Unity inova spectrometer

Composition of the E3 fraction was investigated by high-resolution Liquid Chromatography-Mass Spectroscopy (LC–MS) using a Thermo LTQ-Orbitrap XL system, and by 1D (¹H and ¹³C) and 2D (COSY, HSQC and HMBC) NMR using a Varian UnityInova spectrometer at 700 MHz equipped with a cryogenic probe. NMR chemical shifts were referenced to the residual solvent signal (CD₃OD: δ_C 49.0, δ_H 3.31; pyridine-d₅ δ_C 150.3, 135.9 and 123.9, δ_H 8.73, 7.56 and 7.21).

MR imaging was performed using a Varian 4.7T magnet with a UnityInova spectrometer. A birdcage volume coil (72 mm inner diameter) was used for both transmitting and receiving. An animal monitoring system (Small Animal Instruments Inc., Stony Brook, NY) enabled image acquisition to be gated to ECG and respiration. Throughout imaging, the core body temperature of the animal was maintained at 35–38°C by directing a regulated stream of warm air over the animal, and both heart rate and body temperature were recorded.
Cine MR images at 3 long‐axis (angular spacing = 60°) and 6 short‐axis slices (spacing = 0.6 mm–1.0 mm) were acquired to ensure full coverage of the LV. Each slice was imaged at 18 evenly spaced time‐points through the cardiac cycle. T1‐weighted gradient‐echo cine acquisitions used the following parameters: repetition time, TR = 2 ×  R‐R interval, ~ 280 ms ‐ 360 msec; echo time, TE = 2.2 msec; cardiac phases = 20; flip angle = 20°; slice thickness = 2 mm; averages = 2, field of view = 60 mm × 60 mm; matrix = 128 pixels × 128 pixels; gap between slices = 0.6 mm–1.0 mm according to the size of the heart.

Elemental analyses were performed using a Perkin-Elmer 240CHN elemental analyzer (PerkinElmer Japan Co., Ltd., Tokyo, Japan). Mass spectra were recorded by a JEOL JMS-SX 102 AQQ mass spectrometer (JEOL Ltd., Tokyo, Japan). ¹H- and ¹³C-NMR were measured using a Varian Unity Inova spectrometer (Varian, Inc., Palo Alto, CA, USA), with tetramethylsilane as an internal standard.

After removing aliquots for fluorescence microscopy, the remainder of the sample was transferred to a 1.5 mL microcentrifuge tube and freeze-dried. The solids remaining in the tube were then dissolved in an NMR solvent. The proton NMR spectra were acquired in dimethyl sulfoxide-d₆ or methanol-d₄ at 25 °C on a 500 MHz Varian Unity/Inova spectrometer.

A 700 MHz Varian Unity INOVA spectrometer was employed to perform the NMR experiments. One-dimensional proton spectra were recorded at 7°C using pulsed-field gradient DPFGSE for water suppression. All DNA samples were prepared at 0.2 mM strand concentration in 0.22 mL (H₂O/D₂O 9:1) buffer solution. DNA/ligand mixtures were obtained by adding aliquots of a stock solution of the six ligands in DMSO-d6 directly to the DNA solution inside the NMR tube (Randazzo et al., 2002 (link); Amato et al., 2017 (link)). NMR data were processed using the iNMR software (www.inmr.net).