RAW 264.7 cells (5–105 cells per well) were incubated for 24 hours in the absence and presence of 0.5 µM MBP C8 or MBP C1 isomers. In other series of experiments, RAW 264.7 cells were treated with cytokines to induce either M1 or M2 cell state with or without 0.5 µM MBP charge isomers. For M1 polarization, cells were treated for 24 hours with and without 0.5 µM MBP charge isomers in the presence of 20 ng/mL of interferon-gamma (IFN-γ) (ab123747; Abcam, Cambridge, UK) and 100 ng/mL of lipopolysaccharide (LPS) (L2880-100MG; Sigma-Aldrich Co.); for M2 polarization, in the presence of 20 ng/mL of IL-4 (ab191628; Abcam) and 10 ng/mL of IL-10 (BMS347; eBioscience, Vienna, Austria). Cell viability was assessed by staining the cells with Trypan blue (#1450021; Bio-Rad, Hercules, CA, USA) using an automated Cell Counter TC 20™ (Bio-Rad). The cells were then harvested using Cell Scraper (C6106-100EA; Greiner Bio One, Frickenhausen Germany), and the harvested cells were maintained in growth medium and used for further analysis.
Cell counter tc 20
Manufactured by Bio-Rad
Sourced in United States
The TC 20™ Cell Counter is a compact, automated cell counting device designed for accurate and efficient cell enumeration. It utilizes advanced optics and image analysis algorithms to rapidly determine the cell count and viability of a sample. The TC 20™ provides a reliable and consistent means of cell quantification, making it a useful tool for various applications in cell biology and research.
Automatically generated - may contain errors
Lab products found in correlation
Cho s, by Thermo Fisher Scientific (2 mentions)
Trypan blue, by Bio-Rad (1 mentions)
Cell scraper, by Greiner (1 mentions)
L2880 100mg, by Merck Group (1 mentions)
Trypan blue solution, by Nacalai Tesque (1 mentions)
Ham s f12, by Fujifilm (1 mentions)
Penicillin streptomycin solution, by Nacalai Tesque (1 mentions)
Co2 incubator, by Panasonic (1 mentions)
3 protocols using cell counter tc 20
1
Modulation of Macrophage Polarization by MBP Isomers
2
Quantifying NK Cell Proliferation
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NK cells from each culture of each subset were counted on days 6, 10, and 11 after sorting using cell counter TC20 (Bio-Rad Laboratories, Hercules, CA, USA). Transduced cells were counted on days 4 and 10 after the transduction. Proliferative capacity was determined by the expansion coefficient, calculated using the following formula: K = number of cells on day X/number of cells on day 0.
3
Culturing CHO Cells for Suspension
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Chinese hamster ovary cells for suspension culture (CHO-S) were purchased from Gibco, USA. CHO 1-15 cells (CRL-9606) were purchased from ATCC, USA. Ham's F-12 (Wako Pure Chemical Corporation, Japan) with 10% FBS (Thermo Fisher Scientific, USA) and CHO-S-SFM II (Thermo Fisher Scientific, USA) were used for culture of CHO-S and CHO 1-15 cells, respectively. All media were supplemented with penicillin/streptomycin solution (Nacalai Tesque, Japan). Cells were maintained in CO 2 incubator (Panasonic, Japan), at 37 °C with air containing 5% CO 2 . Cell number was measured by Cell Counter TC20 (Bio-Rad, USA) after staining cells with 0.5% trypan blue solution (Nacalai Tesque, Japan).
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