), HC-030031 (Sigma), Histamine (His, Sigma), nifedipine (Nif, Sigma), oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (OxPAPC, Hycultech), PGPC (Avanti Polar Lipids), prostaglandin E2 (PGE₂, Sigma), tetrodotoxin (TTX, Ascent scientific). Dimethyl sulfoxide, chloroform or aqueous physiological solutions served as solvents.

Martin C., Stoffer C., Mohammadi M., Hugo J., Leipold E., Oehler B., Rittner H.L, & Blum R. (2018). NaV1.9 Potentiates Oxidized Phospholipid-Induced TRP Responses Only under Inflammatory Conditions. Frontiers in Molecular Neuroscience, 11, 7.

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