Elecsys anti tg

TRAb (Elecsys Anti-TRAb v2, Roche Diagnostics; reference range, <2.0 IU/L), TgAb (Elecsys Anti-Tg, Roche Diagnostics; reference range, <28.0 IU/mL), and TPOAb (Elecsys Anti-TPO, Roche Diagnostics; reference range, <16.0 IU/mL) were measured on a Roche Cobas e801 analyzer (Roche Diagnostics) according to the manufacturer’s instructions. TSH (Elecsys TSH v2, Roche Diagnostics; reference range, 0.500-5.000 μIU/mL), free T4 (FT4, Elecsys FT4III, Roche Diagnostics; reference range, 0.90-1.7 ng/dL), and free T3 (FT3, Elecsys FT3III, Roche Diagnostics; reference range, 2.30-4.00 pg/mL) were measured on a Roche Cobas e801 analyzer (Roche Diagnostics) according to the manufacturer’s instructions. Increases of TRAb, TSH, FT4, and FT3 from baseline to 4 weeks after the third dose were defined as ΔTRAb, ΔTSH, ΔFT4, and ΔFT3, respectively, which were calculated as:
(value at 4 weeks after the third dose) - (value at baseline).
Increases of TgAb and TPOAb from 32 weeks after the second dose (pre-third dose) to 4 weeks after the third dose (post-third dose) were also defined as ΔTgAb and ΔTPOAb, respectively, and calculated as:
(vales at 4 weeks after the third dose) - (values at 32 weeks after the second dose).
Responders to increase in TRAb were defined as the subjects who exhibited increase in TRAb in a time dependent manner and had TRAb >1.2 IU/L at 4 weeks after the third dose.

Patients’ follow-ups included thyroid and neck ultrasonography examinations and assessment of serum TSH and thyroglobulin (Tg) and anti-thyroglobulin antibodies (TgAb) levels every 6 months. All patients had to follow-up for at least 6 months.
Patients with posterior tumors near the trachea or esophagus or located along the course of the recurrent laryngeal nerves or carotid were excluded.
Prospective active surveillance was considered when diagnosis of PTC was made at the beginning of follow-up and retrospectively when cytological diagnosis of Bethesda categories V and VI was established after surveillance of a single thyroid nodule that grew since a previous ultrasonographic follow-up.
Serum Tg levels were measured by an electrochemiluminescent method (ECLIA), Elecsys 2010 (Roche) with an analytical sensitivity of 0.04 ng/mL and functional sensitivity of 0.1 ng/mL. TgAb levels were measured by an electrochemiluminescent method, Elecsys Anti-Tg (Roche); values > 20 IU/mL were considered positive. Serum TSH was measured with a commercialized chemiluminescence assay kit (Siemens Advia Centaur^® XPT TSH3-UL) (reference range of 0.35 to 5.5 mU/l).
Additionally, the same operator performed a neck ultrasound with a linear 11 MHz transducer every six months.