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High sensitivity rna screentape analysis kit

Manufactured by Agilent Technologies
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The High Sensitivity RNA Screentape analysis kit is a lab equipment product designed for the analysis of RNA samples. It provides a rapid and accurate method for the quantification and quality assessment of RNA in a sample.

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Lab products found in correlation

Tapestation 4200, by Agilent Technologies (4 mentions) Nanodrop 1000 spectrophotometer, by Thermo Fisher Scientific (4 mentions) Mirneasy kit, by Qiagen (3 mentions) Steponeplus, by Thermo Fisher Scientific (2 mentions) Power sybr green master mix, by Thermo Fisher Scientific (1 mentions) Nextseq 500, by Illumina (1 mentions) Smart seq v4 ultra low input rna kit, by Takara Bio (1 mentions) Agilent 4200 tapestation system, by Agilent Technologies (1 mentions) Nextera xt dna library preparation kit, by Illumina (1 mentions) Agilent 4200 tapestation, by Agilent Technologies (1 mentions) Single cell rna purification kit, by Norgen Biotek (1 mentions)

Spelling variants of this product

RNA ScreenTape (130 citations) High Sensitivity RNA ScreenTape (57 citations) RNA Analysis ScreenTape (14 citations) High Sensitivity RNA Analysis Kit (10 citations) High Sensitivity RNA ScreenTape Analysis (6 citations) RNA ScreenTape Analysis kit (4 citations)

5 protocols using high sensitivity rna screentape analysis kit

1

Quantification of RNA and Small RNA Expression

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Total RNA from primary chondrocytes and TC28/I2 cells was isolated using MiRNeasy Kit (Qiagen, Cat # 217004) as described previously [18 (link)]. The Nanodrop 1000 Spectrophotometer (Thermo Fisher) was used to quantify RNA and RNA integrity was determined using the TapeStation 4200 (Agilent Technologies) with High Sensitivity RNA Screentape analysis kit (Agilent, Cat # 50675579). All qPCR reactions were conducted using TaqMan or Sybr Green assays with either TaqMan master mix (IDT, Cat # 1055770) or Power Sybr Green master mix (Life technologies, Cat # 4367659) on a Step One Plus (Applied Biosystems) machine. Target mRNA levels were determined as fold change differences following the delta-delta Ct method with β-Actin employed as the endogenous control for normalization. Target tRNA and tRF levels were determined as fold change differences following the delta-delta Ct method with RNU6, SNORD43 and SNORD 45 employed as the endogenous control for normalization.
Green J.A., Ansari M.Y., Ball H.C, & Haqqi T.M. (2020). tRNA-Derived Fragments (tRFs) Regulate Post-Transcriptional Gene Expression via AGO-Dependent Mechanism In IL-1β Stimulated Chondrocytes. Osteoarthritis and cartilage, 28(8), 1102-1110.
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2

Single-cell RNA-seq of Tumor-associated Monocytes

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RNA was purified from FACS sorted MN/MCA1 tumor-infiltrating monocytes and TAMs deriving from wt and C3−/− mice (4 biological replicates each) with Single Cell RNA Purification Kit (Norgen Biotek Corp.). RNA quality control was performed with Agilent 4200 Tape Station system using High Sensitivity RNA ScreenTape analysis kit (Agilent, Santa Clara, CA, USA). Only RNAs having a RIN>7.5 were used for library preparation. Libraries were prepared starting from 5 ng totRNA for each sample by using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara), based on Clontech’s proprietary SMART® (Switching Mechanism at 5’ End of RNA Template) technology. Full-length cDNAs were processed with Nextera XT DNA Library Preparation Kits (Illumina, San Diego, CA, USA). Libraries were checked using Agilent TapeStation 4200 using High Sensitivity DNA ScreenTape analysis kit. Samples were sequenced on Illumina NextSeq 500 at an average of 29,246,059 bases-long, single-end reads; among these an average of 26,000,253 reads were uniquely mapped.
Magrini E., Di Marco S., Mapelli S.N., Perucchini C., Pasqualini F., Donato A., de la Luz Guevara Lopez M., Carriero R., Ponzetta A., Colombo P., Cananzi F., Supino D., Reis E.S., Peano C., Inforzato A., Jaillon S., Doni A., Lambris J.D., Mantovani A, & Garlanda C. (2021). Complement activation promoted by the lectin pathway mediates C3aR-dependent sarcoma progression and immunosuppression. Nature cancer, 2(2), 218-232.
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3

RNA Extraction from OA Cartilage Samples

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De-identified human OA cartilage approved by Northeast Ohio Medical University (NEOMED) Institutional Review Board and Summa Health Systems, Barberton, Ohio as ‘non-human subject study under 45 CFR’ was used. Total RNA was extracted from smooth, macroscopically intact human OA cartilage with a Mankin score of 2 or less (n = 1, female, 60 years old) and damaged OA cartilage with a Mankin score of 4 or higher (n = 1, female, 80 years old) using MiRNeasy Kit (Qiagen, Germantown, MD, USA). RNA was quantified using the Nanodrop 1000 Spectrophotometer (Thermo Fisher, Waltham, MA, USA). TapeStation 4200 (Agilent Technologies, Santa Clara, CA, USA) was used to determine RNA integrity using High Sensitivity RNA Screentape analysis kit (Agilent, Santa Clara, CA, USA).
Balaskas P., Green J.A., Haqqi T.M., Dyer P., Kharaz Y.A., Fang Y., Liu X., Welting T.J, & Peffers M.J. (2020). Small Non-Coding RNAome of Ageing Chondrocytes. International Journal of Molecular Sciences, 21(16), 5675.
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4

Isolating and Quantifying RNA from Chondrocytes

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Total RNA from cultured chondrocytes and other cell types was isolated using a modified Trizol-Chloroform method described earlier22 (link). RNA was quantified using a Nanodrop 1000 Spectrophotometer (Thermo Fisher) and the integrity of the RNA preparation was determined using the TapeStation 4200 (Agilent Technologies) with High Sensitivity RNA ScreenTape analysis kit (Agilent, Cat #5067–5579). cDNA was synthesized using the High Capacity kit as described above. qPCR reactions were conducted using TaqMan assays with TaqMan master mix (IDT, Cat #1055770) on a StepOne Plus (Applied Biosystems) machine. Target mRNA levels were determined as fold change differences following the delta-delta Ct method with β-Actin expression level employed as the endogenous control for normalization.
Ball H.C., Ansari M.Y., Ahmad N., Novak K, & Haqqi T.M. (2020). A Retrotransposon gag-like-3 gene RTL3 and SOX-9 Co-regulate the Expression of COL2A1 in Chondrocytes. Connective tissue research, 62(6), 615-628.
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5

RNA Extraction from Healthy and Damaged OA Cartilage

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De-identified human OA cartilage approved by Northeast Ohio Medical University (NEOMED) Institutional Review Board and Summa Health Systems, Barberton, Ohio as 'non-human subject study under 45 CFR' was used. Total RNA was extracted from smooth, macroscopically intact human OA cartilage with a Mankin score of 2 or less (n=1, female, 60 years old) and damaged OA cartilage with a Mankin score of 4 or higher (n=1, female, 80 years old) using MiRNeasy Kit (Qiagen, Germantown, USA). RNA was quantified using the Nanodrop 1000 Spectrophotometer (Thermo Fisher, USA). TapeStation 4200 (Agilent Technologies, USA) was used to determine RNA integrity using High Sensitivity RNA Screentape analysis kit (Agilent, USA).
Balaskas P., Green J.A., Haqqi T.M., Dyer P., Kharaz Y.A., Fang Y., Liu X., Welting T.J.M., & Peffers M.J. (2020). Small Non-coding RNAome of ageing chondrocytes.
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