Anti h3k9me1

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-H3K9me1 is a lab equipment product that detects and binds to monomethylated histone H3 at lysine 9 (H3K9me1). It is used for the analysis and detection of this specific histone modification.

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Lab products found in correlation

4 protocols using anti h3k9me1

Immunofluorescence Analysis of Cellular Markers

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HRP-conjugated anti-β-ACTIN antibody, Filipin and 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) were purchased from Sigma-Aldrich/ Merck Millipore. Alexa488-conjugated anti-rabbit IgG, HRP-conjugated anti-rabbit total IgG and light chain specific IgG antibodies were purchased from Jackson ImmunoResearch, USA; PE-conjugated F4/80 was procured from Tonbo Biosciences, USA. Alexa Fluor 660 conjugated CD68 was purchased from ThermoFischer Scientific. Anti-G9a, anti-SIRT6, anti-H3K9me1, anti-H3K9me2, anti-H3K9Ac, anti-Ser33/37/Thr41 phospho-β-CATENIN, anti-Ser9 phospho-GSK-3β, anti-β-CATENIN, anti-NRF2, anti-HO1 and anti-TRXR1 antibodies were obtained from Cell Signaling Technology, USA. Anti-LRP2 antibody was purchased from Santa Cruz Biotechnology, USA; anti-SREBP2 antibody was procured from Abcam, USA; and anti-NQO1 antibody was purchased from Calbiochem, USA.

Prakhar P., Bhatt B., Lohia G.K., Shah A., Mukherjee T., Kolthur-Seetharam U., Sundaresan N.R., Rajmani R.S, & Balaji K.N. (2023). G9a and Sirtuin6 epigenetically modulate host cholesterol accumulation to facilitate mycobacterial survival. PLOS Pathogens, 19(10), e1011731.

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Quantitative Analysis of Extracellular Matrix Proteins

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Western blot analysis was performed as described previously [24 (link)]. Detection of secreted fibronectin was performed as described previously [25 (link)]. Each group included five samples. Primary antibodies used in this study were anti-G9a (Cell Signaling Technology, Danvers, MA, USA), anti-α-SMA (Sigma-Aldrich), anti-zonula occludens-1 (ZO-1; Invitrogen, Carlsbad, CA, USA), anti-fibronectin (Sigma-Aldrich), anti-α-tubulin (Sigma-Aldrich), anti-H3K9me1 (Cell Signaling Technology), and anti-H3 (Cell Signaling Technology). The intensity of each band was determined using ImageJ software. Concentrations of TGF-β1 in peritoneal fluid were measured with an ELISA (R&D Systems), following the manufacturer’s instructions.

Maeda K., Doi S., Nakashima A., Nagai T., Irifuku T., Ueno T, & Masaki T. (2017). Inhibition of H3K9 methyltransferase G9a ameliorates methylglyoxal-induced peritoneal fibrosis. PLoS ONE, 12(3), e0173706.

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Western Blot Analysis of Histone Modifications

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Protein samples were resolved by SDS-PAGE and transferred to nitrocellulose membranes. The membranes were blocked in 3% BSA buffer (10 mM Tris-HCl, pH7.4, 150 mM NaCl, 1 mM EDTA, 0.1% Tween-20 and 3% BSA). Primary antibodies used for western blotting were: anti-H3K9me1 (Cell Signaling, #14186), anti-H3K9me2 (Cell Signaling, #4658), anti-H3K9me3 (Wako Diagnostics/Chemicals, 309–34839), anti-H3K27me1 (Cell Signaling, #7693), anti-H3K27me2 (EMD Millipore, #04–821), anti-H3K27me3 (Abcam, ab6002), anti-total H3 (EMD Millipore, 06–755), anti-V5 (Abcam, ab27671), anti-phospho ERK1/2 (Cell Signaling, #4370), anti-ERK1/2 (Cell Signaling, #4695), anti-G9a (Cell Signaling, #3306), anti-GLP (Abcam, ab135487), anti-LC3B (Cell Signaling, #3868), anti-MITF (C5, in-house), anti-active β-catenin (Cell Signaling, #8814), β-catenin (Cell Signaling, #9587), anti-DKK1 (Santa Cruz, sc-374574), anti-β-actin (Santa Cruz, sc-47778), anti-α-tubilin (Sigma Aldrich, T9026), anti-Lamin A/C (Cell Signaling, #4777) and anti-Lamin B (Cell Signaling, #12586). Appropriate secondary antibodies were used in 5% skim milk/TBST buffer. Protein bands were visualized using Western lightning plus ECL (Perkin Elmer) and quantified using ImageJ software.

Kato S., Weng Q.Y., Insco M.L., Chen K.Y., Muralidhar S., Poźniak J., Diaz J.M., Drier Y., Nguyen N., Lo J.A., van Rooijen E., Kemeny L.V., Zhan Y., Feng Y., Silkworth W., Powell C.T., Liau B.B., Xiong Y., Jin J., Newton-Bishop J., Zon L.I., Bernstein B.E, & Fisher D.E. (2020). Gain-of-function genetic alterations of G9a drive oncogenesis. Cancer discovery, 10(7), 980-997.

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Immunostaining for Cell Signaling

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HRP-conjugated anti-β-ACTIN antibody, Filipin and 4′,6-Diamidino-2phenylindole dihydrochloride (DAPI) were purchased from Sigma-Aldrich/ Merck Millipore. Alexa488-conjugated anti-rabbit IgG, HRP-conjugated anti-rabbit total IgG and light chain specific IgG antibodies were purchased from Jackson ImmunoResearch, USA; PE-conjugated F4/80 was procured from Tonbo Biosciences, USA. Anti-G9a, anti-SIRT6, anti-H3K9me1, anti-H3K9me2, anti-H3K9Ac, anti-Ser33/37/Thr41 phospho-β-CATENIN, anti-Ser9 phospho-GSK-3β, anti-β-CATENIN, anti-NRF2, anti-HO1 and anti-TRXR1 antibodies were obtained from Cell Signaling Technology, USA. Anti-LRP2 antibody was purchased from Santa Cruz Biotechnology, USA; anti-SREBP2 antibody was procured from Abcam, USA; and anti-NQO1 antibody was purchased from Calbiochem, USA.

Prakhar P., Bhatt B., Mukherjee T., Lohia G.K., Kolthur‐Seetharam U., Sundaresan N.R., Rajmani R.S., & Balaji K.N. (2021). G9a and Sirtuin6 epigenetically modulate host cholesterol accumulation to facilitate mycobacterial survival.

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