Ubiquitin antibody p4d1

Manufactured by Santa Cruz Biotechnology

The Ubiquitin antibody (P4D1) is a primary antibody that recognizes the ubiquitin protein. Ubiquitin is a small regulatory protein found in all eukaryotic cells and plays a key role in protein degradation and other cellular processes.

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Lab products found in correlation

Ab16667, by Abcam (1 mentions) Alexa fluor 488 donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) P53 do 1, by Santa Cruz Biotechnology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Flag m2 antibody, by Merck Group (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Ubiquitin (134 citations) Ubiquitin antibody (9 citations) Ubiquitin (P4D1, (6 citations) Anti-ubiquitin antibody (P4D1, (6 citations) Mouse monoclonal anti-ubiquitin antibody P4D1 (2 citations) Anti-Ubiquitin antibody P4D1 (sc-8017) (2 citations)

2 protocols using ubiquitin antibody p4d1

Immunofluorescence Antibody Validation Protocol

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All antibodies were purchased from Cell Signaling Technology (Danvers, MA) unless otherwise indicated. These include mouse IgG for α-tubulin (DM1A), V5-Tag (E9H8O), GAPDH (6C5) (Santa Cruz Biotechnology, Santa Cruz, CA), PP2A-C subunit (1D6, EMD Millipore, Burlington, MA), PR55α (2G9, EMD Millipore), p53 (DO1, Santa Cruz Biotechnology), FBXL20 (D-4, Santa Cruz Biotechnology), Ubiquitin antibody (P4D1, Santa Cruz Biotechnology), c-Myc (phospho-S62) (33A12E10, Abcam, Cambridge, UK); rabbit IgG for Ki67 (ab16667, Abcam), p53 (FL-393), PP2A-PR55α Subunit [(100C1) and (#4953)], c-Myc (D3N8F), c-Myc (phospho-T58, ab85380), FBXL20 (SCRAPPER) (ProSci, Poway, CA), FBXL16 Antibody (ProSci). Secondary antibodies for immunofluorescence studies included Alexa Fluor 594 Donkey Anti-rabbit IgG and Alexa Fluor 488 Donkey Anti-mouse IgG from Thermo Fisher Scientific. Nuclei were visualized by DAPI (Sigma-Aldrich, St. Louis, MO).

Madduri L.S., Brandquist N.D., Palanivel C., Talmon G.A., Baine M.J., Zhou S., Enke C.A., Johnson K.R., Ouellette M.M, & Yan Y. (2021). p53/FBXL20 axis negatively regulates the protein stability of PR55α, a regulatory subunit of PP2A Ser/Thr phosphatase. Neoplasia (New York, N.Y.), 23(12), 1192-1203.

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Immunoprecipitation and Ubiquitination Analysis

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293t cells were plated into 10cm dishes. When cells were 50% confluent, cells were transfected with siRNAs listed above (control, Rab40b, Rab40c, or both). After 24 hours, were transfected with FLAG-α-EPLIN using Lipofectamine 2000 (Invitrogen). After 24hrs, cells were treated with 10μM MG132 overnight then lysed in Tris-buffer containing 10mM Iodoacetamide (IAA). Cell lysates were subject to IP with FLAG antibody (M2, Sigma) followed by Western Blot with FLAG or Ubiquitin antibody (P4D1, Santa Cruz).

Linklater E.S., Duncan E.D., Han K., Kaupinis A., Valius M., Lyons T.R., & Prekeris R. (2021). Rab40/Cullin5 complex regulates EPLIN and actin cytoskeleton dynamics during cell migration and invasion.

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