Plasma concentrations of syndecan-1, hyaluronic acid and thrombomodulin were measured using commercial ELISA-kits following the manufacturer’s instructions. Syndecan-1 was analyzed with kits from Diaclone (Human CD138 ELISA kit, catalogue number 851.620.001). Hyaluronic acid and thrombomodulin were analyzed with kits from R&D-systems (hyaluronic acid: Hyaluronan Quantikine ELISA kit catalogue number DHYAL0 and thrombomodulin: Human Thrombomodulin/BDCA-3 Quantikine ELISA Kit catalogue number DTHBD0). Calibrators and samples were run in duplicates. A quality control (QC) sample was run in duplicates on every plate. For syndecan-1, 19 samples were undiluted, 30 diluted 1:12, and eight were diluted 1:32. For analyses of hylaruronic acid and thrombomodulin samples were diluted 1:10. Absorbance was read with a Multiscan GO instrument, using SkanIT software 4.1. All data were double checked for accuracy.
Human thrombomodulin bdca 3 quantikine elisa kit
Manufactured by R&D Systems
Sourced in United States
The Human Thrombomodulin/BDCA-3 Quantikine ELISA Kit is a quantitative sandwich enzyme immunoassay designed to measure human thrombomodulin/BDCA-3 levels in cell culture supernates, serum, and plasma. The assay employs a specific antibody pre-coated onto a microplate.
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Lab products found in correlation
Hyaluronan quantikine elisa kit, by R&D Systems (1 mentions)
Human cd138 elisa kit, by Diaclone (1 mentions)
Tnf α elisa kit, by BioLegend (1 mentions)
Human d dimer elisa kit, by Abcam (1 mentions)
Human thrombin antithrombin complex elisa kit, by Abcam (1 mentions)
Human adamts13 quantikine elisa kit, by R&D Systems (1 mentions)
Human cd62p elisa kit, by Abcam (1 mentions)
Human coagulation factor 3 tissue factor quantikine elisa kit, by R&D Systems (1 mentions)
Human serpin e1 pai 1 quantikine elisa kit, by R&D Systems (1 mentions)
Human hgf quantikine elisa kit, by R&D Systems (1 mentions)
Zymutest pai 1 antigen, by Hyphen Biomed (1 mentions)
Zymutest t pa antigen, by Hyphen Biomed (1 mentions)
Human se selectin cd62e quantikine elisa kit, by R&D Systems (1 mentions)
Hemosil von willebrand factor antigen, by Werfen (1 mentions)
4 protocols using human thrombomodulin bdca 3 quantikine elisa kit
1
Plasma Biomarkers of Endothelial Dysfunction
2
Quantifying Liver TNF-α and Thrombomodulin
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The TNF-α and rhsTM levels in the homogenised liver were measured using the TNF-α ELISA Kit (BioLegend, San Diego, USA) and Human Thrombomodulin/BDCA-3 Quantikine ELISA Kit (R&D Systems, Minnesota, USA) according to the manufacturer’s instructions.
3
Quantifying Plasma Coagulation Markers
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We assessed plasma samples by using commercially available kits. For plasminogen activator inhibitor 1 (PAI-1) we used Human Serpin E1/PAI-1 Quantikine ELISA Kit (R&D Systems, https://www.rndsystems.com ) or Human PAI-1 Platinum Kit (eBioscience, https://www.thermofisher.com ). We used Human t-Plasminogen Activator/tPA Quantikine ELISA Kit (R&D Systems) to measure tissue plasminogen activator (tPA). We used Human Thrombomodulin/BDCA-3 Quantikine ELISA Kit (R&D Systems) or Human Thrombomodulin ELISA Kit (Innovative Research Inc., https://www.innov-research.com ) to measure thrombomodulin (THBD) and Human Thrombin-Antithrombin Complex ELISA Kit (Abcam, https://www.abcam.com ) to measure thrombinantithrombin (TAT) complexes. To assess endothelial protein C receptor (EPCR) we used Human EPCR DuoSet ELISA Kit (R&D Systems), for D-dimer we used Human D-Dimer ELISA Kit (Abcam), for a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13 (ADAMTS-13) we used Human ADAMTS13 Quantikine ELISA Kit (R&D Systems), for P-selectin we used Human CD62P ELISA Kit (Abcam), for hepatocyte growth factor (HGF) we used Human HGF Quantikine ELISA Kit (R&D Systems), for von Willebrand factor (vWF) we used vWF Human ELISA Kit (ThermoFisher, https://www.thermofisher.com ), and for tissue factor we used Human Coagulation Factor III/Tissue Factor Quantikine ELISA Kit (R&D Systems).
4
Plasma Biomarkers of Endothelial Function
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Plasma levels of von Willebrand factor (vWF) antigen were measured using a commercial method (HemosIL Von Willebrand Factor Antigen, Instrumentation Laboratory, Bedford, MA, USA) with intra- and inter-assay CVs of <2.3%; the lower detection limit was 2.2%.
Tissue-type plasminogen activator (t-PA) antigen was measured in plasma using a commercially available ELISA (Zymutest t-PA antigen, Hyphen BioMed, Neuville sur Oise, France) in accordance with the manufacturer's instructions. The intra- and inter-assay CVs were <10%, and the lower detection limit was 0.5 ng/mL.
Plasminogen activator inhibitor-1 (PAI-1) antigen was measured in plasma using a commercially available ELISA (Zymutest PAI-1 antigen, Hyphen BioMed) whose intra- and inter-assay CVs were 8% and 13%, respectively; the lower detection limit was 0.5 ng/mL.
Soluble plasma thrombomodulin (sTM) plasma levels were measured using a commercial sandwich ELISA (Human Thrombomodulin/BDCA-3 Quantikine ELISA Kit, R&D Systems, Minneapolis, MN, USA) whose intra- and inter-assay CVs were both <10%; the lower detection limit was 7.82 pg/mL.
Soluble endothelial selectin (sE-selectin) was measured in plasma using a sandwich ELISA (Human sE-Selectin/CD62E Quantikine ELISA Kit, R&D Systems Inc.) whose intra- and inter-assay CVs were respectively 5% and 8.8%; the lower detection limit was 0.009 ng/mL.
Tissue-type plasminogen activator (t-PA) antigen was measured in plasma using a commercially available ELISA (Zymutest t-PA antigen, Hyphen BioMed, Neuville sur Oise, France) in accordance with the manufacturer's instructions. The intra- and inter-assay CVs were <10%, and the lower detection limit was 0.5 ng/mL.
Plasminogen activator inhibitor-1 (PAI-1) antigen was measured in plasma using a commercially available ELISA (Zymutest PAI-1 antigen, Hyphen BioMed) whose intra- and inter-assay CVs were 8% and 13%, respectively; the lower detection limit was 0.5 ng/mL.
Soluble plasma thrombomodulin (sTM) plasma levels were measured using a commercial sandwich ELISA (Human Thrombomodulin/BDCA-3 Quantikine ELISA Kit, R&D Systems, Minneapolis, MN, USA) whose intra- and inter-assay CVs were both <10%; the lower detection limit was 7.82 pg/mL.
Soluble endothelial selectin (sE-selectin) was measured in plasma using a sandwich ELISA (Human sE-Selectin/CD62E Quantikine ELISA Kit, R&D Systems Inc.) whose intra- and inter-assay CVs were respectively 5% and 8.8%; the lower detection limit was 0.009 ng/mL.
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