Masson trichrome staining reagent

Fetal Bovine Serum (Bio-Channel, BC-SE-FBS07), DMEM/F-12 (BiologicalIndustries, 01-170-1A), EDTA-Trypsin (Biosharp, BL512A), EndoFectin™Max Reagent (GeneCopoeia, EF013), Cell Value Added-Toxicity Assay Kit (Biosharp, BS350B), PA (Aladdin, S161420), OA (Maclean, S817542), ORO Staining Kit (Solarbio, G1262), BlazeTaq™ SYBR®Green Mix (GeneCopoeia, QP031), Dnase QP031), BCA Protein Reagent (P0009-1, P0009-1), Hematoxylin (Solarbio, H8070), Eosin Staining Reagent (Beyotime, C0105-2), Masson Trichrome Staining Reagent (Solarbio, G1340), TRIzol®Reagent (Life, Cat. no. 15596-018), SureScript™ First-Strand cDNA Synthesis Kit (GeneCopoeia, QP056), Immobilon Western HRP Substrate Luminol Reagent (Affinity, KF001), 30% Acrylamide/Bis solution (29:1) (Solarbio, A1010), 24-well plate (VIRYA, 3512409), FBS(Bio-Channel, BC-SE-FBS07), Hieff® Quick exosome isolation kit Plus (YEASON, China).
Tissue embedding kit (Jiangsu Shitai, 20084), Real-timePCR (Thermo Fisher Scientific, TCR0096, USA), UV spectrophotometer (ALLSHENG, USA), benchtop low-speed centrifuge (Weil Ltd., China), inverted fluorescence microscope (Zeiss, Observer.A1, Germany), gel imager (Monad, USA), ice maker (FM150KE, China), 4 °C refrigerator (Haier, China), baking machine (Thermo Scientific, DB-B2, USA), TEM(ZCIBIO, China), NTA(Particle Metrix, China).

Antibodies against S100A16, GRP78, IRE1α, XPB1, Fibronectin, and α-SMA for western blot analysis, GRP78 antibody for IHC, and Co-IP studies. The antibodies against β-actin and GAPDH were purchased from Proteintech (Chicago, IL, USA). The S100A16 antibody used for IHC, IF, and Co-IP studies were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). The S100A16 and GRP78 antibodies for IF staining were purchased from Abcam (Cambridge, MA, USA). Protein A-agarose beads were purchased from EMD Millipore Corp (St. Louis, MO, USA). The antibodies of ubiquitin, p-IRE1α, EIF2α, p-EIF2α, p-PERK, PERK ATF6, and CHOP were purchased from Cell Signaling Technology (Billerica, MA, USA). The mouse IgG for Co-IP and secondary antibodies for western blotting and ER Tracker Blue-White were purchased from Thermo Fisher Scientific (Waltham, UK). Masson trichrome staining reagent was purchased from Solarbio (Beijing, China). Recombinant human TGF-β1 was purchased from PEPROTECH (Rocky Hill, NJ, USA). BAPTA-AM was purchased from MCE (New Jersey, USA). S100A16 overexpression lentivirus and negative control virus were purchased from Gene (Shanghai, China). Puromycin dihydrochloride and liposomal transfection reagent were purchased from YEASEN (Shanghai, China).