Human ifn β elisa kit

Virus-inducible cytokines production in ALICE_im. pYW274/pYW365-transgenic cells were incubated with HCV (MOI = 3), HBV [1000 virion genome equivalents (vge)/cell], ADV (MOI = 10), HSV-1 (MOI = 5) and Vehicle (equal volume of DMEM) as control for 24 h. The IFN-α/IFN-β production in culture supernatants was quantified by ELISA at 2/4 dpi, using a human IFN-α ELISA kit (Beyotime, cat. no. PI505) or human IFN-β ELISA kit (Beyotime, cat. no. PI572) according to manufacturer’s instructions.
qPCR analysis of the viral transcript genes in ALICE_im. pYW274/pWS67-transgenic cells (ALICE_sen-SEAP), pYW274/pYW365-transgenic cells (ALICE_im-IFNα) or pYW274/pYW327-transgenic cells (ALICE_im-IFNβ) were incubated with the HCV (MOI = 3), HBV (1000 vge/cell), ADV (MOI = 10), HSV-1 (MOI = 5) for 24 h. The relative viral mRNA expression was quantified by qPCR at 2/4 dpi. All data were normalized to the viral gene expression levels in ALICE_sen-SEAP control group infected with the corresponding viruses. All primers used are provided in Supplementary Table 2.

IR780, HSA, zinc acetate, and sodium sulfide were purchased from Solarbio (Beijing, China). Fetal bovine serum (FBS) and Dulbecco’s modified Eagle medium (DMEM) were purchased from GIBCO (Grand Island, NY, USA). Mito-Tracker Green was purchased from Beyotime (Shanghai, China; #C1048). Annexin V-FITC Apoptosis Kit (BD, San Jose, CA, USA; #556557) was from Becton, Dickinson and Company (Franklin Lakes, NJ, USA). Anti-DFNA5/GSDME-N-terminal (Abcam, Cambridge, UK; #AB215191), anti-calreticulin antibody (Abcam, #ab92516), anti-high-mobility group box 1 (HMGB1) antibody (Abcam, #ab79823), anti-calreticulin antibody (Abcam, ab92516), goat anti-rabbit IgG H&L (Alexa Fluor 488) (Abcam, #ab150081), anti-CD8 antibody (Abcam, #ab217344), anti-FOXP3 (Abcam, #ab20034) were from Abcam. Cleaved caspase-3 (CST, Danvers, MA, USA; #9664T), cGAS (CST, #31659S), STING (CST, #50494S), and p-STING (CST, #50907) were from Cell Signaling Technology (Danvers, MA, USA). Atezolizumab (Selleck, Munich, Germany; #A2004) were from Selleck. Mouse IFN-β ELISA Kit (Beyotime, #PI568) and human IFN-β ELISA Kit (Beyotime, #PI572) were from Beyotime. SOSG9 (Meilunbio, Dalian, China; #MA0326) was from Meilunbio.