Il 18 elisa kit

Manufactured by MultiSciences Biotech

Sourced in China

The IL-18 ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of interleukin-18 (IL-18) levels in various sample types. It utilizes two specific antibodies, one for capture and one for detection, to detect the presence and quantity of IL-18 in the sample.

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Spelling variants of this product

IL-18 (6 citations) Human IL-18 ELISA kit (4 citations) ELISAs (2 citations) Mouse IL-18 ELISA kit (2 citations)

7 protocols using il 18 elisa kit

Quantification of Cytokine Secretion

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Supernatants from six-well plates transfected with siRNAs or plasmids and stimulated with TNF-α were harvested. Cytokines were quantified using mouse IL-1β and interleukin-18 (IL-18) ELISA kits (Multisciences, Hangzhou, China) following the manufacturer's instructions.

Tang H., Gong X., Dai J., Gu J., Dong Z., Xu Y., Hu Z., Zhao C., Deng J, & Dong S. (2023). The IRF1/GBP5 axis promotes osteoarthritis progression by activating chondrocyte pyroptosis. Journal of Orthopaedic Translation, 44, 47-59.

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Peptide MSI-1 Characterization and Inhibition

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Peptide MSI‐1 (purity > 98%) were synthesized by GL Biochem Co., Ltd. Polymyxin B sulphate (CAS1405‐20‐5; purity 99%) and Cytoplasmic and Mitochondrial Protein Extraction Kit were purchased from Sangon Biotech Co., Ltd. Isopropyl β‐D‐Thiogalactoside (IPTG) and 1,6‐diphenyl‐1,3,5‐hexatirene (DPH) were purchased from Aladdin Biochem Technology Co., Ltd. Specific TLR4 inhibitor Resatorvid (TAK₂₄₂; HY‐11109), caspase‐11 inhibitor wedelolactone (Wed; HY‐N0551), JC‐1 Assay Kit and Cytotoxicity LDH Assay Kit were purchased from MCE Co., Ltd. 1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethylindocarbocyanine perchlorate (Dil), DAPI, actin‐tracker green, LysoTracker red and 2, 7‐dichlorofluorescein diacetate (DCFH‐DA) were purchased from Beyotime. TNF‐α, IL‐6, IL‐1β and IL‐18 ELISA Kits were purchased from MultiSciences Biotech Co., Ltd.

Ye X., Wang J., Xu P., Yang X., Shi Q., Liu G., Bai Z., Zhou C, & Ma L. (2023). Peptide MSI‐1 inhibited MCR‐1 and regulated outer membrane vesicles to combat immune evasion of Escherichia coli. Microbial Biotechnology, 16(9), 1755-1773.

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Quantifying IL-18 and IL-1β in Cell Supernatants and Sera

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The supernatant from treated MC38 cells and CT26 cells, and the serum from the collected mice blood were measured with IL-18 ELISA kits and IL-1β ELISA kits (Multisciences, CHN). Operation was performed according to the manufacturer's instructions. Then, the absorbance was gauged at 450 nm.

Hu D., Cui L., Zhang S., He S., Zhuo Y., Li D., Zhang L., Wang Y., Yang L, & Wang X. (2024). Antitumor effect of tubeimoside-I on murine colorectal cancers through PKM2-dependent pyroptosis and immunomodulation. Naunyn-Schmiedeberg's archives of pharmacology, 397(6).

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Inflammatory Cytokine Quantification

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Serum IL-1β and IL-18 concentrations were measured using an ELISA kit according to manufacturer's instructions. IL-1β ELISA kit was from RayBiotech, Inc., Norcross, GA, USA, IL-18 ELISA kit (Cat# EK218) was from MultiSciences Biotech Co. (Hangzhou, China)

Yan B., Zhang Y., Liang C., Liu B., Ding F., Wang Y., Zhu B., Zhao R., Yu X.Y, & Li Y. (2020). Stem cell-derived exosomes prevent pyroptosis and repair ischemic muscle injury through a novel exosome/circHIPK3/ FOXO3a pathway. Theranostics, 10(15), 6728-6742.

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Quantification of IL-1β and IL-18 in Rat Hippocampus

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Rat hippocampal tissues were homogenized on ice in saline to extract protein and protein concentration was quantified using a BCA protein quantification kit (Beijing Solarbio Science and Technology Co., Ltd.). The amount of IL-1β and IL-18 was determined using a commercial IL-1β ELISA kit [cat. no. 70-EK301B/3-96; MultiSciences (Lianke) Biotech Co. Ltd.] and IL-18 ELISA kit (cat. no. SEA064Ra; Wuhan USCN Business Co., Ltd.), according to the manufacturers' instructions.

Zhang X., Fan X., Li F., Qiu J, & Zhang Y. (2020). Effects of PYRIN-containing Apaf1-like protein 1 on isoflurane-induced postoperative cognitive dysfunction in aged rats. Molecular Medicine Reports, 22(2), 1391-1399.

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Inflammatory Factors Quantification in LG

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The levels of IL-1β and IL-18 inflammatory factors in LG lysates were detected using mouse IL-1β ELISA kit and IL-18 ELISA kit (Multi Sciences Biotech Co. Ltd., Hangzhou, China). ELISA was performed according to the manufacturer's instructions. Absorbance was read at 450 nm with a reference wavelength of 570 nm by a full-wavelength microplate reader (SpectraMax190).

Chen Y., Pu J., Li X., Lian L., Ge C., Liu Z., Wang W., Hou L., Chen W, & Li J. (2023). Aim2 Deficiency Ameliorates Lacrimal Gland Destruction and Corneal Epithelium Defects in an Experimental Dry Eye Model. Investigative Ophthalmology & Visual Science, 64(3), 26.

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Serum Biomarker Quantification Protocol

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Serum and cell supernatants were collected and CK-MB, LDH, IL-1βand IL-18 levels in serum and cell supernatants according to instructions. The CK-MB and LDH kits were purchased from Nanjing Jiancheng Bioengineering Institute, China. The IL-1βELISA kit and the IL-18 ELISA kit were purchased from MULTISCIENCES (LIANKE) BIOTECH, CO., LTD

Li w., Shen X., Feng S., Liu Y., Hu R., Zhou G., Sang M., Sun X., Jiao R., & Liu F. (2022). BRD4 inhibition by JQ1 protects against LPS-Induced Cardiac Dysfunction by inhibiting SIRT1-dependent activation of NLRP3 inflammasomes.

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