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Ff01 452 45

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The FF01-452/45 is a laboratory equipment product manufactured by IDEX Corporation. It serves as a core function to facilitate specific tasks or processes in a laboratory setting. The detailed specifications and intended use of this product are not included in this factual and unbiased description.

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Lab products found in correlation

H7422 40 gaasp, by Hamamatsu Photonics (2 mentions) 1.0 na water immersion objective, by Olympus (2 mentions) Ff520 di02, by IDEX Corporation (2 mentions) Ff506, by IDEX Corporation (1 mentions) Ff01 609 181, by IDEX Corporation (1 mentions) Dg10 1500, by Thorlabs (1 mentions) Ff510 di02, by IDEX Corporation (1 mentions) Orca 05g, by Hamamatsu Photonics (1 mentions)

5 protocols using ff01 452 45

1

Intravital Imaging of Somatosensory Cortex

Cited 2 times
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Live animal imaging was performed as previously described 7 (link), 33 (link), 56 (link). Briefly, a Sutter Movable Objective Microscope (MOM) equipped with a pulsed femtosecond Ti:Sapphire laser (Chameleon Ultra II, Coherent) with two fluorescence detection channels was used for imaging (dichroic beamsplitter: FF520-Di02 (Semrock); blue emission filter: FF01-452/45 (Semrock); green emission filter: ET525/70M (Chroma); photomultiplier tubes: H7422-40 GaAsP (Hamamatsu)). Laser excitation wavelength was set to 830nm. Average laser power was <10-15mW at the tissue surface and adjusted with depth as needed to compensate for signal loss due to scattering and absorption. An Olympus 20× 1.0-NA water immersion objective was used for light delivery and collection. Z-stacks included up to 350 images, acquired at 1μm axial step size, used a 2-frame average, 512 × 512 pixel resolution, and 2.0x-10x zoom (corresponding to 350μm-72μm fields of view). Time-lapse recordings typically included 60–70 images/stack, acquired at 1.0–1.2 μm axial step size, used a 2-frame average, 60 stack repeats (corresponding to approximately 94 min total recording duration), 512 × 512 pixel resolution, and 3.3x-5x zoom (corresponding to 212 μm - 142 μm fields of view). Up to thirteen z-stacks, and four to seven time-lapse recordings were acquired per animal in layers 1 and 2 of the somatosensory cortex.
Huang Y., Happonen K.E., Burrola P.G., O’Connor C., Hah N., Huang L., Nimmerjahn A, & Lemke G. (2021). Microglia use TAM receptors to detect and engulf amyloid beta plaques. Nature immunology, 22(5), 586-594.
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2

Optical Filter Design and Fabrication

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The optical filter was designed using Solidworks and CNC-machined in aluminium to incorporate an LED (ASMT-BB20, USA, Avago Technologies), a convex lens (45117, Edmund Optics), achromatic lens (AC060-010-A, Thorlabs), an excitation filter (FF01-452/45, Semrock, USA), single-edge dichroic beam-splitter (FF506, Semrock, USA), and a fluorescence emission filter (FF01-534/42, Semrock, USA).
Al-Rawhani M.A., Beeley J, & Cumming D.R. (2015). Wireless fluorescence capsule for endoscopy using single photon-based detection. Scientific Reports, 5, 18591.
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3

Cell Morphology Imaging in Acute Brain Slices

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For screening cells morphology and checking cell localization in acute brain slices we used a widefield infrared illumination system. This consisted of an IR-LED source (M780L2, Thorlabs) installed at the rear port of a SliceScope Scientifica microscope, an orientable blocking element to create oblique illumination and a condenser focusing the light on the sample. IR light transmitted through the sample was collected with an IR antireflection coated water-immersion objective (Nikon NIR MRD07420 N40X/0.80W) and sent to an IR CCD (IR-1000, DAGE-MIT).
For a first control of ReaChR expression, we performed widefield fluorescence imaging with a system comprising 2 interchangeable LED sources (Thorlabs M470L2, for YFP and M565L3 for dTomato) filtered by 2 interchangeable bandwidth excitation filters (Semrock FF01-452/45 for YFP and F01-545/55-25 for dTomato) and coupled to a diffuser (DG10-1500, Thorlabs) and an achromatic lens (f = 30 mm, #LA1805 Thorlabs). Fluorescence was collected through a tube lens (f = 200 mm), separated from excitation light using a dichroic mirror (Semrock FF510-Di02 for YFP and FF580-FDi01 for dTomato) and detected by a CCD camera (Orca-05G, Hamamatsu) after passing through a visible bandwidth filter (Semrock FF01-609/181 for YFP and FF01-665/150-25 for dTomato).
Chaigneau E., Ronzitti E., Gajowa M.A., Soler-Llavina G.J., Tanese D., Brureau A.Y., Papagiakoumou E., Zeng H, & Emiliani V. (2016). Two-Photon Holographic Stimulation of ReaChR. Frontiers in Cellular Neuroscience, 10, 234.
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4

Intravital Imaging of Somatosensory Cortex

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Live animal imaging was performed as previously described 7 (link), 33 (link), 56 (link). Briefly, a Sutter Movable Objective Microscope (MOM) equipped with a pulsed femtosecond Ti:Sapphire laser (Chameleon Ultra II, Coherent) with two fluorescence detection channels was used for imaging (dichroic beamsplitter: FF520-Di02 (Semrock); blue emission filter: FF01-452/45 (Semrock); green emission filter: ET525/70M (Chroma); photomultiplier tubes: H7422-40 GaAsP (Hamamatsu)). Laser excitation wavelength was set to 830nm. Average laser power was <10-15mW at the tissue surface and adjusted with depth as needed to compensate for signal loss due to scattering and absorption. An Olympus 20× 1.0-NA water immersion objective was used for light delivery and collection. Z-stacks included up to 350 images, acquired at 1μm axial step size, used a 2-frame average, 512 × 512 pixel resolution, and 2.0x-10x zoom (corresponding to 350μm-72μm fields of view). Time-lapse recordings typically included 60–70 images/stack, acquired at 1.0–1.2 μm axial step size, used a 2-frame average, 60 stack repeats (corresponding to approximately 94 min total recording duration), 512 × 512 pixel resolution, and 3.3x-5x zoom (corresponding to 212 μm - 142 μm fields of view). Up to thirteen z-stacks, and four to seven time-lapse recordings were acquired per animal in layers 1 and 2 of the somatosensory cortex.
Huang Y., Happonen K.E., Burrola P.G., O’Connor C., Hah N., Huang L., Nimmerjahn A, & Lemke G. (2021). Microglia use TAM receptors to detect and engulf amyloid beta plaques. Nature immunology, 22(5), 586-594.
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5

High-Resolution Microendoscopic Imaging System

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The HRME system has been previously described in detail [15 (link)]. A bandpass-filtered blue light emitting diode (LED) (FF01-452/45, Semrock, Rochester, NY; M455L2, Thorlabs, Newton, NJ) provides light that passes through a dichroic mirror (485DCLP, Chroma Technology Corp, Bellows Falls, Vt) onto a fiber bundle (FIGH-30-850N, Fujikura, Tokyo, Japan) with a 1-mm outer diameter that is placed in contact with the tissue surface to be imaged. The fiber bundle is composed of 30,000 optical fibers with a 4-μm center-to-center spacing and a 720-μm field of view (FOV). Fluorescence emission returns through the fiber bundle and is imaged through the dichroic mirror and a 550-nm bandpass filter (FF03-550/88, Semrock, Rochester, NY). The emission then passes onto the optical sensor of a charge-coupled device (CCD) camera (GRAS-14S5M, Point Grey, Richmond, Canada). The system has a lateral and axial resolution of 4.4 and 20 μm, respectively. A laptop computer controls the system obtains and displays video at a rate of 15 frames per second.
Shin D., Lee M.H., Polydorides A.D., Pierce M.C., Vila P.M., Parikh N.D., Rosen D.G., Anandasabapathy S, & Richards-Kortum R.R. (2015). Quantitative analysis of high-resolution microendoscopic images for diagnosis of neoplasia in patients with Barrett’s esophagus. Gastrointestinal endoscopy, 83(1), 107-114.
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