Axio imager lsm 800

Manufactured by Zeiss

Sourced in Germany

The Axio-Imager LSM-800 is a laser scanning microscope designed for high-resolution imaging. It features an advanced optical system and a range of specialized imaging modes to capture detailed, high-quality images of samples.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Beyotime (3 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (3 mentions) Antifade mounting medium, by Beyotime (2 mentions) Pkh67, by Thermo Fisher Scientific (2 mentions) Pkh67, by Merck Group (2 mentions) Fitc conjugated secondary antibody, by Cell Signaling Technology (1 mentions) Alexa fluor 594 conjugated donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions) Image pro plus version 6, by Media Cybernetics (1 mentions) Alexa fluor 488 donkey anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions) Ribo fluorescent in situ hybridization kit, by RiboBio (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Paraformaldehyde (pfa), by Sangon (1 mentions) Triton x100, by Leagene (1 mentions) Cell climbing slices, by Solarbio (1 mentions) Ya0350, by Solarbio (1 mentions) Anti rabbit igg whole molecule fitc antibody, by Merck Group (1 mentions) Laser scanning microscope, by Zeiss (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Cell Signaling Technology (1 mentions) Rhodamine conjugated secondary antibody, by Cell Signaling Technology (1 mentions) Ab243641, by Abcam (1 mentions)

Close spelling variants of this product

LSM 800 (2673 citations) Axio Imager (671 citations) Axio-Imager_LSM-800 confocal microscope (2 citations)

21 protocols using axio imager lsm 800

Transient Expression of AcBBX5 in Tobacco

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The coding sequence of AcBBX5 was introduced into the pCAMBIA2300-GFP vector digested with Kpn I and Xba I restriction enzymes to generate the construct 35S::AcBBX5-GFP. After sequencing correctly, it was transformed into Agrobacterium tumefaciens strain GV3101. The epidermal cell transformation of tobacco leaves was injected with the A. tumefaciens carrying out with the recombinant vector. After incubation in the dark for 12 h at 25 °C, the tobaccos were transferred to normal growth for 24–36 h. The fluorescence signal was observed by a confocal scanning microscope Ax-io-Imager_LSM-800 (Zeiss, Germany) under excitation of 488 nm.
A yeast assay system was used to examine the transcriptional activity of AcBBX5. Nde I and Sal I were selected as restriction sites, and the coding sequences of AcBBX5 were inserted into the bait vector pGBKT7 by homologous recombination. Following the manufacturer’s protocol, the recombinant vectors were transferred into AH109 strain yeast and cultured on SD/-Trp medium at 30 °C for 2–3 days. Single colonies were selected for amplification culture and then transferred to SD/-Trp and SD/-Trp/-His/-Ade solid media for further culture. Three days later, X-α -Gal was added to observe whether the colony was blue.

Ouyang Y., Zhang X., Wei Y., He Y., Zhang X., Li Z., Wang C, & Zhang H. (2022). AcBBX5, a B-box transcription factor from pineapple, regulates flowering time and floral organ development in plants. Frontiers in Plant Science, 13, 1060276.

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Localization of LINC01134 and IGF2BP2

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The two assays were utilized to localize LINC01134 and IGF2BP2 in SNU-182 and SK-HEP-1 cells. At first, 4% PFA was utilized for 15 min fixation at 37°C. After being permeabilized with 0.5% Triton X-100 (R00285, Leagene, Beijing, China), HCC cells were co-cultured with LINC01134 FISH probe in hybridization buffer and then stained with DAPI.
For the IF assay, IGF2BP2 primary antibody was added to incubate with cells at 4°C for a whole night. Then, FITC-conjugated secondary antibody (7076, Cell Signaling Technology, Boston, MA, United States) was added. With the help of a confocal laser microscope (Axio-Imager_LSM-800, Zeiss, Oberkochen, Germany), images were gained. The experiment was independently conducted in triplicate.

Wang Z., Wang X., Rong Z., Dai L., Qin C., Wang S, & Geng W. (2022). LncRNA LINC01134 Contributes to Radioresistance in Hepatocellular Carcinoma by Regulating DNA Damage Response via MAPK Signaling Pathway. Frontiers in Pharmacology, 12, 791889.

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Immunofluorescence Staining of PRRSV-N Protein

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Cells seeded in 24-well plates were fixed with 4% paraformaldehyde (precooled at 4 °C) for 10 min and then immediately permeabilized with methanol (precooled at −30 °C) for 15 min prior to the addition of blocking reagent (5% BSA). Next, cells were incubated with the primary antibody against PRRSV-N protein for 1 h and washed three times with PBS, followed by incubation with Alexa Fluor 594-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch, PA, USA). Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; Beyotime, Shanghai, China) for 45 min. Fluorescent images were acquired with Axio-Imager_LSM-800 (Zeiss, Germany).

Long S., Zhou Y., Bai D., Hao W., Zheng B., Xiao S, & Fang L. (2019). Fatty Acids Regulate Porcine Reproductive and Respiratory Syndrome Virus Infection via the AMPK-ACC1 Signaling Pathway. Viruses, 11(12), 1145.

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Retinal Tissue Fixation and Analysis

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Under anesthesia, 0.1 M PBS (pH 7.4) containing ice-cold 4% PFA was injected into the left cardiac ventricle of the rats for fixation before the eyeballs of the rats were removed and embedded in 4% PFA at 4°C for 1 day. After fixation, the eyeballs were dehydrated, embedded in paraffin and cut into 5-µm sections. The central part of the eyeballs, through the optic nerve, were selected for immunohistochemical analysis. Each section was heated with 10 mM sodium citrate buffer (pH 6.0) at the secondary boiling point (100°C) for 10 min and then cooled for 30 min for antigen retrieval. Blocking, primary and secondary antibody labelling, and all washing steps were performed as mentioned above. Staining was observed using a confocal microscopy at a magnification of x20 (Axio-Imager_LSM-800, ZEISS, Germany). H&E staining was observed using an inverted fluorescence microscope at a magnification of x20 (Nikon Corporation). The thicknesses of the inner retina [RGC + inner plexiform layer (IPL) + inner nuclear layer (INL)] were measured in the H&E sections using the Image-pro Plus version 6.0 (Media Cybernetics) at every quarter point of each retinal cross-section, which were then averaged. Each group contained four eyeballs to be measured. A total of 10 images were obtained from each retinal section of every eyeball for measuring the average.

Pang Y., Qin M., Hu P., Ji K., Xiao R., Sun N., Pan X, & Zhang X. (2020). Resveratrol protects retinal ganglion cells against ischemia induced damage by increasing Opa1 expression. International Journal of Molecular Medicine, 46(5), 1707-1720.

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Apoptosis Analysis in Retinal Cells

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Apoptosis analysis in retinal cells was performed as described previously (25 (link),27 (link)). The preparation of sections (5 µm) and the treatment of R28 cells were described above, following which a TUNEL assay was performed using In Situ Cell Death Detection kit, Fluorescein (Roche Applied Science) to detect apoptotic cells in accordance with manufacturer's protocol. Tissue sections were assessed using a confocal microscope at a magnification of x20 (Axio-Imager_ LSM-800, ZEISS, Germany). Digital images were obtained (SPOT; Diagnostic Instruments, Inc.) and Photoshop versions 5.5 and 7.0 (Adobe Systems, Inc.) was used to compile images.

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VE-cadherin expression in HUVEC

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HUVECs was on the coverslips and transfected with the expression plasmids of HCG18 or SOX7 or miR-21-5p mimics, and then treated with PM2.5. Cells were fixed in 4% polyoxymethylene and permeated in 1% Triton X-100. Then, cells were blocked using 1% BSA and incubated with rabbit anti-VE-cadherin antibody and Alexa Fluor 488 donkey anti-rabbit IgG (H+L) (Life Technology, USA). Following it, cells were stained with DAPI and photographed using a Laser scanning confocal microscope (Axio-Imager_LSM-800, ZEISS).

Wang S., Lin Y., Zhong Y., Zhao M., Yao W., Ren X., Wang Q., Guo X., Zhang Q.Q, & Dai J. (2020). The long noncoding RNA HCG18 participates in PM2.5-mediated vascular endothelial barrier dysfunction. Aging (Albany NY), 12(23), 23960-23973.

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FISH Analysis of MBNL1-AS1 Expression

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Three experiments were performed independently. Ribo™ Fluorescent In Situ Hybridization Kit procured from RiboBio (Guangzhou, China) was applied as per the user guide. The MBNL1-AS1 probe was purchased by RiboBio. DAPI (D9542, Sigma-Aldrich, St. Louis, MO, USA) was applied for nuclear staining. Images of both H9c2 and H9c2/Hypoxia cells were captured by confocal microscope (Axio-Imager_LSM-800, Zeiss, Oberkochen, Germany).

Li Y., Zong M., Guan X., Wu X., Ma G., Wei Y, & Li Z. (2023). MBNL1-AS1 Promotes Hypoxia-Induced Myocardial Infarction via the miR-132-3p/RAB14/CAMTA1 Axis. Oxidative Medicine and Cellular Longevity, 2023, 3308725.

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Quantifying FUT8-AS1 Localization in OSCC

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After 15-minute fixation with paraformaldehyde (4%; Sangon Biotech) at 37°C, 8000 to 10,000 OSCC cells at 20% to 30% confluence were permeabilized with 0.5% Triton X-100 (Leagene, Beijing, China) and then hybridized with fluorescent in situ hybridization probe targeting FUT8-AS1 (Invitrogen) in buffer, followed by counterstaining with DAPI solution or Hoechst. Confocal laser microscope (Axio-Imager_LSM-800; Zeiss, Oberkochen, Germany) was used for observation. Fluorescent in situ hybridization experiments were performed three times.

Geng Z., Huang Y., Wu S., Zhu D, & Li W. (2023). FUT8-AS1/miR-944/Fused in Sarcoma/Transcription Factor 4 Feedback Loop Participates in the Development of Oral Squamous Cell Carcinoma through Activation of Wnt/β-Catenin Signaling Pathway. The American journal of pathology, 193(2).

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Immunofluorescence Staining of PEDV in IPEC-J2 Cells

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Cell climbing slices (Solarbio, Beijing, China; YA0350) were placed at the bottom of the wells of a 24-well plate before seeding with IPEC-J2 cells. PEDV LJX infection, control, and PEDV CV777 infection groups were set up with three repeat wells for each group. The slices were washed with PBS thrice for 5 min each, followed by fixing in 4% paraformaldehyde for 30 min. The slices were again washed with PBS thrice for 5 min each, and then they were blocked with 5% bovine serum albumin (BSA) for 60 min. They were then washed with PBS three times for 3 min each and incubated with the primary antibodies in a wet dish at 4 °C overnight. After washing with PBS thrice for 3 min each, the cells were incubated with anti-rabbit IgG cyanine 3 (Cy3) Fragment antibody (Cell Signaling Technology, Danvers, MA, USA) for 30 min, and then washed three times. Staining was performed using 4′,6-diamidino-2-phenylindole (DAPI) (Beyotime, Jiangsu, China) for 5 min, followed by three PBS washes for 5 min each. Lastly, 3–5 drops of antifade mounting medium (Beyotime) were added, and the samples were visualized using laser confocal microscopy (Axio-Imager LSM-800, Carl Zeiss AG, Oberkochen, Germany).

Niu Z., Zhang S., Xu S., Wang J., Wang S., Hu X., Zhang L., Ren L., Zhang J., Liu X., Zhou Y., Yang L, & Song Z. (2023). Porcine Epidemic Diarrhea Virus Replication in Human Intestinal Cells Reveals Potential Susceptibility to Cross-Species Infection. Viruses, 15(4), 956.

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Immunofluorescence Analysis of IPEC-J2 Cells

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Cell climbing slices (Solarbio, YA0350, CN) were placed at the bottom of 24-well and before seeding IPEC-J2 cells. TGEV infected group, control group, siRNA groups and Phlorizin group were set up, 3 repeat well for each group. The slices were washed by PBS thrice for 5 min each time, followed by fixing in 4 % paraformaldehyde for 30 min. The slices were again washed with PBS thrice for 5 min each time, they were blocked with 5 % BSA for 60 min, followed by PBS washes thrice for 3 min each and incubated with the primary antibodies in a wet dish at 4 °C overnight. Washing with PBS thrice for 3 min each time. Then incubation with Anti-rabbit IgG (H + L) F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) antibody (Cell Signaling Technology) and Anti-Rabbit IgG (whole molecule)–FITC antibody (Sigma-Aldrich) for 30 min, washing three times. Staining was performed using DAPI (Beyotime, CN) for 5 min, followed by PBS washed thrice for 5 min each, add 3–5 drops of antifade mounting medium (Beyotime, CN). The samples were visualized by laser confocal microscopy (Axio-Imager LSM-800, Carl Zeiss AG).

Yang Y., Yu Q., Song H., Ran L., Wang K., Xie L., Huang S., Niu Z., Zhang Y., Kan Z., Yan T, & Song Z. (2020). Decreased NHE3 activity and trafficking in TGEV-infected IPEC-J2 cells via the SGLT1-mediated P38 MAPK/AKt2 pathway. Virus Research, 280, 197901.

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