Mouse anti gapdh antibody

Proteins were extracted from liver tissues and HepG2 cells using a cell membrane protein and plasma protein extraction kit (Beyotime, P0033), and the following antibodies were used for Western blot (WB) analyses: BSEP polyclonal antibody (PA5‐78690, Invitrogen, Carlsbad, CA, USA); caveolin‐1 antibody (Cell Signaling Technology, Inc., Danvers, MA, USA); anti‐Hax‐1 antibody (Ab137613, Abcam plc., Cambridge, UK); PKCα antibody (Cell Signaling Technology, Inc.); phospho‐PKCα/β II (Thr638/641) antibody (Cell Signaling Technology, Inc.); mouse anti‐GAPDH antibody (Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China); Na,K‐ATPase antibody (Cell Signaling Technology, Inc.); and mouse anti‐β actin antibody (Zhongshan Golden Bridge Biotechnology Co., Ltd.). Protein expression was normalized to β‐actin, GAPDH and Na⁺, K⁺‐ATPase. Densitometry analyses were performed using the ImageJ software.
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Cell lysates from cultured cells were prepared and analyzed by immunoblotting on nitrocellulose membranes. The following antibodies were used in this study: Mouse total anti-Eg5 T926 (620502) anti-Eg5 total (627802) was purchased from Biolegend. Rabbit anti-cleaved PARP (D64E10) (CS-5625P) and anti phospho histone H3 (ser10) (CST3377) antibody was from Cell Signaling Technologies. Mouse anti-GAPDH antibody was from Zhongshan Golden Bridge Biotechnology (Beijing, China). Anti-rabbit IgG and anti-mouse IgG coupled horseradish peroxidase (HRP) were from Sigma-Aldrich (St. Louis, MO, USA).