PBMCs were cultured in RPMI-1640 medium (GIBCO, Grand Island, NY, USA) containing 10% FBS, and stimulated with anti-CD3/CD28 (2 μg/mL and 5 μg/mL, Ebioscience) plus Golgiplug (BD Biosciences) for 5 h. The cells were surface-stained with CD3-BV786, CD4-APC-Fire750, CD8-BV421, CD70-PE, and intracellularly stained with TNF-α-BV711 (BD Biosciences), IFN-γ-AF700 (Ebioscience), or IL-2-BV650 (BioLegend) antibodies. For Ki67, perforin or Granzyme B staining, PBMCs were surface-stained with CD3-BV786, CD4-APC-Fire750, CD8-BV421, CD70-PE, and intracellularly stained with Granzyme B-AF700 (BD Biosciences), Ki67-BV711, or perforin-APC (BioLegend) antibodies. A fixable viability dye eFluor® 506 (Ebioscience) was used to assess cell viability.
Cd70 pe
Manufactured by BD
Sourced in United States
CD70-PE is a fluorescently labeled antibody that binds to the CD70 protein. It is designed for use in flow cytometry applications to detect and quantify CD70-expressing cells.
Automatically generated - may contain errors
Lab products found in correlation
Tigit pe cy7, by Thermo Fisher Scientific (2 mentions)
Cd160 af488, by BD (2 mentions)
Cd4 apc fire750, by BD (2 mentions)
2b4 fitc, by BD (1 mentions)
Cd38 bv421, by BioLegend (1 mentions)
Cd45ra af700, by BD (1 mentions)
Cd8 bv510, by BD (1 mentions)
Anti human cd3 bv786, by BD (1 mentions)
Tim 3 bv650, by BD (1 mentions)
Cd28 bv711, by BioLegend (1 mentions)
Cd27 bv650, by BioLegend (1 mentions)
Lag 3 apc, by Thermo Fisher Scientific (1 mentions)
Hla dr af700, by BioLegend (1 mentions)
Cd95 pecy7, by BD (1 mentions)
Ccr7 bv421, by BioLegend (1 mentions)
Pd 1 bv711, by BD (1 mentions)
Lsrfortessa flow cytometer, by BD (1 mentions)
Pd 1 apc, by BD (1 mentions)
Ctla 4 pe, by BD (1 mentions)
Cd56 bv510, by BioLegend (1 mentions)
4 protocols using cd70 pe
1
Phenotypic analysis of activated PBMCs
2
Multiparametric Flow Cytometry of PBMCs
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PBMCs were incubated with directly conjugated antibodies for 30 min at 4°C. The cells were washed before flow cytometry analysis. Antibodies used included anti-human CD3-BV786, CD4-APC-Fire750, CD8-BV510, CD45RA-AF700, CD70-PE, PD-1-BV711, 2B4-FITC, CD160-AF488, TIM-3-BV650, CD95-PE-CY7 (BD Biosciences, San Diego, CA, USA), CCR7-BV421, HLA-DR-AF700, CD38-BV421, CD28-BV711, CD27-BV650 (BioLegend, San Diego, CA, USA), TIGIT-PE-Cy7, LAG-3-APC (Ebioscience, San Diego, CA, USA) and the corresponding isotype controls. Data acquisition was performed on an LSR Fortessa flow cytometer (BD Biosciences), and data was analyzed with FlowJo software (Tree Star, Ashland, OR, USA).
3
Comprehensive NK cell phenotyping
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PBMCs from subjects were resuspended in PBS buffer and were incubated with directly conjugated antibodies for 30 min at 4°C. The cells were washed with 1× PBS before flow cytometry analysis. Antibodies used included anti-human CD3-BV786, CD19-BVAPC-H7, CD16-BV711, CD69 PE-CF594, CD57-BV421, PD-1-APC, CD70-PE, CD160-AF488, 2B4-AF700, CTLA-4-PE, TIM-3-FITC, 7-AAD (BD Biosciences, San Diego, CA, USA), CD56-BV510, BTLA-PE-CY7, CD39-APC (BioLegend, San Diego, CA, USA), TIGIT-PE-Cy7 (Ebioscience, San Diego, CA, USA), along with the corresponding isotype controls. NK cells were gated as CD3−CD14−CD19−CD56+CD16+, CD3−CD14−CD19−CD56+CD16−, or CD3−CD14−CD19−CD56−CD16+. Data were acquired with the LSR Fortessa flow cytometer (BD Biosciences) and analyzed with FlowJo software version 10.5 (Tree Star, Ashland, OR, USA).
4
Comprehensive B-cell Immunophenotyping
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B-cell subsets were analyzed on whole blood by incubating various combinations of monoclonal antibodies, including anti-human CD19 PerCP, anti-IgM APC, CD27 FITC, CD38 FITC, anti-IgD PE, CD21 PE, CD70 PE, CD27 APC, CD24 FITC, CD38 PE, CD183 PE (BD Pharmingen) and CD43 APC (Biolegend, San Diego, CA, USA) monoclonal antibodies, and corresponding isotypes. After staining, blood was lysed by 1 × lysing solution (BD Pharmingen) and washed with phosphate-buffered saline and analyzed. Flow cytometry was performed using FACSCalibur (Becton-Dickenson, San Jose, CA, USA) equipped with argon ion laser emitting at 488 nm (for FITC, PE and APC excitation). Forward and side scatters were used to gate and exclude cellular debris. Ten thousand cells were acquired and analyzed using the Flowjo software (Tree star Inc., Ashland, OR, USA).
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