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Uv plate

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The UV plate is a laboratory equipment designed to provide controlled ultraviolet (UV) light exposure. It features a UV light source that emits radiation within the UV spectrum, allowing for the uniform illumination of samples or materials placed on the plate.

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Lab products found in correlation

Pampa evolution 96 command software, by Pion Inc (3 mentions) Pampa sandwich, by Pion Inc (2 mentions) Acceptor solution buffer, by Pion Inc (2 mentions) Biomek fx lab, by Beckman Coulter (1 mentions) Biomek fx laboratory automation workstation, by Beckman Coulter (1 mentions)

4 protocols using uv plate

1

Automated PAMPA Permeability Assay

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Parallel Artificial Membrane Permeability Assay (PAMPA) was conducted by Biomek FX lab automation workstation (Beckman Coulter, Inc., Fullerton, CA) and PAMPA evolution 96 command software (pION Inc., Woburn, MA). The detailed method is described as following. 3 mL 10 mM test compound stock in DMSO was mixed with 597 mL of citrate phosphate buffered saline (isotonic) to make diluted test compound. 150 mL of diluted test compound was transferred to a UV plate (pION Inc., Woburn, MA) and the UV spectrum was read as the reference plate. The membrane on pre-loaded PAMPA sandwich (pION Inc., Woburn, MA) was painted with 4 mL GIT lipid (pION Inc., Woburn, MA). The acceptor chamber was then filled with 200 mL ASB (acceptor solution buffer, pION Inc., Woburn, MA), and the donor chamber was filled with 180 mL diluted test compound. The PAMPA sandwich was assembled, placed on the Gut-box and stirred for 30 min. Aqueous Boundary Layer was set to 40 mm for stirring. The UV spectrum (250–500 nm) of the donor and the acceptor were read. The permeability coefficient was calculated using PAMPA evolution 96 command software (pION Inc., Woburn, MA) based on the AUC of the reference plate, the donor plate and the acceptor plate. All compounds were tested in triplicates.
Verlinden B.K., de Beer M., Pachaiyappan B., Besaans E., Andayi W.A., Reader J., Niemand J., van Biljon R., Guy K., Egan T., Woster P.M, & Birkholtz L.M. (2015). Interrogating alkyl and arylalkylpolyamino (bis)urea and (bis)thiourea isosteres as potent antimalarial chemotypes against multiple lifecycle forms of Plasmodium falciparum parasites. Bioorganic & medicinal chemistry, 23(16), 5131-5143.
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2

Automated PAMPA Permeability Assay

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Our parallel artificial membrane permeability assay (PAMPA) was conducted with a Biomek FX Laboratory Automation Workstation (Beckman Coulter, Inc., Fullerton, CA) and PAMPA Evolution 96 Command software (pION Inc., Woburn, MA). The detailed method for this assay was as follows. First, 3 μL of 10 μM test compound stock in DMSO was mixed with 597 μL of citrate PBS (isotonic) to make a diluted test compound. Then, 150 μL of diluted test compound was transferred to a UV plate (pION Inc.), and the UV spectrum of this reference plate was read. The membrane, on a pre-loaded PAMPA Sandwich (pION Inc.), was painted with 4 μL of GIT lipid (pION Inc.). The acceptor chamber was then filled with 200 μL of ASB (acceptor solution buffer; pION Inc.), and the donor chamber was filled with 180 μL of diluted test compound. The PAMPA Sandwich was assembled, placed on the Gut-Box stirring device, and stirred for 30 min. The aqueous boundary layer was set to 40 μm for stirring. The UV spectrum (250–500 nm) of the donor and the acceptor were then read. The permeability coefficient was calculated using PAMPA Evolution 96 Command software, based on the AUCs of the reference plate, the donor plate, and the acceptor plate. All compounds were tested in triplicate.
Hazlitt R.A., Teitz T., Bonga J.D., Fang J., Diao S., Iconaru L., Yang L., Goktug A.N., Currier D.G., Chen T., Rankovic Z., Min J, & Zuo J. (2018). Development of Second-Generation CDK2 Inhibitors for the Prevention of Cisplatin-Induced Hearing Loss. Journal of medicinal chemistry, 61(17), 7700-7709.
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3

Parallel Artificial Membrane Permeability Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The assay was conducted using a Biomek FX lab automation workstation (Beckman Coulter, Inc., Fullerton, CA) with PAMPA evolution 96 command software (pION Inc., Woburn, MA). Test compound stock (10 mM in DMSO, 3 µL) was mixed with citrate phosphate buffered saline (597 µL) to make diluted test compound. Diluted test compound (150 µL) was transferred to a UV plate (pION Inc., Woburn, MA) and the UV spectrum (250–500 nm) was read as the reference plate. Each well of the donor plate in a PAMPA sandwich plate (pION Inc., Woburn, MA) contained a filter that was painted on one side with 4 µL GIT lipid (pION Inc., Woburn, MA) to form a membrane. Each well in the acceptor plate in a PAMPA sandwich, preloaded with magnetic stir bars, was filled with acceptor solution buffer (200 µL, pION Inc., Woburn, MA). The donor plate was filled with diluted test compound (180 µL). The combined PAMPA plate was placed on a pIon Gut-box™ and stirred for 30 min. The UV spectrum (250–500 nm) of the donor and the acceptor were read. The permeability coefficient and recovery were calculated using PAMPA evolution 96 command software (pION Inc., Woburn, MA) based on the whole spectrum measured from the reference plate, the donor plate, and the acceptor plate. All compounds were tested in triplicate.
Chen Y., Zhu F., Hammill J., Holbrook G., Yang L., Freeman B., White K.L., Shackleford D.M., O’Loughlin K.G., Charman S.A., Mirsalis J.C, & Guy R.K. (2021). Selecting an anti-malarial clinical candidate from two potent dihydroisoquinolones. Malaria Journal, 20, 107.
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4

Parallel Artificial Membrane Permeability Assay

Cited 1 time
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A parallel artificial membrane permeability assay (PAMPA) [28 (link),29 (link)] was conducted on a Biomek FX lab automation workstation (Beckman Coulter, Inc.) with PAMPA evolution 96 command software (pION Inc.) as follows: 3 μL of 10 mM test compound stock was mixed with 600 μL of PBS (pH 7.4) to make diluted test compound. Then 150 μL of diluted test compound was transferred to a UV plate (pION Inc.), and the UV spectrum was read as the reference plate. The membrane on a preloaded PAMPA sandwich (pION Inc.) was painted with 4 μL of GIT lipid (pION Inc.). The acceptor chamber was then filled with 200 μL of acceptor solution buffer (pION Inc.), and the donor chamber was filled with 180 μL of diluted test compound. The PAMPA sandwich was assembled, placed on the Gut-Box controlled environment chamber and stirred for 30 min. The aqueous boundary layer was set to 40 μm for stirring. The UV spectrum (250–500 nm) of the donor and the acceptor were read. The permeability coefficient was calculated using PAMPA evolution 96 command software (pION Inc.) based on the AUC of the reference plate, the donor plate, and the acceptor plate. All compounds were tested in triplicate.
Ortiz D., Guiguemde W.A., Johnson A., Elya C., Anderson J., Clark J., Connelly M., Yang L., Min J., Sato Y., Guy R.K, & Landfear S.M. (2015). Identification of Selective Inhibitors of the Plasmodium falciparum Hexose Transporter PfHT by Screening Focused Libraries of Anti-Malarial Compounds. PLoS ONE, 10(4), e0123598.
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