Levels of triglyceride, total cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and very low-density lipoprotein (VLDL) were measured using enzymatic assay kits in the UCLA Cardiovascular Core Facility [17 (link)]. The whole mice’s blood was collected with cardiac puncture. The serum was separated from the blood for the detection of pro-inflammatory cytokines using the Quantibody Mouse Cytokine Array kit (RayBiotech, Inc., QAM-CYT-1, Peachtree Corners, GA, USA), which allows the determination of low levels of TNF-α, IL-1β, IL-6, and MCSF from the serum samples.
Quantibody mouse cytokine array kit
Manufactured by RayBiotech
Sourced in United States
The Quantibody Mouse Cytokine Array kit is a multiplex ELISA-based platform designed to quantitatively measure the levels of multiple mouse cytokines and chemokines simultaneously in a single sample. The kit allows for the detection and quantification of various mouse proteins involved in immune response and inflammation.
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Lab products found in correlation
2 protocols using quantibody mouse cytokine array kit
1
Serum Lipid and Cytokine Profile Analysis
2
Quantifying Inflammatory Cytokines in Mice
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The levels of pro-inflammatory cytokines were detected as follows: Briefly, whole mouse blood was collected at 4 or 8 weeks after the final PBS or Pg inoculation with cardiac puncture. The serum was separated from the blood for the detection of pro-inflammatory cytokines using the Quantibody Mouse Cytokine Array kit (RayBiotech, Inc.) which allows for the determination of low levels (<20–30 pg/ml) of cytokines [e.g., granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon (IFN)-γ, IL-1α, IL-1β, IL-6, IL-17, tumor necrosis factor (TNF)-α, vascular endothelial growth factor (VEGF), macrophage colony-stimulating factor (M-CSF), keratinocyte chemoattractant (KC)] from the serum samples.
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