Applied cell extracellular matrix (Cat#G422), Prigrow I medium (Cat#TM001), Prigrow III medium (Cat#TM003), Prigrow IV medium (Cat#TM004), and PriCoat T25 flasks (Cat#G299) were purchased from Applied Biological Materials Inc (Richmond, BC, Canada). X-vivo 15 serum-free medium (Cat#04-418Q) was purchased from Lonza Inc. GM-CSF and IL-4 were purchased from Peprotech (Rocky Hill, NJ). FBS and Superscript IV First-Strand Synthesis Kit were purchased from Invitrogen (Carlsbad, CA). Trizol reagent was purchased from Ambion. TaqMan Gene Expression Master Mix was purchased from Applied Biosystems. RNeasy Mini Kit, RNase-Free DNase Set, mouse Dopamine/Serotonin (Cat#PAMM-158Z), mouse GABA/Glutamate (Cat#PAMM-152Z) RT2 Profiler PCR Arrays and SYBR green reaction master mix were purchased from Qiagen. Ketamine (100 mg/ml) was obtained from the Wexner Medical Center Pharmacy at The Ohio State University and xylazine hydrochloride was purchased from Sigma. The Human L-1000 Antibody Arrays were purchased from RayBiotech.
Human l 1000 antibody arrays
Manufactured by RayBiotech
Sourced in United States
The Human L-1000 Antibody Arrays are a multiplex assay platform designed to detect and quantify the levels of 1,000 different human proteins simultaneously. The arrays utilize antibodies specific to each target protein, allowing for the simultaneous measurement of a wide range of analytes in a single sample. This technology provides a comprehensive and efficient method for analyzing complex protein expression patterns.
Automatically generated - may contain errors
Lab products found in correlation
Rneasy mini kit, by Qiagen (1 mentions)
Taqman gene expression master mix, by Thermo Fisher Scientific (1 mentions)
X vivo 15 serum free medium, by Lonza (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions)
Gm csf, by Thermo Fisher Scientific (1 mentions)
Prigrow 1 medium, by Applied Biological Materials (1 mentions)
Prigrow 4 medium, by Applied Biological Materials (1 mentions)
Prigrow 3 medium, by Applied Biological Materials (1 mentions)
Xylazine hydrochloride, by Merck Group (1 mentions)
Rt2 profiler pcr array, by Qiagen (1 mentions)
Rnase free dnase set, by Qiagen (1 mentions)
Pricoat t25 flasks, by Applied Biological Materials (1 mentions)
Applied cell extracellular matrix, by Applied Biological Materials (1 mentions)
2 protocols using human l 1000 antibody arrays
1
Optimizing Neuronal Cell Culture Conditions
2
GAB2 Expression Impacts on Cellular Secretome
Check if the same lab product or an alternative is used in the 5 most similar protocols
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In total, 1 × 106 of FTSECs expressing GAB2 or a control vector were plated into 10-cm culture dishes for 24 h. The media were replaced with serum-deprived media containing 0.1% fetal bovine serum, and the cells were cultured for 48 h. The culture supernatants were collected, centrifuged at 1000 × g, and dialyzed with 2 l of 1 × phosphate-buffered saline (pH 8) twice for overnight at 4oC. Samples were labeled with biotin and incubated with Human L1000 Antibody Arrays (#AAH-BLM-1000, RayBiotech, Norcross, GA, USA). Representative images from two independent experiments were shown.
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