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Ab78272

Manufactured by Abcam
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Ab78272 is a mouse monoclonal antibody that recognizes human IKKβ. It can be used for applications such as Western blotting and immunoprecipitation.

Automatically generated - may contain errors

Lab products found in correlation

Ab5095, by Abcam (2 mentions) Ab5131, by Abcam (2 mentions) Actin sc 1615, by Santa Cruz Biotechnology (2 mentions) Sc 9001, by Santa Cruz Biotechnology (2 mentions) Sc 28678, by Santa Cruz Biotechnology (2 mentions) α tubulin 200 301 880, by Rockland Immunochemicals (2 mentions) Sc 484, by Santa Cruz Biotechnology (2 mentions)

2 protocols using ab78272

1

Western Blot Analysis of Transcription

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot analysis was performed as previously described 46 using approximately 30 μg of proteins from cells boiled in Laemmli buffer (50mM Tris pH6.8, 2% sodium dodecyl sulphate, 5% β-mercaptoethanol, 10% glycerol, 0.1% Bromophenol Blue) and antibodies against pol II (sc-9001, Santa Cruz), pSer2 (Ab5095, Abcam), pSer5 (ab5131, Abcam), mitotic phosphoproteins/CC3 (ab78272, Abcam), which recognizes Spt5P 44 (link), Spt5 (sc-28678, Santa Cruz), α-tubulin (200-301-880, Rockland), actin (sc-1615, Santa Cruz), Pcf11 (A303-706A, Bethyl Laboratories), Tyr1P (Active Motif 61384), Thr4P (Active motif 61362), Ser7P (Active motif 61088), Cstf64 (A301-092A, Bethyl Laboratories) and CDK9 (sc-484, Santa Cruz). Validation for all antibodies is provided on the manufacturers’ websites.
Laitem C., Zaborowska J., Isa N.F., Kufs J., Dienstbier M, & Murphy S. (2015). CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes. Nature structural & molecular biology, 22(5), 396-403.
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2

Western Blot Analysis of Transcription

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot analysis was performed as previously described 46 using approximately 30 μg of proteins from cells boiled in Laemmli buffer (50mM Tris pH6.8, 2% sodium dodecyl sulphate, 5% β-mercaptoethanol, 10% glycerol, 0.1% Bromophenol Blue) and antibodies against pol II (sc-9001, Santa Cruz), pSer2 (Ab5095, Abcam), pSer5 (ab5131, Abcam), mitotic phosphoproteins/CC3 (ab78272, Abcam), which recognizes Spt5P 44 (link), Spt5 (sc-28678, Santa Cruz), α-tubulin (200-301-880, Rockland), actin (sc-1615, Santa Cruz), Pcf11 (A303-706A, Bethyl Laboratories), Tyr1P (Active Motif 61384), Thr4P (Active motif 61362), Ser7P (Active motif 61088), Cstf64 (A301-092A, Bethyl Laboratories) and CDK9 (sc-484, Santa Cruz). Validation for all antibodies is provided on the manufacturers’ websites.
Laitem C., Zaborowska J., Isa N.F., Kufs J., Dienstbier M, & Murphy S. (2015). CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes. Nature structural & molecular biology, 22(5), 396-403.
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