Ethanol was removed from samples using a rotary evaporator (Büchi Rotovapor R-205, Büchi Labortechnik AG) equipped with a water bath set at 55 °C (Buchi B-490) and a vacuum pump (Chemglass Scientific Apparatus/10 Torr). Samples were initially concentrated to a thick syrup, then washed by resuspending the preparation in additional water and then re-concentrating. This washing process was done twice in order to achieve the desired ethanol removal (Balto et al., 2016 (link); Pullicin et al., 2018 (link)). The resulting concentrated samples were stored at −23 °C until being lyophilized (Labconco Freezone Freeze Dryer, Hampton, NH).
Rotovapor r 205
Manufactured by Büchi
The Büchi Rotovapor R-205 is a laboratory device designed for the process of rotary evaporation. It is used to efficiently remove solvents from samples through the process of distillation under controlled conditions.
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Lab products found in correlation
2 protocols using rotovapor r 205
1
Ethanol Removal Using Rotary Evaporation
2
Ethanol Removal from MOS Preparations
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Removal of ethanol from the MOS preparations was critical to avoid any interference of residual ethanol with the sensory properties of the final MOS products. Ethanol removal was achieved by solvent removal followed by water washing three times using a rotary evaporator (Büchi Rotovapor R-205, Büchi Labortechnik AG) equipped with a 55° water bath (Buchi B-490) and vacuum pump (Chemglass Scientific Apparatus/10 Torr). Concentrated MOS preparations were stored at -23°C until drying by lyophilization in a VirTis CONSOL 4.5 freeze dryer.
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