All histology sectioning and staining was performed at the KI Histology Core. Organs were fixed using formalin immediately after necropsy for 24 hours and stored at 4 °C prior to embedding into paraffin, sectioning, and staining. OVCAR-8 tumour sections were stained with p32 (Genscript custom antibody; 1:100) and NRP-1 (R&D Biosystems AF3870; 1:100). MC-26 tumour sections were stained with αv integrin (Millipore AB1930; 1:100), p32 (AB 2991 Millipore; 1:100), and NRP-1 (AF566 R&D Biosystems; 1:100).
Af3870
Manufactured by R&D Systems
AF3870 is a laboratory equipment product by R&D Systems. It is designed for general laboratory use.
Automatically generated - may contain errors
Lab products found in correlation
Af566, by R&D Systems (2 mentions)
Custom antibody, by R&D Systems (2 mentions)
Ab 2991, by Merck Group (2 mentions)
Ab1930, by Merck Group (2 mentions)
Ab25377, by Abcam (1 mentions)
H 300, by Santa Cruz Biotechnology (1 mentions)
Anti cd66b, by BD (1 mentions)
Sp 8400, by Vector Laboratories (1 mentions)
Nel744b001kt, by PerkinElmer (1 mentions)
3 protocols using af3870
1
Immunohistochemical Analysis of Tumor Samples
2
Immunohistochemical Analysis of Tumor Samples
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All histology sectioning and staining was performed at the KI Histology Core. Organs were fixed using formalin immediately after necropsy for 24 hours and stored at 4 °C prior to embedding into paraffin, sectioning, and staining. OVCAR-8 tumour sections were stained with p32 (Genscript custom antibody; 1:100) and NRP-1 (R&D Biosystems AF3870; 1:100). MC-26 tumour sections were stained with αv integrin (Millipore AB1930; 1:100), p32 (AB 2991 Millipore; 1:100), and NRP-1 (AF566 R&D Biosystems; 1:100).
3
Immunohistochemical Analysis of Lung Tissue
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Murine paraffin-embedded blocks were prepared from lungs fixed via the trachea with 10% buffered formalin at 20 cm H2O. Serial sections were stained with anti-Ly6G clone 1A8 (ab25377, Abcam) and the following polyclonal antibodies: anti-SEMA3F (SAB2700501, Sigma-Aldrich), NRP1 (AF3870, R&D Systems), NRP2 (H300, Santa Cruz Biotechnology), or an isotype control following deparaffinization. Lung sections from patients with COPD undergoing resection for suspected lung tumor were stained with anti-SEMA3F, NRP2, or isotype control, and visualized with DAB or stained with anti-CD66b (555723, BD Biosciences), anti-NRP2 (HPA054974, Atlas Antibodies), and DAPI (422801, Sigma-Aldrich) with TSA plus system amplification (NEL744B001KT, Perkin Elmer) and autofluorescence quenching with TrueView (Vector, SP-8400).
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